125 results on '"Li, Zhou"'
Search Results
2. Single-cell transcriptome analysis profiling lymphatic invasion-related TME in colorectal cancer
- Author
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Liping Wang, Liming Ma, Zhaona Song, Li Zhou, Kexin Chen, Xizi Wang, Zhen Liu, Baozhong Wang, Chen Shen, Xianchao Guo, and Xiaodong Jia
- Subjects
Medicine ,Science - Abstract
Abstract Lymphatic invasion (LI) is extremely aggressive and induces worse prognosis among patients with colorectal cancer (CRC). Thus, it is critical to characterize the cellular and molecular mechanisms underlying LI in order to establish novel and efficacious therapeutic targets that enhance the prognosis of CRC patients. RNA-seq data, clinical and survival information of colon adenocarcinoma (COAD) patients were obtained from the TCGA database. In addition, three scRNA-seq datasets of CRC patients were acquired from the GEO database. Data analyses were conducted with the R packages. We assessed the tumor microenvironment (TME) differences between LI+ and LI− based scRNA-seq data, LI+ cells exhibited augmented abundance of immunosuppression and invasive subset. Marked extracellular matrix network activation was also observed in LI+ cells within SPP1+ macrophages. We revealed that an immunosuppressive and pro-angiogenic TME strongly enhanced LI, as was evidenced by the CD4+ Tregs, CD8+ GZMK+, SPP1+ macrophages, e-myCAFs, and w-myCAFs subcluster infiltrations. Furthermore, we identified potential LI targets that influenced tumor development, metastasis, and immunotherapeutic response. Finally, a novel LIRS model was established based on the expression of 14 LI-related signatures, and in the two testing cohorts, LIRS was also proved to have accurate prognostic predictive ability. In this report, we provided a valuable resource and extensive insights into the LI of CRC. Our conclusions can potentially benefit the establishment of highly efficacious therapeutic targets as well as diagnostic biomarkers that improve patient outcomes.
- Published
- 2024
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- View/download PDF
3. Repurposing non-pharmacological interventions for Alzheimer's disease through link prediction on biomedical literature
- Author
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Yongkang Xiao, Yu Hou, Huixue Zhou, Gayo Diallo, Marcelo Fiszman, Julian Wolfson, Li Zhou, Halil Kilicoglu, You Chen, Chang Su, Hua Xu, William G. Mantyh, and Rui Zhang
- Subjects
Medicine ,Science - Abstract
Abstract Non-pharmaceutical interventions (NPI) have great potential to improve cognitive function but limited investigation to discover NPI repurposing for Alzheimer's Disease (AD). This is the first study to develop an innovative framework to extract and represent NPI information from biomedical literature in a knowledge graph (KG), and train link prediction models to repurpose novel NPIs for AD prevention. We constructed a comprehensive KG, called ADInt, by extracting NPI information from biomedical literature. We used the previously-created SuppKG and NPI lexicon to identify NPI entities. Four KG embedding models (i.e., TransE, RotatE, DistMult and ComplEX) and two novel graph convolutional network models (i.e., R-GCN and CompGCN) were trained and compared to learn the representation of ADInt. Models were evaluated and compared on two test sets (time slice and clinical trial ground truth) and the best performing model was used to predict novel NPIs for AD. Discovery patterns were applied to generate mechanistic pathways for high scoring candidates. The ADInt has 162,212 nodes and 1,017,284 edges. R-GCN performed best in time slice (MR = 5.2054, Hits@10 = 0.8496) and clinical trial ground truth (MR = 3.4996, Hits@10 = 0.9192) test sets. After evaluation by domain experts, 10 novel dietary supplements and 10 complementary and integrative health were proposed from the score table calculated by R-GCN. Among proposed novel NPIs, we found plausible mechanistic pathways for photodynamic therapy and Choerospondias axillaris to prevent AD, and validated psychotherapy and manual therapy techniques using real-world data analysis. The proposed framework shows potential for discovering new NPIs for AD prevention and understanding their mechanistic pathways.
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- 2024
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4. TDF and TAF inhibit liver cancer cell migration, invasion via p7TP3
- Author
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Jing Zhao, Li Zhou, Yang Zhang, Jun Cheng, Yilan Zeng, and Xiuling Li
- Subjects
TDF/TAF ,Liver cancers ,p7TP3 ,Wnt/β-catenin ,Medicine ,Science - Abstract
Abstract Tenofovir disoproxil fumarate (TDF) seems to prevent hepatocellular carcinoma (HCC) in patients with chronic hepatitis B virus (HBV). However, the mechanism is still little known. This study aimed to investigate the the roles and mechanisms of TDF, tenofovir alafenamide fumarate (TAF), and entecavir (ETV) on the malignant characteristics of liver cancer cells. Using the wound-healing assays, transwell assays, matrigel transwell assays, and cell counting kit-8 (CCK-8) assays, it was possible to identify that TDF/TAF, inhibited migration, invasion, and proliferation of HepG2 cells and Huh7 cells. To investigate the mechanisms, we performed TOP/FOP-Flash system, Western blot, and RT-qPCR assays of liver cancer cells cultured with TDF/TAF and found a lower activity of Wnt/β-catenin signaling pathway compared with control cells. Finally, Hepatitis C virus p7 trans-regulated protein 3 (p7TP3), a tumor suppressor in liver cancers, was significantly increased in HepG2 cells and Huh7 cells that treated with TDF/TAF. However, entecavir (ETV)-treated liver cancer cells showed no significant difference in the malignant characteristics of liver cancer cells, activity of Wnt/β-catenin signaling pathway, and expression of p7TP3, compared with the control groups. To conclude, TDF/TAF maybe novel promising therapeutic strategy for liver cancers, including HCC and hepatoblastoma, via Wnt/β-catenin signaling pathway, by up-regulating expression of the tumor suppressor, p7TP3.
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- 2024
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5. Quercetin-crosslinked chitosan nanoparticles: a potential treatment for allergic rhinitis
- Author
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Dehong Mu, Li Zhou, Lingyu Shi, Ting Liu, Ying Guo, Hao Chen, Hongping Luo, Junhao Ma, Hui Zhang, Peizheng Xiong, and Li Tian
- Subjects
Medicine ,Science - Abstract
Abstract Allergic rhinitis (AR) remains a major health problem worldwide. Compared with traditional oral drugs, nasal administration avoids first-pass metabolism and achieve faster and more effective efficacy. In this study, we used the ion crosslinking method to prepare quercetin–chitosan nasal adaptive nanomedicine (QCS) delivery system and evaluated in the treatment of allergic rhinitis mice models. The obtained positively charged nanoparticles with a particle size of 229.2 ± 0.2 nm have excellent characteristics in encapsulation efficiency (79.604%), drug loading rate (14.068%), drug release (673.068 μg) and stability(> 7 days). Excitingly, QCS treatment significantly reduced the number of sneezing and nasal rubbing events in AR mice, while reducing the levels of inflammatory factors such as immunoglobulin E (IgE), interleukin (IL)-17, tumor necrosis factor (TNF)-α, and (IL)-6 to alleviate AR symptoms. Hematoxylin–eosin (HE) staining also showed the damaged nasal mucosa was improved. These experimental results suggest that QCS can effectively suppress allergic inflammation in a mouse model and hold promise as a therapeutic option for allergic rhinitis.
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- 2024
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6. Secondary oxidized di-2-ethylhexyl phthalate metabolites may be associated with progression from isolated premature thelarche to central precocious or early puberty
- Author
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Xiuxin Zheng, Huiping Su, Shurong Huang, Wei Su, Rongfei Zheng, Yue Shang, Qiru Su, Li Zhou, Yao Yao, and Zhe Su
- Subjects
Medicine ,Science - Abstract
Abstract Phthalate esters (PAEs) may act as estrogen receptor agonists, and their relationship with precocious puberty is a global health concern. However, their role in isolated premature thelarche (IPT) progression remains unclear. We conducted a cohort study investigating the relationship between IPT progression and urinary PAE metabolites. Girls with IPT aged 6–8 years were regularly followed up every three months for one year. Clinical data and urine PAE metabolite levels were collected. Participants who progressed to central precocious puberty (CPP) or early puberty (EP) had significantly higher ovarian volume, breast Tanner stage, and levels of the creatinine-adjusted urinary secondary oxidized di-2-ethylhexyl phthalate (DEHP) metabolites (Σ4DEHP). Breast Tanner stage (odds ratio [OR] = 7.041, p = 0.010), ovarian volume (OR = 3.603, p = 0.019), and Σ4DEHP (OR = 1.020, p = 0.005) were independent risk factors for IPT progression. For each 10 µg/g/Cr increase in the urine level of Σ4DEHP, the risk of progression from IPT to CPP/EP within one year increased by 20%. This study demonstrated that the breast Tanner stage, ovarian volume, and Σ4DEHP in urine were independent risk factors for IPT progression, and Σ4DEHP may be associated with the progression of IPT to CPP or EP.
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- 2023
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7. Butein promotes ubiquitination-mediated survivin degradation inhibits tumor growth and overcomes chemoresistance
- Author
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Xin Dong, Wenbin Liu, Xiaoying Li, Yu Gan, Li Zhou, Wei Li, and Li Xie
- Subjects
Medicine ,Science - Abstract
Abstract Overexpression of survivin is frequently observed in human malignancies and is associated with poor prognosis. The present study found that survivin is highly expressed in nasopharyngeal carcinoma (NPC) tumor tissues. Depleting survivin with shRNA inhibited cell viability, colony formation, and in vivo tumorigenesis of NPC cells. With a natural product screening, we identified Butein as a potential anti-tumor compound for NPC by reducing survivin protein level. Butein shortened the half-life of survivin and enhanced ubiquitination-mediated degradation. The mechanism study showed that Butein promoted the interaction between survivin and E3 ligase Fbxl7, and the knockdown of Fbxl7 compromised Butein-induced survivin ubiquitination. Butein suppressed the Akt-Wee1-CDK1 signaling and decreased survivin Thr34 phosphorylation, facilitating E3 ligase Fbxl7-mediated survivin ubiquitination and degradation. Moreover, Butein exhibited a strong in vivo anti-tumor activity, as the tumor volume of Butein-treated xenografts was reduced significantly. Butein alone or combined with cisplatin (CDDP) overcame chemoresistance in NPC xenograft tumors. Overall, our data indicate that Butein is a promising anti-tumor agent for NPC treatment.
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- 2022
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8. IL-6/IL-10 mRNA expression ratio in tumor tissues predicts prognosis in gastric cancer patients without distant metastasis
- Author
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Li Zhou, Chuangang Tang, Xiaoxin Li, and Fang Feng
- Subjects
Medicine ,Science - Abstract
Abstract There was growing evidence that inflammatory responses played significant roles in malignancies. However, the impact of pro-inflammatory-to-anti-inflammatory factor ratio in tumor tissues has not been investigated in gastric cancer (GC) yet. We collected patient data from The Cancer Genome Atlas (TCGA) database. A total of 270 stomach adenocarcinoma (STAD) patients without distant metastasis were included in the study. After screening 12 candidate pro-inflammatory-to-anti-inflammatory pairs, only the IL-6/IL-10 mRNA expression ratio in tumor tissues had a significant effect on overall survival (OS) of STAD patients (P = 0.014). X-tile analysis showed that the greatest survival differences were obtained when the cutoff value of IL-6/IL-10 mRNA expression ratio was set at 1.3 and 5.5. With the low-ratio group (IL-6/IL-10 mRNA expression ratio: 5.5) was significantly shorter (P
- Published
- 2022
- Full Text
- View/download PDF
9. Interpretable predictive model for shield attitude control performance based on XGboost and SHAP
- Author
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Min Hu, Haolan Zhang, Bingjian Wu, Gang Li, and Li Zhou
- Subjects
Medicine ,Science - Abstract
Abstract The sudden decline in the attitude control performance is a common abnormal situation during shield tunneling. When the problem happens, the shield driver will have difficulty controlling the shield's attitude, which will cause the shield to deviate from its design axis and affect the quality of the tunnel. The causes behind poor control performance are usually complicated, so how to choose appropriate countermeasures is a challenging problem. Based on the above issues, this paper proposes the Interpretable Predictive Model for Shield attitude Control Performance (IPM_SCP). The model first predicts the current shield control performance through the extreme gradient boosting (XGBoost) sub-model and then uses the Shapley additive explanation sub-model to interpret the model output. The model was tested on the left-line tunnel of the Hangzhou–Shaoxing railway project in the Ke-Feng section. The results reveal that the model could effectively predict the control performance of the shield and give the most influential parameter and the direction in adjusting the parameter to improve the shield's attitude control performance when the control performance decreases. Therefore, IPM_SCP gives the correct parameter adjustment instructions when the shield’s attitude control performance declines, and eventually improves tunnel construction quality and efficiency.
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- 2022
- Full Text
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10. Association between phthalate exposure and accelerated bone maturation in Chinese girls with early puberty onset: a propensity score-matched case–control analysis
- Author
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Shurong Huang, Zhe Su, Huiping Su, Yanhua Jiao, Qiru Su, Yao Yao, Li Zhou, Xiuxin Zheng, and Xingliang Zhang
- Subjects
Medicine ,Science - Abstract
Abstract Estrogen can promote the acceleration of bone maturation and phthalate esters (PAEs) have estrogen-mimicking effects. We investigated whether PAEs are associated with the acceleration of bone age (BA) in girls with early onset of puberty (EOP). This case–control study enrolled 254 girls with EOP from the Endocrinology Department at Shenzhen Children’s Hospital between December 2018 and August 2019. Ultra-performance liquid chromatography and tandem mass spectrometry were used to analyze the 10 metabolites of PAEs (mPAEs) in urine samples. BA was measured using an artificial intelligence system. BA exceeding the chronological age (CA) by > 2 years (BA-CA ≥ 2 years) was referred to as significant BA advancement. Participants were divided into groups A (BA-CA ≥ 2 years; case group) and B (BA-CA 0.05). Our findings suggested that MEP was positively associated with BA advancement in girls with EOP. Exposure to PAEs may promote accelerated bone maturation.
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- 2022
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11. Retraction Note: Chemical inhibition reveals differential requirements of signaling pathways in krasV12- and Myc-induced liver tumors in transgenic zebrafish
- Author
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Chuan Yan, Qiqi Yang, Xiaojing Huo, Hankun Li, Li Zhou, and Zhiyuan Gong
- Subjects
Medicine ,Science - Published
- 2023
- Full Text
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12. Experimental study and analytical model for the pore structure of epoxy latex-modified mortar
- Author
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Pengfei Li, Wei Lu, Xuehui An, Li Zhou, Xun Han, Sanlin Du, and Chengzhi Wang
- Subjects
Medicine ,Science - Abstract
Abstract Concrete repair and rehabilitation prolong the effective service lives of structures and are important topics in the building field worldwide. Epoxy latex-modified cementitious materials have shown promise for a number of applications in building and construction, but the mix design processes remain arbitrary because their pore structures are not well understood. Porosity and pore size distributions are pore structure parameters that have direct effects on the mechanical properties and durability of concrete. In this paper, mercury intrusion porosimetry (MIP) was used to analyze the porosities and pore size distributions of epoxy latex-modified mortars. The effects of the polymer-to-cement ratio on the pore structures of epoxy latex-modified mortars were investigated. Mortars with polymer-to-cement ratios of 0%, 5%, 10%, 15%, and 20% were cured for 7, 28, 60, and 90 days in this study. Images of specimen microstructures were obtained by scanning electron microscopy (SEM), which showed that increases in the amount of epoxy latex added caused the proportion of micropores in the mortar to decrease, while the proportion of macropores and gel pores increased. The pore size distribution of epoxy latex-modified mortar was described with a composite logarithmic model. Relationships between the pore size distribution and the polymer-to-cement ratio and the curing age were obtained. The method described herein might be sufficiently accurate and convenient to evaluate or predict the pore size distribution of an epoxy latex-modified mortar, i.e., by determining the statistical distribution and analyzing the probability. The process for design of the polymer concrete mix ratio will be facilitated by methods that accurately describe the structure of the epoxy latex-modified mortar.
- Published
- 2022
- Full Text
- View/download PDF
13. Author Correction: Butein promotes ubiquitination-mediated survivin degradation inhibits tumor growth and overcomes chemoresistance
- Author
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Xin Dong, Wenbin Liu, Xiaoying Li, Yu Gan, Li Zhou, Wei Li, and Li Xie
- Subjects
Medicine ,Science - Published
- 2023
- Full Text
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14. Transcriptome analyses revealed molecular responses of Cynanchum auriculatum leaves to saline stress
- Author
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Zhang, Ming, Hong, Li-Zhou, Gu, Min-Feng, Wu, Cheng-Dong, and Zhang, Gen
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- 2020
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15. RETRACTED ARTICLE: Chemical inhibition reveals differential requirements of signaling pathways in krasV12- and Myc-induced liver tumors in transgenic zebrafish
- Author
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Chuan Yan, Qiqi Yang, Xiaojing Huo, Hankun Li, Li Zhou, and Zhiyuan Gong
- Subjects
Medicine ,Science - Abstract
Abstract Previously we have generated inducible liver tumor models by transgenic expression of an oncogene and robust tumorigenesis can be rapidly induced by activation of the oncogene in both juvenile and adult fish. In the present study, we aimed at chemical intervention of tumorigenesis for understanding molecular pathways of tumorigenesis and for potential development of a chemical screening tool for anti-cancer drug discovery. Thus, we evaluated the roles of several major signaling pathways in krasV12- or Myc-induced liver tumors by using several small molecule inhibitors: SU5402 and SU6668 for VEGF/FGF signaling; IWR1 and cardionogen 1 for Wnt signaling; and cyclopamine and Gant61 for Hedgehog signaling. Inhibition of VEGF/FGF signaling was found to deter both Myc- and krasV12-induced liver tumorigenesis while suppression of Wnt signaling relaxed only Myc- but not krasV12-induced liver tumorigenesis. Inhibiting Hedgehog signaling did not suppress either krasV12 or Myc-induced tumors. The suppression of liver tumorigenesis was accompanied with a decrease of cell proliferation, increase of apoptosis, distorted liver histology. Collectively, our observations suggested the requirement of VEGF/FGF signaling but not the hedgehog signaling in liver tumorigenesis in both transgenic fry. However, Wnt signaling appeared to be required for liver tumorigenesis only in Myc but not krasV12 transgenic zebrafish.
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- 2017
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16. Selection of suitable reference genes for qRT-PCR normalisation under different experimental conditions in Eucommia ulmoides Oliv
- Author
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Ye, Jing, Jin, Cang-Fu, Li, Nan, Liu, Min-Hao, Fei, Zhao-Xue, Dong, Li-Zheng, Li, Long, and Li, Zhou-Qi
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- 2018
- Full Text
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17. Stochastic models of routing strategies under the class-based storage policy in fishbone layout warehouses
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Li, Zhou, Junhui, Zhao, Huwei, Liu, Fan, Wang, Jianglong, Yang, and Senhao, Wang
- Subjects
Multidisciplinary - Abstract
In order to improve the picking efficiency of warehouses, shorten the time cost and promote the development of the logistics industry, this study analyzes the routing strategies in fishbone layout warehouses under the class-based storage strategy. The fishbone layout was divided into three storage areas for class A, class B, and class C items according to the proportion using the straight line, to meet the classification requirements of items. Under the class-based storage strategy, to evaluate the performance of the return routing strategy and the S-shape routing strategy, the stochastic models of the expected walking distance of the two routing strategies in the fishbone layout warehouse are established by calculating the sum of the expected walking distances in diagonal cross-aisles and picking aisles. Finally, the stochastic models of the two routing strategies are simulated and verified, and the impacts of the two routing strategies on walking distances are analyzed by comparing the expected distances under different ordering frequencies and space allocation strategies. The numerical results show that the return routing strategy has an advantage over the S-shape routing strategy when determining the relevant parameters of the fishbone layout and picking orders. Meanwhile, it also provides a theoretical basis for research on stochastic models of routing strategies in fishbone layout warehouses under the class-based storage strategy.
- Published
- 2022
18. The beneficial role of Asian-based RecurIndex test in the prognostic prediction in Chinese male breast cancer patients
- Author
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Shuyao Niu, Chao Yang, Xiaohua Du, Shuo Zhang, Li Wang, Chao Song, Furong Du, Beichen Liu, Yueping Liu, Ning Wang, Yunjiang Liu, Jinzhao Liu, Meng-Li Zhou, Jintian Wang, and Jiyu Tang
- Subjects
Male ,Oncology ,medicine.medical_specialty ,Science ,Prognostic prediction ,Risk Assessment ,Article ,Breast Neoplasms, Male ,Tumor grade ,Breast cancer ,Internal medicine ,medicine ,Overall survival ,Humans ,Breast ,Genetic Testing ,Cancer models ,Aged ,Retrospective Studies ,Multidisciplinary ,business.industry ,Distant metastasis ,Middle Aged ,Prognosis ,medicine.disease ,Tissue sections ,Male breast cancer ,T-stage ,Medicine ,Neoplasm Recurrence, Local ,business - Abstract
RecurIndex, a multigene profiling assay, can predict the risk of local recurrence and distant metastasis in female breast cancer (FBC), but its role in male breast cancer (MBC) remains unclear. In this study, the clinicopathological data of 43 consecutive MBC patients undergoing surgeries between 2009 and 2018 were retrospectively analysed. Their paraffin-embedded tissue sections were examined by RecurIndex test which comprised 2 models: recurrence index for local recurrence (RI-LR) and recurrence index for distant recurrence (RI-DR). Of 43 patients, there were 26 low-risk and 17 high-risk patients assessed by RI-LR, while 17 low-risk and 26 high-risk patients by RI-DR. For RI-LR, tumor N stage showed statistically significant (P P = 0.033), T stage (P = 0.043) and N stage (P = 0.003). In terms of clinical outcomes, the overall survival (OS) of low- and high-risk patients stratified by RI-LR showed no statistically significant differences (P = 0.460), while high-risk patients identified by RI-DR had a significantly worse distant recurrence-free survival (DRFS) (P = 0.035), progression-free survival (PFS) (P = 0.019) and OS (P = 0.044) than low-risk patients. Overall, RI-DR can effectively predict the DRFS, PFS and OS of MBC patients and identify those at low risk of recurrence, which may serve as a potential prognostic tool for MBC.
- Published
- 2021
19. Influence of different elicitors on BIA production in Macleaya cordata
- Author
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Wei Liu, Liqiong Xia, Zhixing Qing, Li Zhou, Jianguo Zeng, Peng Huang, and Peng Wang
- Subjects
0106 biological sciences ,0301 basic medicine ,Science ,Cyclopentanes ,Acetates ,01 natural sciences ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,Anti-Infective Agents ,Plant Growth Regulators ,Gene Expression Regulation, Plant ,Papaveraceae ,Sanguinarine ,Oxylipins ,Plant Proteins ,Benzophenanthridines ,Macleaya cordata ,Dihydrobenzophenanthridine oxidase ,Multidisciplinary ,Methyl jasmonate ,Traditional medicine ,biology ,integumentary system ,biology.organism_classification ,Isoquinolines ,Elicitor ,Biosynthetic Pathways ,030104 developmental biology ,chemistry ,Plant stress responses ,Metabolome ,Protopine ,Medicine ,Secondary metabolism ,Salicylic Acid ,Salicylic acid ,010606 plant biology & botany - Abstract
Sanguinarine (SAN) and chelerythrine (CHE) have been widely used as substitutes for antibiotics for decades. For a long time, SAN and CHE have been extracted from mainly Macleaya cordata, a plant species that is a traditional herb in China and belongs to the Papaveraceae family. However, with the sharp increase in demand for SAN and CHE, it is necessary to develop a new method to enhance the supply of raw materials. Here, we used methyl jasmonate (MJ), salicylic acid (SA) and wounding alone and in combination to stimulate aseptic seedlings of M. cordata at 0 h, 24 h, 72 h and 120 h and then compared the differences in metabolic profiles and gene expression. Ultimately, we found that the effect of using MJ alone was the best treatment, with the contents of SAN and CHE increasing by 10- and 14-fold, respectively. However, the increased SAN and CHE contents in response to combined wounding and MJ were less than those for induced by the treatment with MJ alone. Additionally, after MJ treatment, SAN and CHE biosynthetic pathway genes, such as those encoding the protopine 6-hydroxylase and dihydrobenzophenanthridine oxidase enzymes, were highly expressed, which is consistent with the accumulation of SAN and CHE. At the same time, we have also studied the changes in the content of synthetic intermediates of SAN and CHE after elicitor induction. This study is the first systematic research report about using elicitors to increase the SAN and CHE in Macleaya cordata.
- Published
- 2021
20. Whole-transcriptome analysis and construction of an anther development-related ceRNA network in Chinese cabbage (Brassica campestris L. ssp. pekinensis)
- Author
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Fengyan, Shi, Zhijin, Pang, Chuanhong, Liu, Li, Zhou, Chong, Tan, Jie, Ren, Xueling, Ye, Hui, Feng, and Zhiyong, Liu
- Subjects
Multidisciplinary ,Gene Expression Regulation, Plant ,RNA, Plant ,Brassica ,Transcriptome ,Genome-Wide Association Study - Abstract
Anther development is precisely regulated by a complex gene network, which is of great significance to plant breeding. However, the molecular mechanism of anther development in Chinese cabbage is unclear. Here, we identified microRNAs (miRNAs), mRNAs, long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) related to anther development in Chinese cabbage (Brassica campestris L. ssp. pekinensis) to construct competitive endogenous RNA (ceRNA) regulatory networks and provide valuable knowledge on anther development. Using whole-transcriptome sequencing, 9055, 585, 1344, and 165 differentially expressed mRNAs (DEmRNAs), miRNAs (DEmiRNAs), lncRNAs (DElncRNAs), and circRNAs (DEcircRNAs) were identified, respectively, in the anthers of Chinese cabbage compared with those in samples of the vegetative mass of four true leaves. An anther-related ceRNA regulatory network was constructed using miRNA targeting relationships, and 450 pairs of ceRNA relationships, including 97 DEmiRNA–DEmRNA, 281 DEmiRNA–DElncRNA, and 23 DEmiRNA–DEcircRNA interactions, were obtained. We identified important genes and their interactions with lncRNAs, circRNAs, and miRNAs involved in microsporogenesis, tapetum and callose layer development, pollen wall formation, and anther dehiscence. We analyzed the promoter activity of six predominant anther expression genes, which were expressed specifically in the anthers of Arabidopsis thaliana, indicating that they may play an important role in anther development of Chinese cabbage. This study lays the foundation for further research on the molecular mechanisms of anther growth and development in Chinese cabbage.
- Published
- 2022
21. Structural and functional insights into ABHD5, a ligand-regulated lipase co-activator
- Author
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Yan Yuan, Tseng, Matthew A, Sanders, Huamei, Zhang, Li, Zhou, Chia-Yi, Chou, and James G, Granneman
- Subjects
Multidisciplinary ,Protein Conformation ,Mutation ,Computational Biology ,Humans ,Lipase ,Lipid Droplets ,1-Acylglycerol-3-Phosphate O-Acyltransferase ,Ligands - Abstract
Alpha/beta hydrolase domain-containing protein 5 (ABHD5) is a highly conserved protein that regulates various lipid metabolic pathways via interactions with members of the perilipin (PLIN) and Patatin-like phospholipase domain-containing protein (PNPLA) protein families. Loss of function mutations in ABHD5 result in Chanarin–Dorfman Syndrome (CDS), characterized by ectopic lipid accumulation in numerous cell types and severe ichthyosis. Recent data demonstrates that ABHD5 is the target of synthetic and endogenous ligands that might be therapeutic beneficial for treating metabolic diseases and cancers. However, the structural basis of ABHD5 functional activities, such as protein–protein interactions and ligand binding is presently unknown. To address this gap, we constructed theoretical structural models of ABHD5 by comparative modeling and topological shape analysis to assess the spatial patterns of ABHD5 conformations computed in protein dynamics. We identified functionally important residues on ABHD5 surface for lipolysis activation by PNPLA2, lipid droplet targeting and PLIN-binding. We validated the computational model by examining the effects of mutating key residues in ABHD5 on an array of functional assays. Our integrated computational and experimental findings provide new insights into the structural basis of the diverse functions of ABHD5 as well as pathological mutations that result in CDS.
- Published
- 2022
22. Evolutionary patterns of chimeric retrogenes in Oryza species
- Author
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Chengjun Zhang and Yan-Li Zhou
- Subjects
Genetics ,Genome evolution ,Multidisciplinary ,biology ,Retroelements ,Period (gene) ,lcsh:R ,RNA ,lcsh:Medicine ,Oryza ,Chimeric gene ,biology.organism_classification ,Genes, Plant ,Genome ,Article ,Evolution, Molecular ,Plant evolution ,Complementary DNA ,Molecular evolution ,lcsh:Q ,Selection, Genetic ,lcsh:Science ,Gene - Abstract
Chimeric retroposition is a process by which RNA is reverse transcribed and the resulting cDNA is integrated into the genome along with flanking sequences. This process plays essential roles and drives genome evolution. Although the origination rates of chimeric retrogenes are high in plant genomes, the evolutionary patterns of the retrogenes and their parental genes are relatively uncharacterised in the rice genome. In this study, we evaluated the substitution ratio of 24 retrogenes and their parental genes to clarify their evolutionary patterns. The results indicated that seven gene pairs were under positive selection. Additionally, soon after new chimeric retrogenes were formed, they rapidly evolved. However, an unexpected pattern was also revealed. Specifically, after an undefined period following the formation of new chimeric retrogenes, the parental genes, rather than the new chimeric retrogenes, rapidly evolved under positive selection. We also observed that one retro chimeric gene (RCG3) was highly expressed in infected calli, whereas its parental gene was not. Finally, a comparison of our Ka/Ks analysis with that of other species indicated that the proportion of genes under positive selection is greater for chimeric retrogenes than for non-chimeric retrogenes in the rice genome.
- Published
- 2019
23. Experimental study and analytical model for the pore structure of epoxy latex-modified mortar
- Author
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Pengfei Li, Wei Lu, Xuehui An, Li Zhou, Xun Han, Sanlin Du, and Chengzhi Wang
- Subjects
Multidisciplinary - Abstract
Concrete repair and rehabilitation prolong the effective service lives of structures and are important topics in the building field worldwide. Epoxy latex-modified cementitious materials have shown promise for a number of applications in building and construction, but the mix design processes remain arbitrary because their pore structures are not well understood. Porosity and pore size distributions are pore structure parameters that have direct effects on the mechanical properties and durability of concrete. In this paper, mercury intrusion porosimetry (MIP) was used to analyze the porosities and pore size distributions of epoxy latex-modified mortars. The effects of the polymer-to-cement ratio on the pore structures of epoxy latex-modified mortars were investigated. Mortars with polymer-to-cement ratios of 0%, 5%, 10%, 15%, and 20% were cured for 7, 28, 60, and 90 days in this study. Images of specimen microstructures were obtained by scanning electron microscopy (SEM), which showed that increases in the amount of epoxy latex added caused the proportion of micropores in the mortar to decrease, while the proportion of macropores and gel pores increased. The pore size distribution of epoxy latex-modified mortar was described with a composite logarithmic model. Relationships between the pore size distribution and the polymer-to-cement ratio and the curing age were obtained. The method described herein might be sufficiently accurate and convenient to evaluate or predict the pore size distribution of an epoxy latex-modified mortar, i.e., by determining the statistical distribution and analyzing the probability. The process for design of the polymer concrete mix ratio will be facilitated by methods that accurately describe the structure of the epoxy latex-modified mortar.
- Published
- 2021
24. Selective deletion of SHIP-1 in hematopoietic cells in mice leads to severe lung inflammation involving ILC2 cells
- Author
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Fengrui Zhang, Xujun Ye, Zhou Zhu, Lihui Wang, Haiying Tang, Li Zhou, Tao Zheng, William G. Kerr, Zi Chen, Li Zhang, and Yadong Wei
- Subjects
0301 basic medicine ,Cell type ,Science ,Immunology ,Innate lymphoid cells ,Inflammation ,Biology ,Article ,Allergic inflammation ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Papain ,medicine ,Animals ,Lymphocytes ,PI3K/AKT/mTOR pathway ,Mice, Knockout ,Multidisciplinary ,Innate immune system ,Innate lymphoid cell ,Pneumonia ,Hematopoietic Stem Cells ,Fibrosis ,Immunity, Innate ,Asthma ,Mice, Inbred C57BL ,Innate immune cells ,Haematopoiesis ,030104 developmental biology ,Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases ,Cancer research ,Medicine ,medicine.symptom ,030215 immunology - Abstract
Src homology 2 domain–containing inositol 5-phosphatase 1 (SHIP-1) regulates the intracellular levels of phosphotidylinositol-3, 4, 5-trisphosphate, a phosphoinositide 3–kinase (PI3K) product. Emerging evidence suggests that the PI3K pathway is involved in allergic inflammation in the lung. Germline or induced whole-body deletion of SHIP-1 in mice led to spontaneous type 2-dominated pulmonary inflammation, demonstrating that SHIP-1 is essential for lung homeostasis. However, the mechanisms by which SHIP-1 regulates lung inflammation and the responsible cell types are still unclear. Deletion of SHIP-1 selectively in B cells, T cells, dendritic cells (DC) or macrophages did not lead to spontaneous allergic inflammation in mice, suggesting that innate immune cells, particularly group 2 innate lymphoid cells (ILC2 cells) may play an important role in this process. We tested this idea using mice with deletion of SHIP-1 in the hematopoietic cell lineage and examined the changes in ILC2 cells. Conditional deletion of SHIP-1 in hematopoietic cells in Tek-Cre/SHIP-1 mice resulted in spontaneous pulmonary inflammation with features of type 2 immune responses and airway remodeling like those seen in mice with global deletion of SHIP-1. Furthermore, when compared to wild-type control mice, Tek-Cre/SHIP-1 mice displayed a significant increase in the number of IL-5/IL-13 producing ILC2 cells in the lung at baseline and after stimulation by allergen Papain. These findings provide some hints that PI3K signaling may play a role in ILC2 cell development at baseline and in response to allergen stimulation. SHIP-1 is required for maintaining lung homeostasis potentially by restraining ILC2 cells and type 2 inflammation.
- Published
- 2021
25. A full-width half-maximum method to assess retinal vascular structural changes in patients with ischemic heart disease and microvascular angina
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Wen Li Zhou, Li Gang Jiang, Yu Hua Tong, Bo Lun Xu, Ke Yun Cheng, Tie Pei Zhu, Yi Jie Li, and Hai Jing Zhan
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Disease prevention ,lcsh:Medicine ,Disease ,Fundus (eye) ,Chest pain ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Internal medicine ,Arteriovenous ratio ,Medicine ,In patient ,cardiovascular diseases ,Risk factor ,Eye manifestations ,lcsh:Science ,Multidisciplinary ,business.industry ,lcsh:R ,Retinal ,030104 developmental biology ,chemistry ,Cardiology ,lcsh:Q ,medicine.symptom ,business ,Ischemic heart ,030217 neurology & neurosurgery - Abstract
Chest pain patients without obstructive ischemic heart disease (IHD) have increased attention in the clinical practice as carrying higher cardiovascular (CV) risk and impaired life quality. Retinal vasculature is a novel but reliable risk factor of atherosclerosis and systemic vascular diseases. However, the association of retinal blood vessels and unobstructed IHD, as known as microvascular angina (MA) is poorly understood. This study compared retinal vascular structures of obstructive IHD and MA using spectral domain optical coherence tomography (SD-OCT) and full-width half-maximum (FWHM) methods to provide new risk predictive evidence of MA. Fundus vessels of 120 IHD patients, including 91epicardial IHD and 29 MA patients, and 66 control subjects were evaluated. Significant differences in the retinal arterial lumen diameter (RALD), retinal arterial outer diameter (RAOD), and arteriovenous ratio (AVR) have been found (P
- Published
- 2019
26. Metabolic pathways regulated by γ-aminobutyric acid (GABA) contributing to heat tolerance in creeping bentgrass (Agrostis stolonifera)
- Author
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Li, Zhou, Yu, Jingjin, Peng, Yan, and Huang, Bingru
- Published
- 2016
- Full Text
- View/download PDF
27. MicroRNA hsa-mir-3923 serves as a diagnostic and prognostic biomarker for gastric carcinoma
- Author
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Ze Zhang, Li Zhou, Xiaohui Yang, and Lichao Zhang
- Subjects
Male ,0301 basic medicine ,Gene regulatory network ,lcsh:Medicine ,Kaplan-Meier Estimate ,Computational biology ,Biology ,Article ,MiRBase ,Tumour biomarkers ,03 medical and health sciences ,0302 clinical medicine ,Stomach Neoplasms ,microRNA ,Biomarkers, Tumor ,Humans ,Gene Regulatory Networks ,lcsh:Science ,Gene ,Aged ,Neoplasm Staging ,Regulation of gene expression ,Multidisciplinary ,Receiver operating characteristic ,Proportional hazards model ,Gene Expression Profiling ,lcsh:R ,Computational Biology ,Middle Aged ,Prognosis ,Gene Expression Regulation, Neoplastic ,body regions ,Gene expression profiling ,MicroRNAs ,Gene Ontology ,030104 developmental biology ,Risk factors ,ROC Curve ,030220 oncology & carcinogenesis ,Female ,lcsh:Q ,Neoplasm Grading - Abstract
Gastric carcinoma (GC) refers to a common digestive system disease that exhibits a very high incidence. MicroRNA hsa-mir-3923 belongs to a type of miRNA, of which the function has been merely investigated in breast, pancreatic cancers and pre-neoplasic stages of gastric cancer. It has not been studied or reported in gastric carcinoma, so the relationship between gastric hsa-mir-3923 expression and the clinics feature and pathology of GC cases was examined. This study employed data mining for analyzing gastric carcinoma data in The Cancer Genome Atlas database. A Chi squared test was performed for assessing the relations of hsa-mir-3923 expression with clinics-related and pathology-regulated variables. This study conducted the assessment of the role of hsa-mir-3923 in prognostic process using Kaplan–Meier curves, Receiver operating characteristic (ROC) analysis and proportional hazards model (Cox) study. With the use of Gene Expression Omnibus, this study carried out gene set enrichment analysis (GSEA). In the meantime, the common miRNA database was compared to predict potential target genes; as revealed by co-expression analysis, a regulatory network probably existed, containing hsa-mir-3923. For the analysis of the most tightly associated cytological behavior and pathway in GC, this study adopted the databases for Annotation, Visualization and Integrated Discovery (David) and KO-Based Annotation System (KOBAS). Cytoscape, R and STRING were employed for mapping probable regulatory networks displaying relations to hsa-mir-3923. Lastly, we obtained 69 genes most tightly associated with hsa-mir-3923 and described their relationship with Circos plot. As revealed from the results, hsa-mir-3923 displayed up-regulation in gastric carcinoma, and it displayed associations with vital status, N stage and histologic grade when being expressed. The predicted results of miRNA target genes suggested that there may be a close relationship between 66 genes and hsa-mir-3923 in gastric cancer. As indicated from co-expression data, a small regulating network of 4 genes probably existed. Our results elucidated that hsa-mir-3923 high-expression reveals poor prognosis of GC patients.
- Published
- 2020
28. The expression pattern of OsDim1 in rice and its proposed function
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Shu-Xian Gan, Henry Akrofi Doku, Wei Li, Sang Gu Kang, Qian Zhu, Sadia Nadir, Li Zhou, Meng-Ting Li, Eui-Ho Park, Dong-Sun Lee, Lijuan Chen, and Chengyun Li
- Subjects
0106 biological sciences ,0301 basic medicine ,lcsh:Medicine ,Biology ,medicine.disease_cause ,01 natural sciences ,Article ,Plant breeding ,Endosperm ,03 medical and health sciences ,Gene Expression Regulation, Plant ,Pollen ,medicine ,lcsh:Science ,Plant Proteins ,Multidisciplinary ,Hormone activity ,lcsh:R ,fungi ,food and beverages ,Oryza ,Scutellum ,Plants, Genetically Modified ,biology.organism_classification ,Cell biology ,030104 developmental biology ,Seedlings ,Seedling ,Mutation ,Shoot ,lcsh:Q ,RNA Interference ,Pollen tube ,Gene expression ,Elongation ,010606 plant biology & botany - Abstract
Development of plant tissues is dependent on numerous factors, including hormone activity, signaling, cell division, and elongation. In plants, Defective Entry into Mitosis 1 (Dim1) homologs are recognized as pivotal in leaf senescence and progress of normal growth, but their role in rice has not been functionally characterized. The findings presented in this paper suggest that OsDim1 is important in early seedling development, pollen tube elongation, and impacts rice yield components. The gene is expressed in the scutellum, endosperm, embryonic root, shoot, pollen grains and tubes, as well as in several organs of the rice flower. According to the present study findings, RNAi mediated knockdown of OsDim1 resulted in phytohormonal imbalance, reduced amylase activity, affected differentiation of embryonic root elongation zone tissues, suppressed embryonic root and shoot growth, and impaired pollen tube elongation. In contrast, overexpression of OsDim1 showed significant growth in embryonic roots and shoots, while it increased culm length, total number of tillers per plant, seed setting rate, and total number of grains per panicle compared to its wild type line. In summary, we propose OsDim1 plays an important role in seedling growth and pollen tube elongation, and has pleiotropic effects on reproductive tissues.
- Published
- 2019
29. Author Correction: A full-width half-maximum method to assess retinal vascular structural changes in patients with ischemic heart disease and microvascular angina
- Author
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Li Gang Jiang, Tie Pei Zhu, Bo Lun Xu, Yi Jie Li, Yu Hua Tong, Ke Yun Cheng, Wen Li Zhou, and Hai Jing Zhan
- Subjects
Male ,medicine.medical_specialty ,Retinal Artery ,Myocardial Ischemia ,lcsh:Medicine ,Disease ,chemistry.chemical_compound ,Internal medicine ,medicine ,Humans ,In patient ,lcsh:Science ,Author Correction ,Aged ,Multidisciplinary ,business.industry ,lcsh:R ,Microvascular angina ,Retinal Vessels ,Retinal ,Middle Aged ,Full width at half maximum ,chemistry ,Cardiology ,Disease Progression ,lcsh:Q ,Female ,Ischemic heart ,business ,Tomography, Optical Coherence - Abstract
Chest pain patients without obstructive ischemic heart disease (IHD) have increased attention in the clinical practice as carrying higher cardiovascular (CV) risk and impaired life quality. Retinal vasculature is a novel but reliable risk factor of atherosclerosis and systemic vascular diseases. However, the association of retinal blood vessels and unobstructed IHD, as known as microvascular anginga (MA) is poorly understood. This study compared retinal vascular structures of obstructive IHD and MA using spectral domain optical coherence tomography (SD-OCT) and full-width half-maximum (FWHM) methods to provide new risk predictive evidence of MA. Fundus vessels of 120 IHD patients, including 91epicardial IHD and 29 MA patients, and 66 control subjects were evaluated. Significant differences in the retinal arterial lumen diameter (RALD), retinal arterial outer diameter (RAOD), and arteriovenous ratio (AVR) have been found (P 0.05). The severity of IHD was negatively correlated with diameters of RAOD, RALD and AVR (P 0.05). In conclusion, there were significant differences in the retinal vascular structure between IHD patients and patients with MA. Thus, assessment of retinal vascular structure is suggested to evaluate CV risk of IHD patients, despite having no obstructive IHD.
- Published
- 2019
30. Neonatal outcome of children born after ICSI with epididymal or testicular sperm: A 10-year study in China
- Author
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Jin, Lei, primary, Li, Zhou, additional, Gu, Longjie, additional, and Huang, Bo, additional
- Published
- 2020
- Full Text
- View/download PDF
31. Enhanced Second Harmonic Generation by Mode Matching in Gain-assisted Double-plasmonic Resonance Nanostructure
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Gui-Ming Pan, Da-Jie Yang, Zhong-Hua Hao, Qu-Quan Wang, and Li Zhou
- Subjects
Multidisciplinary ,Materials science ,Nanostructure ,business.industry ,Surface plasmon ,lcsh:R ,Resonance ,Second-harmonic generation ,lcsh:Medicine ,02 engineering and technology ,Dielectric ,Fundamental frequency ,021001 nanoscience & nanotechnology ,01 natural sciences ,Article ,010309 optics ,0103 physical sciences ,Harmonic ,Optoelectronics ,lcsh:Q ,0210 nano-technology ,business ,lcsh:Science ,Plasmon - Abstract
We theoretically study the gain-assisted double plasmonic resonances to enhance second harmonic generation (SHG) in a centrosymmetric multilayered silver-dielectric-gold-dielectric (SDGD) nanostructure. Introducing gain media into the dielectric layers can not only compensate the dissipation and lead to giant amplification of surface plasmons (SPs), but also excite local quadrupolar plasmon which can boost SHG by mode matching. Specifically, as the quadrupolar mode dominates SHG in our nanostructure, under the mode matching condition, the intensity of second harmonic near-field can be enhanced by 4.43 × 102 and 1.21 × 105 times when the super-resonance is matched only at the second harmonic (SH) frequency or fundamental frequency, respectively. Moreover, the intensity of SHG near-field is enhanced by as high as 6.55 × 107 times when the nanostructure is tuned to double super-resonances at both fundamental and SH frequencies. The findings in this work have potential applications in the design of nanosensors and nanolasers.
- Published
- 2017
32. The Us2 Gene Product of Herpes Simplex Virus 2 modulates NF-κB activation by targeting TAK1
- Author
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Li Zhou, Changjing Huang, Jingfeng Tang, Qian Li, Xueyu Wang, Yong Lin, Zhang Yi, Shi Liu, and Xuan Lu
- Subjects
0301 basic medicine ,Chemokine ,Immunoprecipitation ,medicine.medical_treatment ,Herpesvirus 2, Human ,Science ,Mutant ,Medizin ,Article ,Cell Line ,Serine ,Gene product ,03 medical and health sciences ,Viral Envelope Proteins ,Protein Interaction Mapping ,medicine ,Animals ,Humans ,Mice, Inbred BALB C ,Multidisciplinary ,Herpes Genitalis ,biology ,Chemistry ,Kinase ,NF-kappa B p50 Subunit ,MAP Kinase Kinase Kinases ,Virology ,Cell biology ,Disease Models, Animal ,030104 developmental biology ,Cytokine ,Host-Pathogen Interactions ,biology.protein ,Phosphorylation ,Medicine ,Female ,Protein Binding - Abstract
HSV-2 is one of the most common sexually transmitted pathogens worldwide and HSV-2 infection triggers cytokine and chemokine production. However, little is known about which HSV-2 genes engage in the regulation of NF-κB signaling and what mechanisms are involved. In a screen of the unique short (Us) regions of HSV-2, we observed that HSV-2 Us2 activates NF-κB signaling. We additionally indicated that deficiencies of Us2 decrease HSV-2 WT mediated NF-κB activation and cytokine and chemokine production, and overexpression of Us2 showed opposite effects. Co-immunoprecipitations indicated that Us2 interacted with TGF-β activated kinase 1 (TAK1), a serine/threonine kinase essential for NF-κB activation, and Us2 has the ability to regulate the TAK1-mediated pathway and induces TAK1 downstream signaling. Further studies verified that Us2 induced the phosphorylation of TAK1, resulting in the activation of TAK1 mediated downstream signaling. The role of Us2 in HSV-2 induced NF-κB pathways was also confirmed in the Us2-deficient mutant and HSV-2 WT infected mice. Our results indicate that HSV-2 Us2 gene product binds to TAK1 to positively regulate NF-κB signaling and, for the first time, provide insights into the molecular mechanism.
- Published
- 2017
33. Plasmon-Enhanced Photoelectrochemical Current and Hydrogen Production of (MoS2-TiO2)/Au Hybrids
- Author
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Liang Ma, Qu-Quan Wang, Jiahong Wang, Si-Jing Ding, Yingying Li, Zhi-Jun Luo, Kai Chen, Zi-Qiang Cheng, and Li Zhou
- Subjects
Multidisciplinary ,Nanocomposite ,Materials science ,business.industry ,Science ,Nanotechnology ,02 engineering and technology ,Substrate (electronics) ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Semiconductor ,Photocatalysis ,Medicine ,Nanorod ,Surface plasmon resonance ,0210 nano-technology ,business ,Plasmon ,Hydrogen production - Abstract
Three component hybrid (MoS2-TiO2)/Au substrate is fabricated by loading plasmonic Au nanorods on the MoS2 nanosheets coated TiO2 nanorod arrays. It is used for photoelectrochemical (PEC) cell and photocatalyst for hydrogen generation. Owing to the charge transfer between the MoS2-TiO2 hetero-structure, the PEC current density and hydrogen generation of TiO2 nanoarrays are enhanced 2.8 and 2.6 times. The broadband photochemical properties are further enhanced after Au nanorods loading. The plasmon resonance of Au nanorods provides more effective light-harvesting, induces hot-electron injection, and accelerates photo-excited charges separation. The results have suggested a route to construct nanohybrid by combining one-dimensional arrays and two-dimensional nanosheets, meanwhile have successfully utilized plasmonic nanorods as a sensitizer to improve the photochemical properties of the semiconductor nanocomposite.
- Published
- 2017
34. Clinical and Microbiological Characteristics of Group B Streptococcus from Pregnant Women and Diseased Infants in Intrapartum Antibiotic Prophylaxis Era in Taiwan
- Author
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Reyin Lien, Chien-Chung Lee, Jen-Fu Hsu, Chyi-Liang Chen, Ying-Li Zhou, Rajendra Prasad Janapatla, and Cheng-Hsun Chiu
- Subjects
Adult ,Male ,0301 basic medicine ,Serotype ,medicine.medical_specialty ,Taiwan ,lcsh:Medicine ,Microbial Sensitivity Tests ,Disease ,Serogroup ,medicine.disease_cause ,Infant, Newborn, Diseases ,Article ,Group B ,Streptococcus agalactiae ,03 medical and health sciences ,0302 clinical medicine ,Pregnancy ,Streptococcal Infections ,Internal medicine ,medicine ,Humans ,Colonization ,Antibiotic prophylaxis ,Clinical microbiology ,lcsh:Science ,Multidisciplinary ,Streptococcus ,business.industry ,lcsh:R ,Infant, Newborn ,Infant ,Antibiotic Prophylaxis ,medicine.disease ,Anti-Bacterial Agents ,030104 developmental biology ,medicine.anatomical_structure ,Vagina ,Female ,lcsh:Q ,business ,Microbial genetics ,Meningitis ,030217 neurology & neurosurgery ,Multilocus Sequence Typing - Abstract
Group B Streptococcus (GBS) is one of the most important pathogens for neonates. This study included 69 invasive GBS diseases in neonates, including 7 early-onset disease (EOD), 55 late-onset disease, and 7 very-late-onset disease from 2013 to 2017. A significant reduction of EOD after the deployment of intrapartum antibiotic prophylaxis (IAP) in 2012 was observed. A previously-recognized hypervirulent clone GBS III ST17, accounting for 68% of the overall infections and 71% of the meningitis, was identified among the 69 cases. A novel GBS Ia ST890 emerged, becoming the fourth most common clone. Overall 96% of the invasive GBS infections were caused by serotypes Ia, Ib, and III. We collected 300 GBS isolates from vagina of the healthy pregnant women in 2014 and 2017. The serotype distribution of the maternal colonization isolates was VI (35%), III (21%), V (15%), Ib (13%) and Ia (11%) in 2014, and VI (32%), III (22%), V (16%), Ia (16%), and Ib (8%) in 2017. The most common sequence types were ST1 (32%), ST12 (22%), and ST23 (15%). Serotype diversity of maternal colonization strains did not change between 2014 and 2017. The study provides useful information in surveillance of GBS disease in the era of IAP.
- Published
- 2019
35. Genome-wide Identification, Classification, Expression and Duplication Analysis of GRAS Family Genes in Juglans regia L
- Author
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Yang Qin, Li Ma, Hang Xu, Li Zhou, Shaowen Quan, and Jianxin Niu
- Subjects
0301 basic medicine ,Plant molecular biology ,Science ,Meristem ,Juglans ,Genome ,Article ,03 medical and health sciences ,0302 clinical medicine ,Gene Expression Regulation, Plant ,Phylogenetics ,Gene Duplication ,Gene duplication ,Gene family ,Gene ,Phylogeny ,Plant Proteins ,Regulation of gene expression ,Genetics ,Shoot apical meristem ,Multidisciplinary ,biology ,Gene Expression Profiling ,Gene Expression Regulation, Developmental ,biology.organism_classification ,Gene expression profiling ,030104 developmental biology ,Multigene Family ,Medicine ,Genome, Plant ,030217 neurology & neurosurgery ,Transcription Factors - Abstract
Fifty-two GRAS genes are identified in walnut genome. Based on the evolutionary relationship and motif analysis, the walnut GRAS gene family was divided into eight subfamilies, and the sequence features analysis of JrGRAS proteins showed that the JrGRAS protein sequences were both conserved and altered during the evolutionary process. Gene duplication analysis indicated that seven GRAS genes in walnut have orthologous genes in other species, and five of them occurred duplicated events in walnut genome. Expression pattern analysis of the GRAS family genes in walnut showed that two JrGRAS genes (JrCIGRa-b and JrSCL28a) were differentially expressed between flower bud and leaf bud (p JrGRAS genes (JrCIGRa-b and JrSCL13b-d) were differentially expressed between the different development stages of flower buds transition (p JrGAIa, JrSCL3f and JrSHRc) were identified by co-expression analysis, which suggested these GRAS genes may play an important role in regulating the development of apical meristem in walnut. This study laid a foundation for further understanding of the function of GRAS family genes in walnut.
- Published
- 2019
36. Stages identifying and transcriptome profiling of the floral transition in Juglans regia
- Author
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Yang Qin, Li Ma, Shaowen Quan, Hang Xu, Jianxin Niu, and Li Zhou
- Subjects
0301 basic medicine ,lcsh:Medicine ,Juglans ,Flowers ,Genes, Plant ,Real-Time Polymerase Chain Reaction ,Flowering time ,Article ,Transcriptome ,03 medical and health sciences ,0302 clinical medicine ,Gene Expression Regulation, Plant ,Paraffin section ,Transcriptome profiling ,Gene Regulatory Networks ,Primordium ,RNA-Seq ,lcsh:Science ,Plant Proteins ,Shoot apical meristem ,Multidisciplinary ,biology ,Bud ,lcsh:R ,Gene Expression Regulation, Developmental ,RNA sequencing ,biology.organism_classification ,Plant Leaves ,Horticulture ,030104 developmental biology ,Differentially expressed genes ,lcsh:Q ,030217 neurology & neurosurgery ,Transcription Factors - Abstract
Using paraffin sections, the stages of walnut female flower bud differentiation were divided into the predifferentiation period (F_1), initial differentiation period (F_2) and flower primordium differentiation period (F_3). Leaf buds collected at the same stage as F_2 were designated JRL. Transcriptomic profiling was performed, and a total of 132,154 unigenes were obtained with lengths ranging from 201 bp to 16,831 bp. The analysis of differentially expressed genes (DEGs) showed that there were 597, 784 and 532 DEGs in the three combinations F_1vsF_2, F_1vsF_3, and F_2vsF_3, respectively. The comparison F_2vsJRL showed that 374 DEGs were differentially expressed between female buds and leaf buds. Thirty-one DEGs related to flowering time were further used to construct coexpression networks, and CRY2 and NF-YA were identified as core DEGs in flowering time regulation. Eighteen DEGs related to flowering time were subjected to real-time quantitative analysis. Our work provides a foundation for further research on the walnut floral transition and provides new resources for future research on walnut biology and biotechnology.
- Published
- 2019
37. The cotton WRKY transcription factor (GhWRKY33) reduces transgenic Arabidopsis resistance to drought stress
- Author
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Xue-Bao Li, Yun-Lue Sun, Na-Na Wang, Dong Liu, Shang-Wei Xu, Li Zhou, and Yang Li
- Subjects
0301 basic medicine ,Science ,Transgene ,Arabidopsis ,Genetically modified crops ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Gene Expression Regulation, Plant ,Stress, Physiological ,Promoter Regions, Genetic ,Abscisic acid ,Transcription factor ,Gossypium ,Multidisciplinary ,biology ,Arabidopsis Proteins ,fungi ,Wild type ,food and beverages ,Promoter ,Salt Tolerance ,Plants, Genetically Modified ,biology.organism_classification ,Adaptation, Physiological ,WRKY protein domain ,Droughts ,Cell biology ,030104 developmental biology ,chemistry ,Medicine ,030217 neurology & neurosurgery ,Abscisic Acid ,Signal Transduction ,Transcription Factors - Abstract
As the important source of natural fibers in the textile industry, cotton fiber quality and yield are often restricted to drought conditions because most of cotton plants in the world grow in the regions with water shortage. WRKY transcription factors regulate multiple plant physiological processes, including drought stress response. However, little is known of how the WRKY genes respond to drought stress in cotton. Our previous study revealed GhWRKY33 is leaf-specific and induced by drought stress. In this study, our data showed GhWRKY33 protein localizes to the cell nucleus and is able to bind to “W-box” cis-acting elements of the target promoters. Under drought stress, GhWRKY33 overexpressing transgenic Arabidopsis was withered much more quickly than wild type due to faster water loss. Moreover, GhWRKY33 transgenic plants displayed more tolerance to abscisic acid (ABA), relative to wild type. Expression of some drought stress-related genes and ABA-responsive genes were changed in the GhWRKY33 transgenic Arabidopsis with drought or ABA treatment. Collectively, our findings indicate that GhWRKY33 may act as a negative regulator to mediate plant response to drought stress and to participate in the ABA signaling pathway.
- Published
- 2019
38. Imaging Dose, Cancer Risk and Cost Analysis in Image-guided Radiotherapy of Cancers
- Author
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Li Zhou, Yibao Zhang, Jun Deng, Xin Ming, Ying Zhang, and S. Bai
- Subjects
Thorax ,Male ,Cone beam computed tomography ,medicine.medical_treatment ,Cost-Benefit Analysis ,lcsh:Medicine ,030218 nuclear medicine & medical imaging ,0302 clinical medicine ,Risk Factors ,Neoplasms ,lcsh:Science ,Child ,Lung ,Aged, 80 and over ,Multidisciplinary ,Phantoms, Imaging ,Brain ,Radiotherapy Dosage ,Cone-Beam Computed Tomography ,Middle Aged ,Leukemia ,030220 oncology & carcinogenesis ,Child, Preschool ,Female ,Radiology ,Monte Carlo Method ,Adult ,medicine.medical_specialty ,Adolescent ,Bone Marrow Cells ,Radiation Dosage ,Article ,03 medical and health sciences ,Young Adult ,medicine ,Humans ,Lung cancer ,Aged ,business.industry ,lcsh:R ,Cancer ,Infant ,Retrospective cohort study ,medicine.disease ,Radiation therapy ,Attributable risk ,lcsh:Q ,business ,Radiotherapy, Image-Guided - Abstract
The purpose of this retrospective study is to evaluate the cumulative imaging doses, the associated cancer risk and the cost related to the various radiological imaging procedures in image-guided radiotherapy of cancers. Correlations between patients’ size and Monte Carlo simulated organ doses were established and validated for various imaging procedures, and then used for patient-specific organ dose estimation of 4,832 cancer patients. The associated cancer risk was estimated with published models and the cost was calculated based on the standard billing codes. The average (range) cumulative imaging doses to the brain, lungs and red bone marrow were 38.0 (0.5–177.3), 18.8 (0.4–246.5), and 49.1 (0.4–274.4) cGy, respectively. The associated average (range) lifetime attributable risk of cancer incidence per 100,000 persons was 78 (0–2798), 271 (1–8948), and 510 (0–4487) for brain cancer, lung cancer and leukemia, respectively. The median (range) imaging cost was $5256 (4268–15896) for the head scans, $5180 (4268–16274) for the thorax scans, and $7080 (4268–15288) for the pelvic scans, respectively. The image-guidance procedures and the accumulated imaging doses should be incorporated into clinical decision-making to personalize radiotherapy for individual patients.
- Published
- 2018
39. Comparison of two different ecological floating bio-reactors for pollution control in hyper-eutrophic freshwater
- Author
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Cai Min, Guoyan Zou, Cui Naxin, Xiangfu Song, Guifa Chen, Li Zhou, and Liu Yaqin
- Subjects
Pollution ,Time Factors ,Nitrogen ,Microorganism ,media_common.quotation_subject ,0208 environmental biotechnology ,lcsh:Medicine ,chemistry.chemical_element ,Fresh Water ,02 engineering and technology ,010501 environmental sciences ,01 natural sciences ,Ammonia ,chemistry.chemical_compound ,Bioreactors ,Microbial biodegradation ,Cyperus ,lcsh:Science ,Water pollution ,Nitrogen cycle ,0105 earth and related environmental sciences ,media_common ,Multidisciplinary ,Chemistry ,Ecology ,lcsh:R ,Water Pollution ,Phosphorus ,Eutrophication ,020801 environmental engineering ,lcsh:Q ,Water Pollutants, Chemical - Abstract
The use of ecological floating beds (EFBs) to control water pollution has been increasingly reported worldwide due to the severe situation of eutrophication in water bodies. In this study, two kinds of EFBs were set up under similar condition to compare their purification efficiency in hyper-eutrophic water. The conventional ecological floating bed (CEFB) was made of polystyrene foam board, and the enhanced ecological floating bio-reactor (EEFB) was designed as an innovative hollow, thin floating bed integrated with substrates of zeolite and limestone. The results showed that the EEFB increased treatment efficiency of total nitrogen (TN), total phosphate (TP), and ammonia nitrogen (NH4+-N) to 63.5%, 59.3%, and 68.0%, respectively. Plant accumulation was the main pathway for TN and TP removal in the CEFB. Microbial degradation played an increasingly important role in TN and TP removal in the EEFB. A higher concentration of nitrogen cycling bacteria was recorded in the EEFB than the CEFB (P
- Published
- 2018
40. Diterpenoid Alkaloids from Delphinium anthriscifolium var. majus
- Author
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Ji-Fa Zhang, Lianhai Shan, Xian-Li Zhou, Shuai Huang, and Feng Gao
- Subjects
Spectrometry, Mass, Electrospray Ionization ,Magnetic Resonance Spectroscopy ,Science ,01 natural sciences ,Article ,Cell Line ,Evolution, Molecular ,Alkaloids ,Humans ,Multidisciplinary ,Traditional medicine ,biology ,010405 organic chemistry ,Chemistry ,Alkaloid ,Delphinium anthriscifolium ,Delphinium ,biology.organism_classification ,Terpenoid ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,Phytochemical ,Medicine ,Diterpenes ,Cancer cell lines - Abstract
Extensive phytochemical investigation on the whole herbs of Delphinium anthriscifolium var. majus led to the identification of fourteen diterpenoid alkaloids, including three new C20–diterpenoid alkaloids (anthriscifolsines A–C, 1–3), six new C19–diterpenoid alkaloids (anthriscifolrines A–F, 4–9), and five know compounds (10–14). Among them, anthriscifolsine A represents a novel C20–diterpenoid alkaloid characterized by a seco C–ring. The structures of the isolated compounds were elucidated by extensive spectroscopic methods, including HR-ESI–MS, X–ray, and 1D and 2D NMR experiments. Bioactivity of compounds 3–6 was evaluated for their cytotoxicity against the MCF–7, HepG2 and H460 cancer cell lines.
- Published
- 2017
41. Wider geographic distribution and higher diversity of hexaploids than tetraploids in Carassius species complex reveal recurrent polyploidy effects on adaptive evolution
- Author
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Xiao-Juan Zhang, Xiao-Li Liu, Zhong-Wei Wang, Xi-Yin Li, Jian-Feng Mao, Li Zhou, Jian-Fang Gui, Fan Chen, Fang-Fang Jiang, and Zhi Li
- Subjects
0301 basic medicine ,Fish Proteins ,Species complex ,China ,Science ,Population ,Cyprinidae ,Gene Expression ,Context (language use) ,Biology ,Article ,Nucleotide diversity ,Polyploidy ,03 medical and health sciences ,Polyploid ,Rivers ,Genetic variation ,Animals ,education ,Ecosystem ,Phylogeny ,Genetics ,education.field_of_study ,Multidisciplinary ,Altitude ,Transferrin ,food and beverages ,Genetic Variation ,Humidity ,Adaptation, Physiological ,Phylogeography ,030104 developmental biology ,Genetics, Population ,Sympatric speciation ,Evolutionary biology ,Medicine ,Adaptation - Abstract
Polyploidy roles on adaptive evolution and ecological novelty have been extensively studied in plants but remained unclear in vertebrates owing to the rare polyploidy incidences. Here, a huge number of 3105 specimens in Carassius species complex including 2211 hexaploids and 894 tetraploids were sampled from 34 locations through mainland China. And hexaploids had wider geographic distribution than tetraploids especially in the areas with high altitude, high latitude and low annual precipitation. Then, an approximate 1050 bp transferrin (tf) fragments were amplified from all the samples, and 526 tf alleles were identified from a total of 37260 sequences at last. Intriguingly, higher nucleotide diversity of tf alleles in hexaploids than in tetraploids was revealed. Moreover, via phylogenetic analysis of tf alleles, potential origin center of Carassius species complex was deduced to be Yangtze River basin and hexaploids should undergo multiple independent polyploidy origins from sympatric tetraploids. These findings indicate that the hexaploids might possess stronger environmental adaptation and ecological novelty than the tetraploids, which provide an association paradigm of recurrent polyploidy and ecological context in polyploid vertebrates.
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- 2017
42. Human Gingiva-Derived Mesenchymal Stem Cells Ameliorate Streptozoticin-induced T1DM in mice via Suppression of T effector cells and Up-regulating Treg Subsets
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Nancy J. Olsen, Dongqing Li, Junlong Dang, Julie Wang, Jilin Ma, Jia Yuan, Wei Zhang, Yanming Chen, Ximei Zhang, Weiwen Chen, Jichao Liang, Homayoun H. Zadeh, Li Zhou, and Song Guo Zheng
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0301 basic medicine ,Male ,Gingiva ,lcsh:Medicine ,Spleen ,CD8-Positive T-Lymphocytes ,Mesenchymal Stem Cell Transplantation ,T-Lymphocytes, Regulatory ,Article ,Diabetes Mellitus, Experimental ,Pancreatic Lymph Node ,03 medical and health sciences ,Mice ,0302 clinical medicine ,medicine ,Animals ,Humans ,lcsh:Science ,Lymph node ,Multidisciplinary ,business.industry ,Mesenchymal stem cell ,lcsh:R ,Mesenchymal Stem Cells ,Streptozotocin ,030104 developmental biology ,medicine.anatomical_structure ,Diabetes Mellitus, Type 1 ,Cancer research ,Heterografts ,lcsh:Q ,Lymph ,Pancreas ,business ,CD8 ,030215 immunology ,medicine.drug - Abstract
There is yet no cure for type 1 diabetes (T1DM) so far. A significant body of evidence has demonstrated that bone marrow-derived mesenchymal stem cells (BMSCs) showed great potential in controlling T1DM. But there exists much difficulty in using BMSCs as a clinical therapy. We here test whether a new population of mesenchymal stem cells from human gingiva (GMSCs), which has many advantages over BMSCs, can delay or prevent progress of T1DM. GMSCs were adoptively transferred to multiple low-dose streptozotocin (STZ)-induced T1DM. Blood glucose levels and disease severities were analyzed. T cells subsets in blood, spleen and lymph nodes were detected dynamically by flow cytometry. GMSC distribution was dynamically analyzed. We found that infusion of GMSCs but not fibroblast cells significantly controlled blood glucose levels, delayed diabetes onset, ameliorated pathology scores in pancreas, and down-regulated production of IL-17 and IFN-γ in CD4+ and CD8+ T cells in spleens, pancreatic lymph nodes (pLN) and other lymph nodes. GMSCs also up-regulated the levels of CD4+ Treg induced in the periphery. Mechanismly, GMSCs could migrate to pancreas and local lymph node and function through CD39/CD73 pathway to regulate effector T cells. Thus, GMSCs show a potential promise in treating T1DM in the clinic.
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- 2017
43. Plasmon-Enhanced Photoelectrochemical Current and Hydrogen Production of (MoS
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Ying-Ying, Li, Jia-Hong, Wang, Zhi-Jun, Luo, Kai, Chen, Zi-Qiang, Cheng, Liang, Ma, Si-Jing, Ding, Li, Zhou, and Qu-Quan, Wang
- Subjects
Article - Abstract
Three component hybrid (MoS2-TiO2)/Au substrate is fabricated by loading plasmonic Au nanorods on the MoS2 nanosheets coated TiO2 nanorod arrays. It is used for photoelectrochemical (PEC) cell and photocatalyst for hydrogen generation. Owing to the charge transfer between the MoS2-TiO2 hetero-structure, the PEC current density and hydrogen generation of TiO2 nanoarrays are enhanced 2.8 and 2.6 times. The broadband photochemical properties are further enhanced after Au nanorods loading. The plasmon resonance of Au nanorods provides more effective light-harvesting, induces hot-electron injection, and accelerates photo-excited charges separation. The results have suggested a route to construct nanohybrid by combining one-dimensional arrays and two-dimensional nanosheets, meanwhile have successfully utilized plasmonic nanorods as a sensitizer to improve the photochemical properties of the semiconductor nanocomposite.
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- 2017
44. Tuning of Exchange Coupling and Switchable Magnetization Dynamics by Displacing the Bridging Ligands Observed in Two Dimeric Manganese(III) Compounds
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Xiangyu Liu, Gang Xie, Li-Zhou Wu, Feifei Li, Weiming Song, Peipei Cen, and Sanping Chen
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Magnetization dynamics ,Multidisciplinary ,Materials science ,010405 organic chemistry ,chemistry.chemical_element ,Manganese ,010402 general chemistry ,01 natural sciences ,Article ,0104 chemical sciences ,Ion ,Metal ,chemistry.chemical_compound ,Magnetization ,Crystallography ,chemistry ,Ferromagnetism ,visual_art ,visual_art.visual_art_medium ,Antiferromagnetism ,Carboxylate - Abstract
Two Mn(III)-based dimers, [Mn2(bpad)2(CH3O)4]n (1) and [Mn2(bpad)2(pa)2]n·2H2O (2) (Hbpad = N3-benzoylpyridine-2-carboxamidrazone, H2pa = phthalic acid), have been assembled from a tridentate Schiff-base chelator and various anionic coligands. Noteworthily, compound 1 could be identified as a reaction precursor to transform to 2 in the presence of phthalic acid, resulting in a rarely structural conversion process in which the bridges between intradimer Mn(III) ions alter from methanol oxygen atom with μ2-O mode in 1 (Mn "Equation missing" Mn distance of 3.046 Å) to syn-anti carboxylate in 2 (Mn "Equation missing" Mn distance of 4.043 Å), while the Mn(III) centers retain hexa-coordinated geometries with independently distorted octahedrons in two compounds. The dc magnetic determinations reveal that ferromagnetic coupling between two metal centers with J = 1.31 cm−1 exists in 1, whereas 2 displays weak antiferromagnetic interactions with the coupling constant J of −0.56 cm−1. Frequency-dependent ac susceptibilities in the absence of dc field for 1 suggest slow relaxation of the magnetization with an energy barrier of 13.9 K, signifying that 1 features single-molecule magnet (SMM) behavior. This work presents a rational strategy to fine-tune the magnetic interactions and further magnetization dynamics of the Mn(III)-containing dinuclear units through small structural variations driven by the ingenious chemistry.
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- 2017
45. Plasmon-assisted site-selective growth of Ag nanotriangles and Ag-Cu2O hybrids
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Qu-Quan Wang, Liang Ma, Zhong-Hua Hao, Da-Jie Yang, Ying Xie, Zi-Qiang Cheng, and Li Zhou
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Multidisciplinary ,Materials science ,Nanocomposite ,business.industry ,chemistry.chemical_element ,Nanoparticle ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Photochemistry ,01 natural sciences ,Copper ,0104 chemical sciences ,Metal ,Semiconductor ,chemistry ,visual_art ,visual_art.visual_art_medium ,Surface plasmon resonance ,0210 nano-technology ,business ,Local field ,Plasmon - Abstract
We report a plasmon-assisted growth of metal and semiconductor onto the tips of Ag nanotriangles (AgNTs) under light irradiation. The site-selective growth of Ag onto AgNTs are firstly demonstrated on the copper grids and amine-coated glass slides. As the irradiation time increases, microscopic images indicate that AgNTs gradually touch with each other and finally “weld” tip-to-tip together into the branched chains. Meanwhile, the redshift of plasmon band is observed in the extinction spectra, which agrees well the growth at the tips of AgNTs and the decrease of the gaps between the adjacent nanotriangles. We also synthesize AgNT-Cu2O nanocomposites by using a photochemical method and find that the Cu2O nanoparticles preferably grow on the tips of AgNTs. The site-selective growth of Ag and Cu2O is interpreted by the local field concentration at the tips of AgNTs induced by surface plasmon resonance under light excitation.
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- 2017
46. Structural evolutions and hereditary characteristics of icosahedral nano-clusters formed in Mg70Zn30 alloys during rapid solidification processes
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Hairong Liu, Zean Tian, Li-li Zhou, Yongchao Liang, Zhao-Yang Hou, Hai-Tao Zhang, Quan Xie, Ping Peng, Yunfei Mo, and Rang-su Liu
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Multidisciplinary ,Materials science ,Icosahedral symmetry ,Alloy ,02 engineering and technology ,engineering.material ,021001 nanoscience & nanotechnology ,Microstructure ,01 natural sciences ,Article ,Molecular dynamics ,Pentagonal pyramid ,Nano clusters ,Chemical physics ,0103 physical sciences ,Atom ,engineering ,010306 general physics ,0210 nano-technology ,Index method - Abstract
To investigate the structural evolution and hereditary mechanism of icosahedral nano-clusters formed during rapid solidification, a molecular dynamics (MD) simulation study has been performed for a system consisting of 107 atoms of liquid Mg70Zn30 alloy. Adopting Honeycutt-Anderson (HA) bond-type index method and cluster type index method (CTIM-3) to analyse the microstructures in the system it is found that for all the nano-clusters including 2~8 icosahedral clusters in the system, there are 62 kinds of geometrical structures, and those can be classified, by the configurations of the central atoms of basic clusters they contained, into four types: chain-like, triangle-tailed, quadrilateral-tailed and pyramidal-tailed. The evolution of icosahedral nano-clusters can be conducted by perfect heredity and replacement heredity, and the perfect heredity emerges when temperature is slightly less than Tm then increase rapidly and far exceeds the replacement heredity at Tg; while for the replacement heredity, there are three major modes: replaced by triangle (3-atoms), quadrangle (4-atoms) and pentagonal pyramid (6-atoms), rather than by single atom step by step during rapid solidification processes.
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- 2017
47. Corrigendum: Long intergenic non-coding RNA 271 is predictive of a poorer prognosis of papillary thyroid cancer
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Ben Ma, Chuanpeng Dong, Tian Liao, Qinghai Ji, Yu Wang, Shuwen Yang, Li Zhou, and Duo Wen
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Adult ,Male ,Adolescent ,Down-Regulation ,Bioinformatics ,Article ,Papillary thyroid cancer ,Young Adult ,Intergenic region ,Databases, Genetic ,Medicine ,Humans ,Thyroid Neoplasms ,Aged ,Neoplasm Staging ,Aged, 80 and over ,Multidisciplinary ,business.industry ,Middle Aged ,Non-coding RNA ,medicine.disease ,Prognosis ,Corrigenda ,Carcinoma, Papillary ,Gene Expression Regulation, Neoplastic ,Thyroid Cancer, Papillary ,Lymphatic Metastasis ,Table (database) ,Female ,RNA, Long Noncoding ,business - Abstract
A number of long non-coding RNAs (lncRNAs) have been found to play critical roles in oncogenesis and tumor progression. We aimed to investigate whether lncRNAs could act as prognostic biomarkers for papillary thyroid cancer (PTC) that may assist us in evaluating disease status and prognosis for patients. We found 220 lncRNAs with expression alteration from the annotated 2773 lncRNAs approved by the HUGO gene nomenclature committee in The Cancer Genome Atlas (TCGA) dataset, of which FAM41C, CTBP1-AS2, LINC00271, HAR1A, LINC00310 and HAS2-AS1 were associated with recurrence. After adjusting classical clinicopathogical factors and BRAFV600E mutation, LINC00271 was found to be an independent risk factor for extrathyroidal extension, lymph node metastasis, advanced tumor stage III/IV and recurrence in multivariate analyses. Additionally, LINC00271 expression was significantly downregulated in PTCs versus adjacent normal tissues (P
- Published
- 2017
48. Chemical inhibition reveals differential requirements of signaling pathways in kras
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Chuan, Yan, Qiqi, Yang, Xiaojing, Huo, Hankun, Li, Li, Zhou, and Zhiyuan, Gong
- Subjects
Vascular Endothelial Growth Factor A ,Carcinoma, Hepatocellular ,Liver Neoplasms ,Genes, myc ,Zebrafish Proteins ,Article ,Animals, Genetically Modified ,Fibroblast Growth Factors ,Proto-Oncogene Proteins p21(ras) ,Disease Models, Animal ,Liver Neoplasms, Experimental ,Animals ,Zebrafish ,Signal Transduction - Abstract
Previously we have generated inducible liver tumor models by transgenic expression of an oncogene and robust tumorigenesis can be rapidly induced by activation of the oncogene in both juvenile and adult fish. In the present study, we aimed at chemical intervention of tumorigenesis for understanding molecular pathways of tumorigenesis and for potential development of a chemical screening tool for anti-cancer drug discovery. Thus, we evaluated the roles of several major signaling pathways in krasV12- or Myc-induced liver tumors by using several small molecule inhibitors: SU5402 and SU6668 for VEGF/FGF signaling; IWR1 and cardionogen 1 for Wnt signaling; and cyclopamine and Gant61 for Hedgehog signaling. Inhibition of VEGF/FGF signaling was found to deter both Myc- and krasV12-induced liver tumorigenesis while suppression of Wnt signaling relaxed only Myc- but not krasV12-induced liver tumorigenesis. Inhibiting Hedgehog signaling did not suppress either krasV12 or Myc-induced tumors. The suppression of liver tumorigenesis was accompanied with a decrease of cell proliferation, increase of apoptosis, distorted liver histology. Collectively, our observations suggested the requirement of VEGF/FGF signaling but not the hedgehog signaling in liver tumorigenesis in both transgenic fry. However, Wnt signaling appeared to be required for liver tumorigenesis only in Myc but not krasV12 transgenic zebrafish.
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- 2017
49. Correction: Corrigendum: Soybean-derived Bowman-Birk Inhibitor (BBI) Inhibits HIV Replication in Macrophages
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Ke Zhuang, Li Zhou, Jieliang Li, Deyin Guo, Wei Hou, Xu Wang, Tongcui Ma, Ming Sang, Run-Hong Zhou, and Wen-Zhe Ho
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0301 basic medicine ,03 medical and health sciences ,030104 developmental biology ,Multidisciplinary ,Bowman birk ,Replication (statistics) ,Human immunodeficiency virus (HIV) ,medicine ,Biology ,medicine.disease_cause ,Virology - Abstract
Scientific Reports 6: Article number: 34752; published online: 13 October 2016; updated: 23 December 2016
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- 2016
50. Copper-catalyzed Direct 2-Arylation of Benzoxazoles and Benzoimidazoles with Aryl Bromides and Cytotoxicity of Products
- Author
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Xiao-Xia Qu, Xin-Chuan Tian, Nan-Nan Jia, Ya-Nan Cao, Yun-Xin Yao, Feng Gao, Xian-Li Zhou, and Xing-Xiu Chen
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Bromides ,Cell Survival ,chemistry.chemical_element ,010402 general chemistry ,Crystallography, X-Ray ,01 natural sciences ,Catalysis ,Article ,chemistry.chemical_compound ,Cell Line, Tumor ,Molecule ,Humans ,Cytotoxicity ,Benzene ,Cell survival ,Benzoxazoles ,Multidisciplinary ,Molecular Structure ,010405 organic chemistry ,Aryl ,Imidazoles ,Hep G2 Cells ,HCT116 Cells ,Copper ,Combinatorial chemistry ,0104 chemical sciences ,chemistry ,Models, Chemical ,Copper catalyzed - Abstract
An efficient copper-catalyzed direct 2-arylation of benzoxazoles and benzoimidazoles with aryl bromides is presented. The CuI/PPh3-based catalyst promotes the installation of various aryl and heteroaryl groups through a C-H activation process in good to excellent yields. The cytotoxicity of obtained 2-aryl benzoxazoles (benzoimidazoles) was also evaluated and 1-methyl-2-(naphthalen-1-yl)benzoimidazole showed potential cytotoxicity.
- Published
- 2016
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