1. Crucial Role of NLRP3 Inflammasome in the Development of Peritoneal Dialysis-related Peritoneal Fibrosis
- Author
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Masafumi Takahashi, Yoshiyuki Morishita, Tetsu Akimoto, Emi Aizawa, Tadayoshi Karasawa, Daisuke Nagata, Ryo Kamata, Sachiko Watanabe, Hiroaki Kimura, Homare Ito, Takanori Komada, Erika Hishida, and Tadashi Kasahara
- Subjects
0301 basic medicine ,Inflammasomes ,medicine.medical_treatment ,Peritoneal dialysis ,Interleukin-1beta ,lcsh:Medicine ,Inflammation ,Kidney ,Article ,Proinflammatory cytokine ,Pathogenesis ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Peritoneum ,Fibrosis ,NLR Family, Pyrin Domain-Containing 3 Protein ,Human Umbilical Vein Endothelial Cells ,Leukocytes ,medicine ,Animals ,Humans ,lcsh:Science ,Peritoneal Fibrosis ,Mice, Knockout ,Multidisciplinary ,business.industry ,Macrophages ,lcsh:R ,Endothelial Cells ,Inflammasome ,Pyruvaldehyde ,medicine.disease ,CARD Signaling Adaptor Proteins ,Mice, Inbred C57BL ,Disease Models, Animal ,030104 developmental biology ,medicine.anatomical_structure ,Cancer research ,lcsh:Q ,Female ,medicine.symptom ,Reactive Oxygen Species ,business ,030217 neurology & neurosurgery ,medicine.drug - Abstract
Long-term peritoneal dialysis (PD) therapy leads to peritoneal inflammation and fibrosis. However, the mechanism underlying PD-related peritoneal inflammation and fibrosis remains unclear. NLRP3 inflammasome regulates the caspase-1-dependent release of interleukin-1β and mediates inflammation in various diseases. Here, we investigated the role of NLRP3 inflammasome in a murine model of PD-related peritoneal fibrosis induced by methylglyoxal (MGO). Inflammasome-related proteins were upregulated in the peritoneum of MGO-treated mice. MGO induced parietal and visceral peritoneal fibrosis in wild-type mice, which was significantly reduced in mice deficient in NLRP3, ASC, and interleukin-1β (IL-1β). ASC deficiency reduced the expression of inflammatory cytokines and fibrotic factors, and the infiltration of macrophages. However, myeloid cell-specific ASC deficiency failed to inhibit MGO-induced peritoneal fibrosis. MGO caused hemorrhagic ascites, fibrin deposition, and plasminogen activator inhibitor-1 upregulation, but all of these manifestations were inhibited by ASC deficiency. Furthermore, in vitro experiments showed that MGO induced cell death via the generation of reactive oxygen species in vascular endothelial cells, which was inhibited by ASC deficiency. Our results showed that endothelial NLRP3 inflammasome contributes to PD-related peritoneal inflammation and fibrosis, and provide new insights into the mechanisms underlying the pathogenesis of this disorder.
- Published
- 2019