8 results on '"Thi Bui"'
Search Results
2. Nasal-spraying Bacillus spores as an effective symptomatic treatment for children with acute respiratory syncytial virus infection
- Author
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Dien Minh Tran, Tu Thanh Tran, Thuy Thi Bich Phung, Huyen Thi Bui, Phuc Thanh Thi Nguyen, Tam Thi Vu, Nga Thi Phuong Ngo, Mai Thi Nguyen, Anh Hoa Nguyen, and Anh Thi Van Nguyen
- Subjects
Spores, Bacterial ,Multidisciplinary ,Child, Preschool ,Respiratory Syncytial Virus, Human ,Humans ,Bacillus ,Nasal Sprays ,Respiratory Syncytial Virus Infections ,Child ,Respiratory Tract Infections - Abstract
Respiratory syncytial virus (RSV) is a leading cause of Acute Respiratory Tract Infections (ARTIs) in young children. However, there is currently no vaccine or treatment available for children. Here, we demonstrated that nasal-spraying probiotics containing 5 billion of Bacillus spores (LiveSpo Navax) is an effective symptomatic treatment in a 6-day randomized controlled clinical study for RSV-infected children (n = 40–46/group). Navax treatment resulted in 1-day faster recovery-time and 10–50% better efficacy in relieving ARTI symptoms. At day 3, RSV load and level of pro-inflammatory cytokines in nasopharyngeal samples was reduced by 630 folds and 2.7–12.7 folds respectively. This showed 53-fold and 1.8–3.6-fold more effective than those in the control-standard of care-group. In summary, nasal-spraying Bacillus spores can rapidly and effectively relieve symptoms of RSV-induced ARTIs while exhibit strong impacts in reducing viral load and inflammation. Our nasal-spraying probiotics may provide a basis for simple-to-use, low-cost, and effective treatment against viral infection in general.
- Published
- 2021
3. Clinical evaluation of rapid fluorescent diagnostic immunochromatographic test for influenza A virus (H1N1)
- Author
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Anh Viet Thi Nguyen, Seung-Taek Yu, Cuc Thi Bui, Thuy Tien Thi Trinh, Do Thi Hoang Kim, and Seon-Ju Yeo
- Subjects
0301 basic medicine ,Adult ,Male ,medicine.medical_specialty ,Adolescent ,Point-of-care testing ,030106 microbiology ,Immunochromatographic test ,Fluorescent Antibody Technique ,lcsh:Medicine ,medicine.disease_cause ,Gastroenterology ,Article ,03 medical and health sciences ,0302 clinical medicine ,McNemar's test ,Influenza A Virus, H1N1 Subtype ,Limit of Detection ,Internal medicine ,Influenza, Human ,Influenza A virus ,Medicine ,Humans ,H1n1 infection ,030212 general & internal medicine ,Child ,lcsh:Science ,Multidisciplinary ,business.industry ,lcsh:R ,Confidence interval ,Child, Preschool ,Female ,lcsh:Q ,business ,Clinical evaluation ,Kappa - Abstract
Rapid diagnostic tests (RDTs) have been developed to detect influenza A virus for the swift diagnosis and management of patients. However, despite the simplicity and convenience, the low sensitivity of RDTs remains a limitation for their use in point of care testing (POCT). In this study, we developed a rapid fluorescent immunochromatographic strip test (FICT) and the performance of FICT was confirmed by the real-time reverse transcription-polymerase chain reaction (rRT-PCR) of H1N1, compared with that of RDT. The limit of detection (LOD) of FICT was improved by 16-fold compared to RDT. FICT showed 85.29% sensitivity (29/34) (95% Confidence Interval [95% CI]: 68.94 to 95.05), 100% specificity (26/26) (95% CI: 86.78 to 100.00), and a strong correlation (kappa; 0. 92) compared with rRT-PCR (20 ≤ Ct ≤ 36). In contrast, RDT (Standard Diagnostics [SD] BIOLINE Influenza Ag A/ B/ A(H1N1) Pandemic) showed 55.88% sensitivity (19/34) (95% CI: 37.87 to 72.82), 100% specificity (26/26) (95% CI: 77.07 to 100), and had a fair correlation with rRT-PCR (kappa; 0. 75). FICT had better sensitivity than RDT (P
- Published
- 2018
4. Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles
- Author
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Nguyen Thi Viet Anh, Duong Tuan Bao, Trinh Thi Thuy Tien, Nguyen Thi Phuong Linh, Ho-Joon Shin, Cuc Thi Bui, Seon-Ju Yeo, Hae-Jin Sohn, Hyun Park, Chom-Kyu Chong, Do Thi Hoang Kim, and Ga-Eun Seo
- Subjects
0301 basic medicine ,Hemagglutination ,medicine.drug_class ,Science ,Fluoroimmunoassay ,Metal Nanoparticles ,Hemagglutinin (influenza) ,Hemagglutinin Glycoproteins, Influenza Virus ,Antibodies, Viral ,Influenza A Virus, H7N9 Subtype ,medicine.disease_cause ,Monoclonal antibody ,Immunofluorescence ,Sensitivity and Specificity ,Article ,Virus ,Madin Darby Canine Kidney Cells ,03 medical and health sciences ,Dogs ,Europium ,Orthomyxoviridae Infections ,Limit of Detection ,Lysis buffer ,medicine ,Animals ,Multidisciplinary ,biology ,medicine.diagnostic_test ,Antibodies, Monoclonal ,Virology ,Molecular biology ,Recombinant Proteins ,Influenza A virus subtype H5N1 ,030104 developmental biology ,biology.protein ,Medicine ,Reagent Kits, Diagnostic ,Antibody - Abstract
The development of a sensitive and rapid diagnostic test is needed for early detection of avian influenza (AI) H7 subtype. In this study, novel monoclonal antibodies (mAbs) against influenza A H7N9 recombinant hemagglutinin (rHA)1 were developed and applied to a Europium nanoparticle–based rapid fluorescent immunochromatographic strip test (FICT) to improve the sensitivity of the rapid diagnostic system. Two antibodies (2F4 and 6D7) exhibited H7 subtype specificity in a dot-FICT assay by optimization of the conjugate and the pH of the lysis buffer. The subtype specificity was confirmed by an immunofluorescence assay and Western blot analysis. The limit of detection of the FICT employing novel mAbs 31 ng/mL for H7N9 rHA1 and 40 hemagglutination units/mL for H7 subtype virus. Sensitivity was improved 25-fold using Europium as confirmed by comparison of colloidal gold-based rapid diagnostic kit using the 2F4 and 6D7 mAbs.
- Published
- 2017
5. Community-based prevalence and associated factors of sarcopenia in the Vietnamese elderly
- Author
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Lan-Anh Thi Pham, Binh Thanh Nguyen, Dao Tieu Huynh, Binh-Minh Le Thi Nguyen, Phuong-Anh Nhat Tran, Tam Van Vo, Hy-Han Thi Bui, and Truc Thanh Thai
- Subjects
Medicine ,Science - Abstract
Abstract Sarcopenia, a condition characterized by muscle mass decline, is one of the leading health problems in the elderly. This study determined the rate of sarcopenia according to criteria by Asian Working Group for Sarcopenia (AWGS) and related factors in elderly people. A community-based cross-sectional study was conducted in 632 people aged 60 years or over in Ho Chi Minh City. Data were collected through a predefined questionnaire and direct measurement. Sarcopenia was identified based on the Inbody 770 machine and AWGS criteria. The prevalence of sarcopenia was 32.0%. Participants with advanced age, low education, unemployment, low level of family economics and frailty were more likely to have sarcopenia. Among these, frailty had the highest impact on sarcopenia, with significantly higher odds of having sarcopenia found in participants with pre-frailty (OR = 4.80, 95% CI 2.75–8.38, p
- Published
- 2024
- Full Text
- View/download PDF
6. Efficient symptomatic treatment and viral load reduction for children with influenza virus infection by nasal-spraying Bacillus spore probiotics
- Author
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Tu Thanh Tran, Thuy Thi Bich Phung, Dien Minh Tran, Huyen Thi Bui, Phuc Thanh Thi Nguyen, Tam Thi Vu, Nga Thi Phuong Ngo, Mai Thi Nguyen, Anh Hoa Nguyen, and Anh Thi Van Nguyen
- Subjects
Medicine ,Science - Abstract
Abstract Influenza virus is a main cause of acute respiratory tract infections (ARTIs) in children. This is the first double-blind, randomized, and controlled clinical trial examining the efficacy of nasal-spraying probiotic LiveSpo Navax, which contains 5 billion of Bacillus subtilis and B. clausii spores in 5 mL, in supporting treatment of influenza viral infection in pediatric patients. We found that the nasal-spraying Bacillus spores significantly shortened the recovery period and overall treatment by 2 days and increased treatment effectiveness by 58% in resolving all ARTIs’ symptoms. At day 2, the concentrations of influenza virus and co-infected bacteria were reduced by 417 and 1152 folds. Additionally, the levels of pro-inflammatory cytokines IL-8, TNF-α, and IL-6 in nasopharyngeal samples were reduced by 1.1, 3.7, and 53.9 folds, respectively. Compared to the standard control group, treatment regimen with LiveSpo Navax demonstrated significantly greater effectiveness, resulting in 26-fold reduction in viral load, 65-fold reduction in bacterial concentration, and 1.1–9.5-fold decrease in cytokine levels. Overall, nasal-spraying Bacillus spores can support the symptomatic treatment of influenza virus-induced ARTIs quickly, efficiently and could be used as a cost-effective supportive treatment for respiratory viral infection in general. Clinical trial registration no: NCT05378022 on 17/05/2022.
- Published
- 2023
- Full Text
- View/download PDF
7. Nasal-spraying Bacillus spores as an effective symptomatic treatment for children with acute respiratory syncytial virus infection
- Author
-
Dien Minh Tran, Tu Thanh Tran, Thuy Thi Bich Phung, Huyen Thi Bui, Phuc Thanh Thi Nguyen, Tam Thi Vu, Nga Thi Phuong Ngo, Mai Thi Nguyen, Anh Hoa Nguyen, and Anh Thi Van Nguyen
- Subjects
Medicine ,Science - Abstract
Abstract Respiratory syncytial virus (RSV) is a leading cause of Acute Respiratory Tract Infections (ARTIs) in young children. However, there is currently no vaccine or treatment available for children. Here, we demonstrated that nasal-spraying probiotics containing 5 billion of Bacillus spores (LiveSpo Navax) is an effective symptomatic treatment in a 6-day randomized controlled clinical study for RSV-infected children (n = 40–46/group). Navax treatment resulted in 1-day faster recovery-time and 10–50% better efficacy in relieving ARTI symptoms. At day 3, RSV load and level of pro-inflammatory cytokines in nasopharyngeal samples was reduced by 630 folds and 2.7–12.7 folds respectively. This showed 53-fold and 1.8–3.6-fold more effective than those in the control-standard of care-group. In summary, nasal-spraying Bacillus spores can rapidly and effectively relieve symptoms of RSV-induced ARTIs while exhibit strong impacts in reducing viral load and inflammation. Our nasal-spraying probiotics may provide a basis for simple-to-use, low-cost, and effective treatment against viral infection in general.
- Published
- 2022
- Full Text
- View/download PDF
8. Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles
- Author
-
Seon-Ju Yeo, Duong Tuan Bao, Ga-Eun Seo, Cuc Thi Bui, Do Thi Hoang Kim, Nguyen Thi Viet Anh, Trinh Thi Thuy Tien, Nguyen Thi Phuong Linh, Hae-Jin Sohn, Chom-Kyu Chong, Ho-Joon Shin, and Hyun Park
- Subjects
Medicine ,Science - Abstract
Abstract The development of a sensitive and rapid diagnostic test is needed for early detection of avian influenza (AI) H7 subtype. In this study, novel monoclonal antibodies (mAbs) against influenza A H7N9 recombinant hemagglutinin (rHA)1 were developed and applied to a Europium nanoparticle–based rapid fluorescent immunochromatographic strip test (FICT) to improve the sensitivity of the rapid diagnostic system. Two antibodies (2F4 and 6D7) exhibited H7 subtype specificity in a dot-FICT assay by optimization of the conjugate and the pH of the lysis buffer. The subtype specificity was confirmed by an immunofluorescence assay and Western blot analysis. The limit of detection of the FICT employing novel mAbs 31 ng/mL for H7N9 rHA1 and 40 hemagglutination units/mL for H7 subtype virus. Sensitivity was improved 25-fold using Europium as confirmed by comparison of colloidal gold-based rapid diagnostic kit using the 2F4 and 6D7 mAbs.
- Published
- 2017
- Full Text
- View/download PDF
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