32 results on '"Yogo, A."'
Search Results
2. Post-extubation dysphagia in pediatric trauma patients: a single-center case-series study
- Author
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Naoki Yogo, Takeru Abe, Kyoko Kano, Yuichiro Muto, Sachi Kiyonaga, and Katsuki Hirai
- Subjects
Medicine ,Science - Abstract
Abstract We aimed to investigate whether ventilator support time influences the occurrence of dysphagia in pediatric trauma patients. This case-series study was conducted in a single pediatric emergency and critical care center from April 2012 to March 2022. Trauma patients aged
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- 2024
- Full Text
- View/download PDF
3. High-performance glass filters for capturing and culturing circulating tumor cells and cancer-associated fibroblasts
- Author
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Tanaka, Hiromasa, Iwata, Daijiro, Shibata, Yuki, Hase, Tetsunari, Onoshima, Daisuke, Yogo, Naoyuki, Shibata, Hirofumi, Sato, Mitsuo, Ishikawa, Kenji, Nagasawa, Ikuo, Hasegawa, Yoshinori, Ishii, Makoto, Baba, Yoshinobu, and Hori, Masaru
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- 2023
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4. High-performance glass filters for capturing and culturing circulating tumor cells and cancer-associated fibroblasts
- Author
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Hiromasa Tanaka, Daijiro Iwata, Yuki Shibata, Tetsunari Hase, Daisuke Onoshima, Naoyuki Yogo, Hirofumi Shibata, Mitsuo Sato, Kenji Ishikawa, Ikuo Nagasawa, Yoshinori Hasegawa, Makoto Ishii, Yoshinobu Baba, and Masaru Hori
- Subjects
Medicine ,Science - Abstract
Abstract Various liquid biopsy methods have been developed for the non-invasive and early detection of diseases. In particular, the detection of circulating tumor cells (CTCs) and cancer-associated fibroblasts (CAFs) in blood has been receiving a great deal of attention. We have been developing systems and materials to facilitate such liquid biopsies. In this study, we further developed glass filters (with various patterns of holes, pitches, and non-adhesive coating) that can capture CTCs, but not white blood cells. We optimized the glass filters to capture CTCs, and demonstrated that they could be used to detect CTCs from lung cancer patients. We also used the optimized glass filters for detecting CAFs. Additionally, we further developed a system for visualizing the captured cells on the glass filters. Finally, we demonstrated that we could directly culture the captured cells on the glass filters. Based on these results, our high-performance glass filters appear to be useful for capturing and culturing CTCs and CAFs for further examinations.
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- 2023
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5. Super-strong magnetic field-dominated ion beam dynamics in focusing plasma devices
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A. Morace, Y. Abe, J. J. Honrubia, N. Iwata, Y. Arikawa, Y. Nakata, T. Johzaki, A. Yogo, Y. Sentoku, K. Mima, T. Ma, D. Mariscal, H. Sakagami, T. Norimatsu, K. Tsubakimoto, J. Kawanaka, S. Tokita, N. Miyanaga, H. Shiraga, Y. Sakawa, M. Nakai, H. Azechi, S. Fujioka, and R. Kodama
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Medicine ,Science - Abstract
Abstract High energy density physics is the field of physics dedicated to the study of matter and plasmas in extreme conditions of temperature, densities and pressures. It encompasses multiple disciplines such as material science, planetary science, laboratory and astrophysical plasma science. For the latter, high energy density states can be accompanied by extreme radiation environments and super-strong magnetic fields. The creation of high energy density states in the laboratory consists in concentrating/depositing large amounts of energy in a reduced mass, typically solid material sample or dense plasma, over a time shorter than the typical timescales of heat conduction and hydrodynamic expansion. Laser-generated, high current–density ion beams constitute an important tool for the creation of high energy density states in the laboratory. Focusing plasma devices, such as cone-targets are necessary in order to focus and direct these intense beams towards the heating sample or dense plasma, while protecting the proton generation foil from the harsh environments typical of an integrated high-power laser experiment. A full understanding of the ion beam dynamics in focusing devices is therefore necessary in order to properly design and interpret the numerous experiments in the field. In this work, we report a detailed investigation of large-scale, kilojoule-class laser-generated ion beam dynamics in focusing devices and we demonstrate that high-brilliance ion beams compress magnetic fields to amplitudes exceeding tens of kilo-Tesla, which in turn play a dominant role in the focusing process, resulting either in a worsening or enhancement of focusing capabilities depending on the target geometry.
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- 2022
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6. Super-strong magnetic field-dominated ion beam dynamics in focusing plasma devices
- Author
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Morace, A., Abe, Y., Honrubia, J. J., Iwata, N., Arikawa, Y., Nakata, Y., Johzaki, T., Yogo, A., Sentoku, Y., Mima, K., Ma, T., Mariscal, D., Sakagami, H., Norimatsu, T., Tsubakimoto, K., Kawanaka, J., Tokita, S., Miyanaga, N., Shiraga, H., Sakawa, Y., Nakai, M., Azechi, H., Fujioka, S., and Kodama, R.
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- 2022
- Full Text
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7. Time–frequency analysis of serum with proton nuclear magnetic resonance for diagnosis of pancreatic cancer
- Author
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Asahi Sato, Toshihiko Masui, Akitada Yogo, Takashi Ito, Keiko Hirakawa, Yoshimasa Kanawaku, Kaoru Koike, and Shinji Uemoto
- Subjects
Medicine ,Science - Abstract
Abstract Although serum markers such as carcinoembryonic antigen (CEA) and carbohydrate antigen (CA19-9) have been widely used in screening for pancreatic cancer (PC), their sensitivity and specificity are unsatisfactory. Recently, a novel tool of analyzing serum using the short-time Fourier transform (STFT) of free induction decays (FIDs) obtained by 1H-NMR has been introduced. We for the first time evaluated the utility of this technology as a diagnostic tool for PC. Serum was obtained from PC patients before starting any treatments. Samples taken from individuals with benign diseases or donors for liver transplantation were obtained as controls. Serum samples from both groups underwent 1H-NMR and STFT of FIDs. STFT data were analyzed by partial least squares discriminant analysis (PLS-DA) to clarify whether differences were apparent between groups. As a result, PLS-DA score plots indicated that STFT of FIDs enabled effective classification of groups with and without PC. Additionally, in a subgroup of PC, long-term survivors (≥ 2 years) could be discriminated from short-term survivors (
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- 2020
- Full Text
- View/download PDF
8. Author Correction: Proof-of-principle experiment for laser-driven cold neutron source
- Author
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Mirfayzi, S. R., Yogo, A., Lan, Z., Ishimoto, T., Iwamoto, A., Nagata, M., Nakai, M., Arikawa, Y., Abe, Y., Golovin, D., Honoki, Y., Mori, T., Okamoto, K., Shokita, S., Neely, D., Fujioka, S., Mima, K., Nishimura, H., Kar, S., and Kodama, R.
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- 2021
- Full Text
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9. Author Correction: Proof-of-principle experiment for laser-driven cold neutron source
- Author
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S. R. Mirfayzi, A. Yogo, Z. Lan, T. Ishimoto, A. Iwamoto, M. Nagata, M. Nakai, Y. Arikawa, Y. Abe, D. Golovin, Y. Honoki, T. Mori, K. Okamoto, S. Shokita, D. Neely, S. Fujioka, K. Mima, H. Nishimura, S. Kar, and R. Kodama
- Subjects
Medicine ,Science - Abstract
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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- 2021
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10. Proof-of-principle experiment for laser-driven cold neutron source
- Author
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Mirfayzi, S. R., Yogo, A., Lan, Z., Ishimoto, T., Iwamoto, A., Nagata, M., Nakai, M., Arikawa, Y., Abe, Y., Golovin, D., Honoki, Y., Mori, T., Okamoto, K., Shokita, S., Neely, D., Fujioka, S., Mima, K., Nishimura, H., Kar, S., and Kodama, R.
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- 2020
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11. Time–frequency analysis of serum with proton nuclear magnetic resonance for diagnosis of pancreatic cancer
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Sato, Asahi, Masui, Toshihiko, Yogo, Akitada, Ito, Takashi, Hirakawa, Keiko, Kanawaku, Yoshimasa, Koike, Kaoru, and Uemoto, Shinji
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- 2020
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12. Dlec1 is required for spermatogenesis and male fertility in mice
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Okitsu, Yu, Nagano, Mamoru, Yamagata, Takahiro, Ito, Chizuru, Toshimori, Kiyotaka, Dohra, Hideo, Fujii, Wataru, and Yogo, Keiichiro
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- 2020
- Full Text
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13. Imaging Cherenkov emission for quality assurance of high-dose-rate brachytherapy
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Yogo, Katsunori, Matsushita, Akihiro, Tatsuno, Yuya, Shimo, Takahiro, Hirota, Seiko, Nozawa, Marika, Ozawa, Shuichi, Ishiyama, Hiromichi, Yasuda, Hiroshi, Nagata, Yasushi, and Hayakawa, Kazushige
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- 2020
- Full Text
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14. Electromagnetic Burst Generation during Annihilation of Magnetic Field in Relativistic Laser-Plasma Interaction
- Author
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Gu, Y. J., Pegoraro, F., Sasorov, P. V., Golovin, D., Yogo, A., Korn, G., and Bulanov, S. V.
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- 2019
- Full Text
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15. Control of unsteady laser-produced plasma-flow with a multiple-coil magnetic nozzle
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Taichi Morita, Masafumi Edamoto, Satoshi Miura, Atsushi Sunahara, Naoya Saito, Yutaro Itadani, Tomihiko Kojima, Yoshitaka Mori, Tomoyuki Johzaki, Yoshihiro Kajimura, Shinsuke Fujioka, Akifumi Yogo, Hiroaki Nishimura, Hideki Nakashima, and Naoji Yamamoto
- Subjects
Medicine ,Science - Abstract
Abstract We report an experimental demonstration of controlling plasma flow direction with a magnetic nozzle consisting of multiple coils. Four coils are controlled separately to form an asymmetric magnetic field to change the direction of laser-produced plasma flow. The ablation plasma deforms the topology of the external magnetic field, forming a magnetic cavity inside and compressing the field outside. The compressed magnetic field pushes the plasma via the Lorentz force on a diamagnetic current: j × B in a certain direction, depending on the magnetic field configuration. Plasma and magnetic field structure formations depending on the initial magnetic field were simultaneously measured with a self-emission gated optical imager and B-dot probe, respectively, and the probe measurement clearly shows the difference of plasma expansion direction between symmetric and asymmetric initial magnetic fields. The combination of two-dimensional radiation hydrodynamic and three-dimensional hybrid simulations shows the control of the deflection angle with different number of coils, forming a plasma structure similar to that observed in the experiment.
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- 2017
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16. Mesoscopic Multimodal Imaging Provides New Insight to Tumor Tissue Evaluation: An Example of Macrophage Imaging of Hepatic Tumor using Organosilica Nanoparticles
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Michihiro Nakamura, Koichiro Hayashi, Hitoshi Kubo, Masafumi Harada, Keisuke Izumi, Yoshihiro Tsuruo, and Toshinobu Yogo
- Subjects
Medicine ,Science - Abstract
Abstract Multimodal imaging using novel multifunctional nanoparticles provides new approach to biomedical field. Thiol-organosilica nanoparticles containing iron oxide magnetic nanoparticles (MNPs) and rhodamine B (thiol OS-MNP/Rho) were applied to multimodal imaging of hepatic tumor of Long−Evans Cinnamon (LEC) rat. The magnetic resonance imaging (MRI) of LEC rats revealed tumors in the liver clearly and semi-quantitatively due to a labeling of macrophages in liver. The fluorescent imaging (FI) showed abnormal fluorescent patterns of the liver at the mesoscopic level that was between macroscopic and microscopic level. We performed correlation analysis between optical imaging including FI and MRI. We found that the labeled macrophages located specific area in the tumor tissue and influenced the tumor size on MRI. In addition histological observation showed the labeled macrophages related specific tissue in the pathological region. We demonstrated a new approach to evaluate tumor tissue at the macroscopic and microscopic level as well as mesoscopic level using multimodal imaging.
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- 2017
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17. Newly developed Laboratory-based Size exclusion chromatography Small-angle x-ray scattering System (La-SSS)
- Author
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Inoue, Rintaro, Nakagawa, Tatsuo, Morishima, Ken, Sato, Nobuhiro, Okuda, Aya, Urade, Reiko, Yogo, Rina, Yanaka, Saeko, Yagi-Utsumi, Maho, Kato, Koichi, Omoto, Kazuki, Ito, Kazuki, and Sugiyama, Masaaki
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- 2019
- Full Text
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18. The Fab portion of immunoglobulin G contributes to its binding to Fcγ receptor III
- Author
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Yogo, Rina, Yamaguchi, Yuki, Watanabe, Hiroki, Yagi, Hirokazu, Satoh, Tadashi, Nakanishi, Mahito, Onitsuka, Masayoshi, Omasa, Takeshi, Shimada, Mari, Maruno, Takahiro, Torisu, Tetsuo, Watanabe, Shio, Higo, Daisuke, Uchihashi, Takayuki, Yanaka, Saeko, Uchiyama, Susumu, and Kato, Koichi
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- 2019
- Full Text
- View/download PDF
19. Proof-of-principle experiment for laser-driven cold neutron source
- Author
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Yasuhiro Abe, S. R. Mirfayzi, M. Nakai, Zechen Lan, R. Kodama, D. Neely, K. Mima, Takuya Ishimoto, Hiroaki Nishimura, Akifumi Iwamoto, Shinsuke Fujioka, D. Golovin, T. Mori, Yuki Honoki, Kiyoko Okamoto, S. Shokita, Yasunobu Arikawa, M. Nagata, S. Kar, and Akifumi Yogo
- Subjects
Brightness ,Flux ,lcsh:Medicine ,639/766/1960/1135 ,01 natural sciences ,Article ,010305 fluids & plasmas ,law.invention ,Nuclear physics ,law ,Neutron flux ,0103 physical sciences ,Neutron ,010306 general physics ,lcsh:Science ,Physics ,Multidisciplinary ,lcsh:R ,article ,Laser-produced plasmas ,Laser ,Beamline ,Temporal resolution ,639/766/1960/1137 ,Neutron source ,lcsh:Q ,Plasma-based accelerators - Abstract
The scientific and technical advances continue to support novel discoveries by allowing scientists to acquire new insights into the structure and properties of matter using new tools and sources. Notably, neutrons are among the most valuable sources in providing such a capability. At the Institute of Laser Engineering, Osaka, the first steps are taken towards the development of a table-top laser-driven neutron source, capable of producing a wide range of energies with high brightness and temporal resolution. By employing a pure hydrogen moderator, maintained at cryogenic temperature, a cold neutron ($$\le 25\hbox { meV}$$ ≤ 25 meV ) flux of $$\sim 2\times 10^3\hbox { n/cm}^2$$ ∼ 2 × 10 3 n/cm 2 /pulse was measured at the proximity of the moderator exit surface. The beam duration of hundreds of ns to tens of $$\upmu \hbox {s}$$ μ s is evaluated for neutron energies ranging from 100s keV down to meV via Monte-Carlo techniques. Presently, with the upcoming J-EPoCH high repetition rate laser at Osaka University, a cold neutron flux in orders of $$\sim 1\times 10^{9}\hbox { n/cm}^2/\hbox {s}$$ ∼ 1 × 10 9 n/cm 2 / s is expected to be delivered at the moderator in a compact beamline.
- Published
- 2020
20. Dlec1 is required for spermatogenesis and male fertility in mice
- Author
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Takahiro Yamagata, Hideo Dohra, Wataru Fujii, Keiichiro Yogo, Mamoru Nagano, Kiyotaka Toshimori, Yu Okitsu, and Chizuru Ito
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Male ,Reproductive biology ,lcsh:Medicine ,Biology ,Ion Channels ,Article ,Male infertility ,Mice ,Tubulin ,Intraflagellar transport ,medicine ,Animals ,Humans ,Spermatogenesis ,lcsh:Science ,Infertility, Male ,Multidisciplinary ,Tumor Suppressor Proteins ,Cilium ,HEK 293 cells ,lcsh:R ,medicine.disease ,Spermatozoa ,Phenotype ,Sperm ,Cell biology ,HEK293 Cells ,A549 Cells ,Organ Specificity ,Differentiation ,Adenocarcinoma ,lcsh:Q ,CRISPR-Cas Systems ,Gene Deletion - Abstract
Deleted in lung and esophageal cancer 1 (DLEC1) is a tumour suppressor gene that is downregulated in various cancers in humans; however, the physiological and molecular functions of DLEC1 are still unclear. This study investigated the critical role of Dlec1 in spermatogenesis and male fertility in mice. Dlec1 was significantly expressed in testes, with dominant expression in germ cells. We disrupted Dlec1 in mice and analysed its function in spermatogenesis and male fertility. Dlec1 deletion caused male infertility due to impaired spermatogenesis. Spermatogenesis progressed normally to step 8 spermatids in Dlec1−/− mice, but in elongating spermatids, we observed head deformation, a shortened tail, and abnormal manchette organization. These phenotypes were similar to those of various intraflagellar transport (IFT)-associated gene-deficient sperm. In addition, DLEC1 interacted with tailless complex polypeptide 1 ring complex (TRiC) and Bardet–Biedl Syndrome (BBS) protein complex subunits, as well as α- and β-tubulin. DLEC1 expression also enhanced primary cilia formation and cilia length in A549 lung adenocarcinoma cells. These findings suggest that DLEC1 is a possible regulator of IFT and plays an essential role in sperm head and tail formation in mice.
- Published
- 2020
21. Time–frequency analysis of serum with proton nuclear magnetic resonance for diagnosis of pancreatic cancer
- Author
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Keiko Hirakawa, Asahi Sato, Kaoru Koike, Toshihiko Masui, Shinji Uemoto, Akitada Yogo, Takashi Ito, and Yoshimasa Kanawaku
- Subjects
0301 basic medicine ,Adult ,Male ,medicine.medical_specialty ,CA-19-9 Antigen ,Science ,medicine.medical_treatment ,Liver transplantation ,Gastroenterology ,Article ,03 medical and health sciences ,0302 clinical medicine ,Text mining ,Carcinoembryonic antigen ,Internal medicine ,Pancreatic cancer ,Medicine ,Humans ,Nuclear Magnetic Resonance, Biomolecular ,Cancer ,Aged ,Aged, 80 and over ,Multidisciplinary ,biology ,business.industry ,Diagnostic markers ,Middle Aged ,Serum samples ,medicine.disease ,Carcinoembryonic Antigen ,Pancreatic Neoplasms ,030104 developmental biology ,030220 oncology & carcinogenesis ,Proton NMR ,biology.protein ,Female ,business ,Carbohydrate antigen ,Biomarkers ,Serum markers - Abstract
Although serum markers such as carcinoembryonic antigen (CEA) and carbohydrate antigen (CA19-9) have been widely used in screening for pancreatic cancer (PC), their sensitivity and specificity are unsatisfactory. Recently, a novel tool of analyzing serum using the short-time Fourier transform (STFT) of free induction decays (FIDs) obtained by 1H-NMR has been introduced. We for the first time evaluated the utility of this technology as a diagnostic tool for PC. Serum was obtained from PC patients before starting any treatments. Samples taken from individuals with benign diseases or donors for liver transplantation were obtained as controls. Serum samples from both groups underwent 1H-NMR and STFT of FIDs. STFT data were analyzed by partial least squares discriminant analysis (PLS-DA) to clarify whether differences were apparent between groups. As a result, PLS-DA score plots indicated that STFT of FIDs enabled effective classification of groups with and without PC. Additionally, in a subgroup of PC, long-term survivors (≥ 2 years) could be discriminated from short-term survivors (1H-NMR have a potential to be a diagnostic and prognostic tool of PC.
- Published
- 2020
22. Imaging Cherenkov emission for quality assurance of high-dose-rate brachytherapy
- Author
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Hiromichi Ishiyama, Marika Nozawa, Yasushi Nagata, Akihiro Matsushita, Kazushige Hayakawa, Katsunori Yogo, Yuya Tatsuno, Seiko Hirota, Hiroshi Yasuda, Shuichi Ozawa, and Takahiro Shimo
- Subjects
Materials science ,medicine.medical_treatment ,Astrophysics::High Energy Astrophysical Phenomena ,Monte Carlo method ,Brachytherapy ,Physics::Medical Physics ,Quantum physics ,lcsh:Medicine ,Radiation ,Article ,030218 nuclear medicine & medical imaging ,Techniques and instrumentation ,03 medical and health sciences ,0302 clinical medicine ,Optics ,medicine ,lcsh:Science ,Image resolution ,Cherenkov radiation ,Multidisciplinary ,business.industry ,lcsh:R ,High-Dose Rate Brachytherapy ,Applied physics ,Optics and photonics ,030220 oncology & carcinogenesis ,Light emission ,lcsh:Q ,business ,Quality assurance - Abstract
With advances in high-dose-rate (HDR) brachytherapy, the importance of quality assurance (QA) is increasing to ensure safe delivery of the treatment by measuring dose distribution and positioning the source with much closer intervals for highly active sources. However, conventional QA is time-consuming, involving the use of several different measurement tools. Here, we developed simple QA method for HDR brachytherapy based on the imaging of Cherenkov emission and evaluated its performance. Light emission from pure water irradiated by an 192Ir γ-ray source was captured using a charge-coupled device camera. Monte Carlo calculations showed that the observed light was primarily Cherenkov emissions produced by Compton-scattered electrons from the γ-rays. The uncorrected Cherenkov light distribution, which was 5% on average except near the source (within 7 mm from the centre), agreed with the dose distribution calculated using the treatment planning system. The accuracy was attributed to isotropic radiation and short-range Compton electrons. The source positional interval, as measured from the light images, was comparable to the expected intervals, yielding spatial resolution similar to that permitted by conventional film measurements. The method should be highly suitable for quick and easy QA investigations of HDR brachytherapy as it allows simultaneous measurements of dose distribution, source strength, and source position using a single image.
- Published
- 2020
23. Electromagnetic Burst Generation during Annihilation of Magnetic Field in Relativistic Laser-Plasma Interaction
- Author
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Akifumi Yogo, Georg Korn, D. Golovin, Sergei V. Bulanov, Yanjun Gu, Pavel V. Sasorov, and Francesco Pegoraro
- Subjects
FOS: Physical sciences ,lcsh:Medicine ,01 natural sciences ,Article ,law.invention ,relativistic plasma reconnection ,Current sheet ,Physics::Plasma Physics ,law ,Electric field ,0103 physical sciences ,lcsh:Science ,010306 general physics ,010303 astronomy & astrophysics ,Physics ,Multidisciplinary ,Annihilation ,lcsh:R ,Magnetic reconnection ,Laser-produced plasmas ,Plasma ,Laser ,Physics - Plasma Physics ,Charged particle ,Magnetic field ,Plasma Physics (physics.plasm-ph) ,Physics::Space Physics ,lcsh:Q ,Atomic physics ,Plasma-based accelerators - Abstract
We present the results of theoretical studies of formation and evolution of the current sheet in a colliosionless plasma during magnetic reconnection in relativistic limit. Relativistic magnetic reconnection is driven by parallel laser pulses interacting with underdense plasma target. Annihilation of laser created magnetic field of opposite polarity generates strong non-stationary electric field formed in between the region with opposite polarity magnetic field accelerating charged particles within the current sheet. This laser-plasma target configuration is discussed in regard with the laboratory modeling of charged particle acceleration and gamma flash generation in astrophysics. We present the results of 3-dimensional kinetic simulations and theoretical studies on the formation and evolution of the current sheet in a collisionless plasma during magnetic field annihilation in the ultra-relativistic limit. Annihilation of oppositively directed magnetic fields driven by two laser pulses interacting with underdense plasma target is accompanied by an electromagnetic burst generation. The induced strong non-stationary longitudinal electric field accelerates charged particles within the current sheet. Properties of the laser-plasma target configuration are discussed in the context of the laboratory modeling for charged particle acceleration and gamma flash generation in astrophysics.
- Published
- 2019
24. Newly developed Laboratory-based Size exclusion chromatography Small-angle x-ray scattering System (La-SSS)
- Author
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Rintaro Inoue, Saeko Yanaka, Tatsuo Nakagawa, Aya Okuda, Koichi Kato, Rina Yogo, Nobuhiro Sato, Masaaki Sugiyama, Maho Yagi-Utsumi, Kazuki Ito, Kazuki Omoto, Reiko Urade, and Ken Morishima
- Subjects
0301 basic medicine ,Materials science ,Size-exclusion chromatography ,lcsh:Medicine ,Characterization and analytical techniques ,Article ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Optics ,Data acquisition ,law ,lcsh:Science ,chemistry.chemical_classification ,Nanoscale biophysics ,Multidisciplinary ,Small-angle X-ray scattering ,business.industry ,Scattering ,Biomolecule ,lcsh:R ,Synchrotron ,Volumetric flow rate ,030104 developmental biology ,chemistry ,lcsh:Q ,business ,030217 neurology & neurosurgery ,Beam (structure) - Abstract
To understand a biological system, it is important to observe structures of biomolecules in the solution where the system is functionalized. Small-Angle X-ray Scattering coupled with Size Exclusion Chromatography (SEC-SAXS) is one of techniques to selectively observe the target molecules in the multi-component system. However, this technique is believed to be available only with a synchrotron-based SAXS instrument due to requirement of high beam intensity and, therefore, the limitation of the beam time was obstacle to satisfy demands from many bio-researchers. We newly developed Laboratory-based Size exclusion chromatography SAXS System (La-SSS) by utilizing a latest laboratory-based SAXS instrument and finely optimization of the balance between flow rate, cell volume, irradiation time and so on. La-SSS succeeded not only decoupling of target protein(s) from non-specific aggregates but also measurement of each concerned component in a multi-component system. In addition, an option: “stopping mode”, which is designed for improving statistics of SAXS profile, realized a high S/N data acquisition for the most interesting protein in a multi-component system. Furthermore, by utilizing a column having small bed volume, the small-scale SEC-SAXS study makes available. Through optimization of instrumental parameters and environments, La-SSS is highly applicable for experimental requirements from various biological samples. It is strongly expected that a La-SSS concept must be a normal option for laboratory-based SAXS in the near future.
- Published
- 2019
25. The Fab portion of immunoglobulin G contributes to its binding to Fcγ receptor III
- Author
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Masayoshi Onitsuka, Daisuke Higo, Rina Yogo, Takeshi Omasa, Mahito Nakanishi, Hiroki Watanabe, Yuki Yamaguchi, Shio Watanabe, Tetsuo Torisu, Mari Shimada, Saeko Yanaka, Takayuki Uchihashi, Susumu Uchiyama, Takahiro Maruno, Koichi Kato, Hirokazu Yagi, and Tadashi Satoh
- Subjects
0301 basic medicine ,Fc receptor ,lcsh:Medicine ,CHO Cells ,Immunoglobulin G ,Article ,03 medical and health sciences ,Immunoglobulin Fab Fragments ,Atomic force microscopy ,0302 clinical medicine ,Immune system ,Cricetulus ,Animals ,Humans ,lcsh:Science ,Receptor ,Multidisciplinary ,biology ,Chemistry ,Chinese hamster ovary cell ,lcsh:R ,Receptors, IgG ,biology.organism_classification ,Molecular biophysics ,Cell biology ,Immunoglobulin Fc Fragments ,030104 developmental biology ,biology.protein ,lcsh:Q ,Hydrogen–deuterium exchange ,Antibody ,Rituximab ,030217 neurology & neurosurgery - Abstract
Most cells active in the immune system express receptors for antibodies which mediate a variety of defensive mechanisms. These receptors interact with the Fc portion of the antibody and are therefore collectively called Fc receptors. Here, using high-speed atomic force microscopy, we observe interactions of human, humanized, and mouse/human-chimeric immunoglobulin G1 (IgG1) antibodies and their cognate Fc receptor, FcγRIIIa. Our results demonstrate that not only Fc but also Fab positively contributes to the interaction with the receptor. Furthermore, hydrogen/deuterium exchange mass spectrometric analysis reveals that the Fab portion of IgG1 is directly involved in its interaction with FcγRIIIa, in addition to the canonical Fc-mediated interaction. By targeting the previously unidentified receptor-interaction sites in IgG-Fab, our findings could inspire therapeutic antibody engineering.
- Published
- 2019
26. Electromagnetic Burst Generation during Annihilation of Magnetic Field in Relativistic Laser-Plasma Interaction
- Author
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J. Gu, Y., Pegoraro, F., V. Sasorov, P., Golovin, D., Yogo, A., Korn, G., and Bulanov, Sergey
- Subjects
Physics::Plasma Physics ,Physics::Space Physics - Abstract
We present the results of theoretical studies of formation and evolution of the current sheet in a colliosionless plasma during magnetic reconnection in relativistic limit. Relativistic magnetic reconnection is driven by parallel laser pulses interacting with underdense plasma target. Annihilation of laser created magnetic field of opposite polarity generates strong non-stationary electric field formed in between the region with opposite polarity magnetic field accelerating charged particles within the current sheet. This laser-plasma target configuration is discussed in regard with the laboratory modeling of charged particle acceleration and gamma flash generation in astrophysics. We present the results of 3-dimensional kinetic simulations and theoretical studies on the formation and evolution of the current sheet in a collisionless plasma during magnetic field annihilation in the ultra-relativistic limit. Annihilation of oppositively directed magnetic fields driven by two laser pulses interacting with underdense plasma target is accompanied by an electromagnetic burst generation. The induced strong non-stationary longitudinal electric field accelerates charged particles within the current sheet. Properties of the laser-plasma target configuration are discussed in the context of the laboratory modeling for charged particle acceleration and gamma flash generation in astrophysics.
- Published
- 2019
27. CETSA quantitatively verifies in vivo target engagement of novel RIPK1 inhibitors in various biospecimens
- Author
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Tsuyoshi Ishii, Masako Kuno, Masato Yoshikawa, Manabu Mochizuki, Tomohiro Kawamoto, Takuro Okai, Sachio Shibata, Takatoshi Yogo, Masanori Nakakariya, Misa Iwatani-Yoshihara, and Satoko Unno
- Subjects
0301 basic medicine ,Drug ,Thermal shift assay ,media_common.quotation_subject ,lcsh:Medicine ,Apoptosis ,Computational biology ,Peripheral blood mononuclear cell ,Article ,Automation ,Necrosis ,03 medical and health sciences ,RIPK1 ,0302 clinical medicine ,In vivo ,Animals ,Humans ,lcsh:Science ,Protein Kinase Inhibitors ,media_common ,Multidisciplinary ,Chemistry ,lcsh:R ,Temperature ,Target engagement ,Brain ,Reproducibility of Results ,Mice, Inbred C57BL ,030104 developmental biology ,Drug development ,Receptor-Interacting Protein Serine-Threonine Kinases ,030220 oncology & carcinogenesis ,Leukocytes, Mononuclear ,Biological Assay ,lcsh:Q ,HT29 Cells ,Spleen ,Intracellular - Abstract
The proof of target engagement (TE) is a key element for evaluating potential investment in drug development. The cellular thermal shift assay (CETSA) is expected to facilitate direct measurement of intracellular TE at all stages of drug development. However, there have been no reports of applying this technology to comprehensive animal and clinical studies. This report demonstrates that CETSA can not only quantitatively evaluate the drug-TE in mouse peripheral blood, but also confirm TE in animal tissues exemplified by using the receptor interacting protein 1 kinase (RIPK1) lead compound we have developed. Our established semi-automated system allows evaluation of the structure-activity relationship using native RIPK1 in culture cell lines, and also enables estimation of drug occupancy ratio in mouse peripheral blood mononuclear cells. Moreover, optimized tissue homogenisation enables monitoring of the in vivo drug-TE in spleen and brain. Our results indicate that CETSA methodology will provide an efficient tool for preclinical and clinical drug development.
- Published
- 2017
28. Boosting laser-ion acceleration with multi-picosecond pulses
- Author
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N. Iwata, Takayoshi Norimatsu, H. Shiraga, Kazuki Matsuo, N. Kamitsukasa, N. Miyanaga, Kohei Yamanoi, Akito Sagisaka, Yasunobu Arikawa, Akifumi Yogo, Shigeki Tokita, Sergei V. Bulanov, Sadaoki Kojima, Kunioki Mima, Shinsuke Fujioka, Mitsuo Nakai, Hitoshi Sakagami, Tomoyuki Johzaki, J. Kawanaka, Yasuhiko Sentoku, Hiroshi Azechi, Alessio Morace, Kiminori Kondo, Hideo Nagatomo, Hiroaki Nishimura, S. Tosaki, and Masakatsu Murakami
- Subjects
Multidisciplinary ,Materials science ,Proton ,Nuclear Theory ,Energy conversion efficiency ,Pulse duration ,Laser ,01 natural sciences ,Article ,Ponderomotive energy ,010305 fluids & plasmas ,Ion ,law.invention ,Physics::Plasma Physics ,law ,Picosecond ,0103 physical sciences ,Physics::Accelerator Physics ,Electron temperature ,Atomic physics ,Nuclear Experiment ,010306 general physics - Abstract
Using one of the world most powerful laser facility, we demonstrate for the first time that high-contrast multi-picosecond pulses are advantageous for proton acceleration. By extending the pulse duration from 1.5 to 6 ps with fixed laser intensity of 1018 W cm−2, the maximum proton energy is improved more than twice (from 13 to 33 MeV). At the same time, laser-energy conversion efficiency into the MeV protons is enhanced with an order of magnitude, achieving 5% for protons above 6 MeV with the 6 ps pulse duration. The proton energies observed are discussed using a plasma expansion model newly developed that takes the electron temperature evolution beyond the ponderomotive energy in the over picoseconds interaction into account. The present results are quite encouraging for realizing ion-driven fast ignition and novel ion beamlines.
- Published
- 2017
29. A critical role of solute carrier 22a14 in sperm motility and male fertility in mice
- Author
-
Kiyotaka Toshimori, Yu Okitsu, Keiichiro Yogo, Wataru Fujii, Yuusuke Ikami, Shin-ya Maruyama, Chizuru Ito, and Momoe Ito
- Subjects
Male ,0301 basic medicine ,Organic anion transporter 1 ,Organic Anion Transporters ,Motility ,Article ,Male infertility ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Testis ,medicine ,Animals ,Infertility, Male ,Sperm motility ,Epididymis ,Mice, Knockout ,Multidisciplinary ,Base Sequence ,biology ,Oocyte ,medicine.disease ,Spermatozoa ,Sperm ,Solute carrier family ,Cell biology ,Germ Cells ,030104 developmental biology ,medicine.anatomical_structure ,Flagella ,Mutagenesis ,Fertilization ,Immunology ,Sperm Motility ,biology.protein ,Female ,CRISPR-Cas Systems ,030217 neurology & neurosurgery - Abstract
We previously identified solute carrier 22a14 (Slc22a14) as a spermatogenesis-associated transmembrane protein in mice. Although Slc22a14 is a member of the organic anion/cation transporter family, its expression profile and physiological role have not been elucidated. Here, we show that Slc22a14 is crucial for sperm motility and male fertility in mice. Slc22a14 is expressed specifically in male germ cells, and mice lacking the Slc22a14 gene show severe male infertility. Although the overall differentiation of sperm was normal, Slc22a14−/− cauda epididymal spermatozoa showed reduced motility with abnormal flagellar bending. Further, the ability to migrate into the female reproductive tract and fertilise the oocyte were also impaired in Slc22a14−/− spermatozoa. The abnormal flagellar bending was thought to be partly caused by osmotic cell swelling since osmotic challenge or membrane permeabilisation treatment alleviated the tail abnormality. In addition, we found structural abnormalities in Slc22a14−/− sperm cells: the annulus, a ring-like structure at the mid-piece–principal piece junction, was disorganised, and expression and localisation of septin 4, an annulus component protein that is essential for the annulus formation, was also impaired. Taken together, our results demonstrated that Slc22a14 plays a pivotal role in normal flagellar structure, motility and fertility in mouse spermatozoa.
- Published
- 2016
30. Boosting laser-ion acceleration with multi-picosecond pulses.
- Author
-
Yogo, A., Mima, K., Iwata, N., Tosaki, S., Morace, A., Arikawa, Y., Fujioka, S., Johzaki, T., Sentoku, Y., Nishimura, H., Sagisaka, A., Matsuo, K., Kamitsukasa, N., Kojima, S., Nagatomo, H., Nakai, M., Shiraga, H., Murakami, M., Tokita, S., and Kawanaka, J.
- Abstract
Using one of the world most powerful laser facility, we demonstrate for the first time that high-contrast multi-picosecond pulses are advantageous for proton acceleration. By extending the pulse duration from 1.5 to 6 ps with fixed laser intensity of 1018 W cm−2, the maximum proton energy is improved more than twice (from 13 to 33 MeV). At the same time, laser-energy conversion efficiency into the MeV protons is enhanced with an order of magnitude, achieving 5% for protons above 6 MeV with the 6 ps pulse duration. The proton energies observed are discussed using a plasma expansion model newly developed that takes the electron temperature evolution beyond the ponderomotive energy in the over picoseconds interaction into account. The present results are quite encouraging for realizing ion-driven fast ignition and novel ion beamlines. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
31. A critical role of solute carrier 22a14 in sperm motility and male fertility in mice.
- Author
-
Maruyama, Shin-ya, Ito, Momoe, Ikami, Yuusuke, Okitsu, Yu, Ito, Chizuru, Toshimori, Kiyotaka, Fujii, Wataru, and Yogo, Keiichiro
- Abstract
We previously identified solute carrier 22a14 (Slc22a14) as a spermatogenesis-associated transmembrane protein in mice. Although Slc22a14 is a member of the organic anion/cation transporter family, its expression profile and physiological role have not been elucidated. Here, we show that Slc22a14 is crucial for sperm motility and male fertility in mice. Slc22a14 is expressed specifically in male germ cells, and mice lacking the Slc22a14 gene show severe male infertility. Although the overall differentiation of sperm was normal, Slc22a14
−/− cauda epididymal spermatozoa showed reduced motility with abnormal flagellar bending. Further, the ability to migrate into the female reproductive tract and fertilise the oocyte were also impaired in Slc22a14−/− spermatozoa. The abnormal flagellar bending was thought to be partly caused by osmotic cell swelling since osmotic challenge or membrane permeabilisation treatment alleviated the tail abnormality. In addition, we found structural abnormalities in Slc22a14−/− sperm cells: the annulus, a ring-like structure at the mid-piece-principal piece junction, was disorganised, and expression and localisation of septin 4, an annulus component protein that is essential for the annulus formation, was also impaired. Taken together, our results demonstrated that Slc22a14 plays a pivotal role in normal flagellar structure, motility and fertility in mouse spermatozoa. [ABSTRACT FROM AUTHOR]- Published
- 2016
- Full Text
- View/download PDF
32. Control of unsteady laser-produced plasma-flow with a multiple-coil magnetic nozzle.
- Author
-
Morita T, Edamoto M, Miura S, Sunahara A, Saito N, Itadani Y, Kojima T, Mori Y, Johzaki T, Kajimura Y, Fujioka S, Yogo A, Nishimura H, Nakashima H, and Yamamoto N
- Abstract
We report an experimental demonstration of controlling plasma flow direction with a magnetic nozzle consisting of multiple coils. Four coils are controlled separately to form an asymmetric magnetic field to change the direction of laser-produced plasma flow. The ablation plasma deforms the topology of the external magnetic field, forming a magnetic cavity inside and compressing the field outside. The compressed magnetic field pushes the plasma via the Lorentz force on a diamagnetic current: j × B in a certain direction, depending on the magnetic field configuration. Plasma and magnetic field structure formations depending on the initial magnetic field were simultaneously measured with a self-emission gated optical imager and B-dot probe, respectively, and the probe measurement clearly shows the difference of plasma expansion direction between symmetric and asymmetric initial magnetic fields. The combination of two-dimensional radiation hydrodynamic and three-dimensional hybrid simulations shows the control of the deflection angle with different number of coils, forming a plasma structure similar to that observed in the experiment.
- Published
- 2017
- Full Text
- View/download PDF
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