33 results on '"Yu, An-Lu"'
Search Results
2. Macrophage migration inhibitory factor levels are associated with disease activity and possible complications in membranous nephropathy
- Author
-
Ding, Na, Li, Peng-Lei, Wu, Kai-Li, Lv, Tie-Gang, Yu, Wen-Lu, and Hao, Jian
- Published
- 2022
- Full Text
- View/download PDF
3. Synthesis, characterization and thermal behavior of plasticized poly (vinyl chloride) doped with folic acid-modified titanium dioxide
- Author
-
Yi-heng Lu, Zong-lin Chen, and Yu-wei Lu
- Subjects
Medicine ,Science - Abstract
Abstract To inhibit the agglomeration of nanotitanium dioxide, a poly (vinyl chloride) (PVC) composite film doped with folic acid-modified titanium dioxide was synthesized and characterized using X-ray powder diffraction, X-ray photoelectron spectroscopy and thermogravimetric analysis coupled with Fourier transform infrared spectroscopy. The average grain size of the folic acid-modified titanium dioxide was found to decrease by 1.3 nm, indicating that the cohesiveness of the nanoparticles is decreased. The lowest temperature for 1.0% thermal decomposition of PVC was determined to be 230.0 °C. The decomposition rate at the peak temperature is found to be 39.6% lower than that of a control sample. The stability of the PVC is improved due to a lower number of surface chlorine atoms as well intermolecular attraction. A mechanism for folic acid modification of titanium dioxide-doped PVC is proposed. After doping, the ester groups in the plasticizer show a significant decrease in the vibration peak intensities observed at 1264 cm−1, 1736 cm−1 and 1106 cm−1. The doped PVC film suppresses the release of CO2, and the strongest vibration peak at 1264 cm−1 is found to be 17.2% lower than that for the blank sample, indicating that doping is beneficial for plasticizer recovery.
- Published
- 2022
- Full Text
- View/download PDF
4. DCE-MRI of esophageal carcinoma using star-VIBE compared with conventional 3D-VIBE
- Author
-
He-Ping Deng, Xue-Ming Li, Liu Yang, Yi Wang, Shao-Yu Wang, Peng Zhou, Yu-Jie Lu, Jin Ren, and Min Wang
- Subjects
Medicine ,Science - Abstract
Abstract To investigate the value of the star-VIBE sequence in dynamic contrast-enhanced magnetic resonance imaging of esophageal carcinoma under free breathing conditions. From February 2019 to June 2020, 60 patients with esophageal carcinoma were prospectively enrolled to undergo dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) with the K-space golden-angle radial stack-of-star acquisition scheme (star-VIBE) sequence (Group A) or conventional 3D volumetric-interpolated breath-hold examination (3D-VIBE) sequence (Group B), completely randomized grouping. The image quality of DCE-MRI was subjectively evaluated at five levels and objectively evaluated according to the image signal-to-noise ratio (SNR) and contrast-noise ratio (CNR). The DCE-MRI parameters of volume transfer constant (Ktrans), rate constant (Kep) and vascular extracellular volume fraction (Ve) were calculated using the standard Tofts double-compartment model in the post-perfusion treatment software TISSUE 4D (Siemens). Each group included 30 randomly selected cases. There was a significant difference in subjective classification between the groups (35.90 vs 25.10, p = 0.009). The study showed that both the SNR and CNR of group A were significantly higher than those of group B (p = 0.004 and 0.05). The star-VIBE sequence can be applied in DCE-MRI examination of esophageal carcinoma, which can provide higher image quality than the conventional 3D-VIBE sequence in the free breathing state.
- Published
- 2021
- Full Text
- View/download PDF
5. Outcomes and longitudinal trend of traumatic cataract wound dehiscence in patients with blunt ocular injury
- Author
-
Chiun-Ho Hou, Yu-Chin Lu, Christy Pu, Yin-Hsi Chang, Ken-Kuo Lin, Jiahn-Shing Lee, and Kuan-Jen Chen
- Subjects
Medicine ,Science - Abstract
Abstract Longitudinal trends on traumatic cataract wound dehiscence are scant. In this study, we present the characteristics of traumatic cataract wound dehiscence using 15 years of longitudinal trend in one of the largest medical centers in Taiwan for a period when cataract surgeries were gradually shifting from extracapsular cataract extraction (ECCE) to phacoemulsification. All patients with a prior cataract surgery who suffered from blunt open globe trauma between 2001 and 2015 at a tertiary referral center in Taiwan were included. The number of cases per year; type of prior cataract surgery; visual acuity (VA); mechanism and place of injury were analyzed. The risk factors associated with final VA were investigated in patients followed up for ≥ 1 month. Seventy-six eyes of 75 patients were included and all of them were traumatic cataract wound dehiscence with a prior ECCE (65 eyes) or phacoemulsification. The most common mechanism and place of injury was fall and at home in both cataract surgical types. The mean log of the minimal angle resolution (logMAR) of final VA was 2.15 ± 0.88 (ECCE) and 1.61 ± 0.83 (phacoemulsification) (P = .026). The most significant risk factors associated with worse final VA were retinal detachment at the initial visit and low ocular trauma score (both P
- Published
- 2021
- Full Text
- View/download PDF
6. Probing penile hemodynamics by using photoplethysmography as objective indicators for male erection quality and sexual function
- Author
-
Yuan-Hung Pong, Yi-Kai Chang, Ching-En Hsu, Po-Cheng Chen, Yu-Chuan Lu, Vincent F. S. Tsai, Hong-Chiang Chang, Men-Tzung Lo, and Chen Lin
- Subjects
Medicine ,Science - Abstract
Abstract Erectile dysfunction (ED) is mostly due to the lack of blood flow into the penis. In the past 20 years, near-infrared spectroscopy (NIRS) was used in measuring the concentrations and temporal dynamics of different hemoglobin types. However, the dynamics of the light absorption (photoplethysmography; PPG) have not been applied to survey penile hemodynamics and erection quality. This paper compared the use of photoplethysmography (PPG) to study vascular ED with standard penile Doppler ultrasonography. Men diagnosed with vascular ED for at least 6 months and nominated for penile ultrasonography were included. PPG signals were collected during the ultrasound examination. All beat-to-beat PPG waveforms were aligned with the peak and averaged to one representative template waveform for feature analysis, including amplitude differences (APD) index, reflection time index (RTI), augmentation index (AI), and perfusion index (PI). An inverse correlation was found between end-erection amplitude and both erection hardness score (EHS) and resistive index (RI). APD index and EHS as well as the international index of erectile function-5 (IIEF) and RI were positively correlated. RTI and AI were inversely correlated to IIEF and RI. PI was positively correlated to RI. PPG may therefore be useful as a noninvasive, convenient, technique for sexual function evaluation.
- Published
- 2021
- Full Text
- View/download PDF
7. Ultra-broadband, lithography-free, omnidirectional, and polarization-insensitive perfect absorber
- Author
-
Tse-An Chen, Meng-Ju Yub, Yu-Jung Lu, and Ta-Jen Yen
- Subjects
Medicine ,Science - Abstract
Abstract Perfect absorbers (PAs) at near infrared allow various applications such as biosensors, nonlinear optics, color filters, thermal emitters and so on. These PAs, enabled by plasmonic resonance, are typically powerful and compact, but confront inherent challenges of narrow bandwidth, polarization dependence, and limited incident angles as well as requires using expensive lithographic process, which limit their practical applications and mass production. In this work, we demonstrate a non-resonant PA that is comprised of six continuous layers of magnesium fluoride (MgF2) and chromium (Cr) in turns. Our device absorbs more than 90% of light in a broad range of 900–1900 nm. In addition, such a planar design is lithography-free, certainly independent with polarization, and presents a further advantage of wide incidence up to 70°. The measured performance of our optimized PA agrees well with analytical calculations of transfer matrix method (TMM) and numerical simulations of finite element method, and can be readily implemented for practical applications.
- Published
- 2021
- Full Text
- View/download PDF
8. Influence of Surface Roughness on Strong Light-Matter Interaction of a Quantum Emitter-Metallic Nanoparticle System
- Author
-
Yu-Wei Lu, Ling-Yan Li, and Jing-Feng Liu
- Subjects
Medicine ,Science - Abstract
Abstract We investigate the quantum optical properties of strong light-matter interaction between a quantum emitter and a metallic nanoparticle beyond idealized structures with a smooth surface. Based on the local coupling strength and macroscopic Green’s function, we derived an exact quantum optics approach to obtain the field enhancement and light-emission spectrum of a quantum emitter. Numerical simulations show that the surface roughness has a greater effect on the near-field than on the far-field, and slightly increases the vacuum Rabi splitting on average. Further, we verified that the near-field enhancement is mainly determined by the surface features of hot-spot area.
- Published
- 2018
- Full Text
- View/download PDF
9. Association of S100B polymorphisms and serum S100B with risk of ischemic stroke in a Chinese population
- Author
-
Yu-Lan Lu, Rong Wang, Hua-Tuo Huang, Hai-Mei Qin, Chun-Hong Liu, Yang Xiang, Chun-Fang Wang, Hong-Cheng Luo, Jun-Li Wang, Yan Lan, and Ye-Sheng Wei
- Subjects
Medicine ,Science - Abstract
Abstract The levels of serum S100B were elevated in patients with ischemic stroke (IS), which may be a novel biomarker for diagnosing IS. The aim of this study was to investigate the association of S100B polymorphisms and serum S100B with IS risk. We genotyped the S100B polymorphisms rs9722, rs9984765, rs2839356, rs1051169 and rs2186358 in 396 IS patients and 398 controls using polymerase chain reaction-single base extension (SBE-PCR). Serum S100B levels were measured by enzyme-linked immunosorbent assay (ELISA). Rs9722 was associated with an increased risk of IS (AA vs. GG: adjusted OR = 2.172, 95% CI, 1.175–4.014, P = 0.013; dominant: adjusted OR = 1.507, 95% CI, 1.071–2.123, P = 0.019; recessive: adjusted OR = 1.846, 95% CI, 1.025–3.323, P = 0.041; additive: adjusted OR=1.371, 95% CI, 1.109-1.694, P = 0.003). The A-C-C-C-A haplotype was associated with an increased risk of IS (OR = 1.325, 95% CI, 1.035–1.696, P = 0.025). In addition, individuals carrying the rs9722 GA/AA genotypes had a higher serum S100B compared with the rs9722 GG genotype in IS patients (P = 0.018). Our results suggest that the S100B gene rs9722 polymorphism may contribute to the susceptibility of IS, probably by promoting the expression of serum S100B.
- Published
- 2018
- Full Text
- View/download PDF
10. The long non-coding RNA LOC441204 enhances cell growth in human glioma
- Author
-
Tzu-Kang Lin, Chang-Nen Chang, Cheng-Shian Tsai, Yin-Cheng Huang, Yu-Jen Lu, Wei-Jan Chen, Yang-Hsiang Lin, I.-Hsiao Chung, and Kwang-Huei Lin
- Subjects
Medicine ,Science - Abstract
Abstract Glioma is the most common and aggressive type of brain tumor. While long non-coding RNAs (lncRNAs) are clearly more abundant in human brain than protein-coding genes, the specific roles of lncRNAs and mechanisms underlying their dysregulation in glioma remain unclear. Here, we focused on lncRNAs that are differentially expressed in brain tumor and their potential biological functions. LOC441204, a novel non-coding RNA gene displaying high expression in clinical specimens of brain tumor and significant upregulation in glioma cell lines in microarray analyses, was selected for further study. Notably, knockdown of LOC441204 suppressed tumor cell proliferation in two glioma cell lines. Moreover, LOC441204-induced tumor cell growth was mediated the stabilization of β-catenin pathway. Briefly, LOC441204 bound to β-catenin preventing its degradation, resulting in downstream p21 repression and cdk4 activation to enhance glioma cell proliferation. Collectively, our findings indicate a pro-oncogenic role of LOC441204 in tumor cell growth through activation of the β-catenin/p21/cdk4 cascade to act as a potential diagnostic marker or therapeutic target in brain tumor.
- Published
- 2017
- Full Text
- View/download PDF
11. Propensity score matching analysis comparing radical prostatectomy and radiotherapy with androgen deprivation therapy in locally advanced prostate cancer
- Author
-
Yu-Cheng Lu, Chao-Yuan Huang, Chia-Hsien Cheng, Kuo-How Huang, Yu-Chuan Lu, Po-Ming Chow, Yi-Kai Chang, Yeong-Shiau Pu, Chung-Hsin Chen, Shao-Lun Lu, Keng-Hsueh Lan, Fu-Shan Jaw, Pei-Ling Chen, and Jian-Hua Hong
- Subjects
Male ,Prostatectomy ,Multidisciplinary ,Androgens ,Humans ,Prostatic Neoplasms ,Androgen Antagonists ,Neoplasm Recurrence, Local ,Prostate-Specific Antigen ,Propensity Score ,Retrospective Studies - Abstract
To compare clinical outcomes between the use of robotic-assisted laparoscopic radical prostatectomy (RP) and radiotherapy (RT) with long-term androgen deprivation therapy (ADT) in locally advanced prostate cancer (PC), 315 patients with locally advanced PC (clinical T-stage 3/4) were considered for analysis retrospectively. Propensity score-matching at a 1:1 ratio was performed. The median follow-up period was 59.2 months (IQR 39.8–87.4). There were 117 (37.1%) patients in the RP group and 198 (62.9%) patients in the RT group. RT patients were older and had higher PSA at diagnosis, higher Gleason score grade group and more advanced T-stage (all p p p = 0.470, p = 0.268 and p = 0.509, respectively). This information supported a clinical benefit in BCR control for patients undergoing RT plus long-term ADT compared to RP.
- Published
- 2021
12. Preoperative %p2PSA and Prostate Health Index Predict Pathological Outcomes in Patients with Prostate Cancer Undergoing Radical Prostatectomy
- Author
-
Kuo-How Huang, Yeong-Shiau Pu, Yu-Chuan Lu, Shih-Ting Chiu, Hong-Chiang Chang, Chao-Yuan Huang, Yuan-Ju Lee, Jian-Hua Hong, Chih-Hung Chiang, I-Ni Chiang, Shih-Ping Liu, Shih-Chun Hung, Chung-Hsin Chen, Yung-Ting Cheng, Po-Ming Chow, and Yi-Kai Chang
- Subjects
Male ,medicine.medical_specialty ,medicine.medical_treatment ,030232 urology & nephrology ,Urology ,lcsh:Medicine ,Disease ,Article ,Tumour biomarkers ,03 medical and health sciences ,Prostate cancer ,0302 clinical medicine ,Prostate ,Biomarkers, Tumor ,medicine ,Humans ,Prospective Studies ,Prospective cohort study ,lcsh:Science ,Pathological ,Aged ,Prostatectomy ,Multidisciplinary ,Receiver operating characteristic ,business.industry ,lcsh:R ,Prostatic Neoplasms ,Middle Aged ,Prostate-Specific Antigen ,Prognosis ,medicine.disease ,Treatment Outcome ,medicine.anatomical_structure ,ROC Curve ,030220 oncology & carcinogenesis ,Preoperative Period ,lcsh:Q ,Positive Surgical Margin ,business - Abstract
To evaluate the predictive accuracy of the %p2PSA and prostate health index (PHI) in predicting aggressive pathological outcomes in patients with prostate cancer (PCa) undergoing radical prostatectomy (RP), we enrolled 91 patients with organ-confined PCa who were treated with robot-assisted RP. p2PSA levels and the PHI were investigated for their ability to predict pathological results. The %p2PSA and PHI were both significantly higher in patients with ≥pT3 disease, high-risk disease, positive surgical margin, or seminal vesical invasion (SVI). In univariable analysis, p2PSA derivatives were significant predictors of the presence of ≥pT3 disease, high-risk disease, positive surgical margin, and SVI. To predict adverse pathological outcomes at a sensitivity of 90%, p2PSA derivatives had higher specificity than standard PSA derivatives. In multivariable analysis, additional increases in the area under the receiver operating characteristic curve (AUC) were observed with the %p2PSA and PHI for ≥pT3 disease, high-risk disease, and positive surgical margin (8.2% and 2.7%, 6.2% and 4.1%, and 8.6% and 5.4%, respectively). A PHI ≥61.26 enhanced the predictive accuracy of the model for SVI by increasing the AUC from 0.624 to 0.819 (p = 0.009). The preoperative %p2PSA and PHI accurately predict adverse pathological results and are useful for decision-making.
- Published
- 2020
13. Outcomes and longitudinal trend of traumatic cataract wound dehiscence in patients with blunt ocular injury
- Author
-
Yin Hsi Chang, Kuan-Jen Chen, Chiun Ho Hou, Ken Kuo Lin, Yu Chin Lu, Christy Pu, and Jiahn Shing Lee
- Subjects
Male ,medicine.medical_specialty ,Visual acuity ,genetic structures ,medicine.medical_treatment ,Science ,Visual Acuity ,Cataract Extraction ,Article ,Blunt ,Medical research ,Eye Injuries ,Surgical Wound Dehiscence ,medicine ,Humans ,In patient ,Aged ,Aged, 80 and over ,Multidisciplinary ,Accidental Injuries ,business.industry ,Wound dehiscence ,Retinal detachment ,Phacoemulsification ,Cataract surgery ,Middle Aged ,medicine.disease ,eye diseases ,Surgery ,Risk factors ,Blunt trauma ,Medicine ,Accidental Falls ,Female ,sense organs ,medicine.symptom ,business - Abstract
Longitudinal trends on traumatic cataract wound dehiscence are scant. In this study, we present the characteristics of traumatic cataract wound dehiscence using 15 years of longitudinal trend in one of the largest medical centers in Taiwan for a period when cataract surgeries were gradually shifting from extracapsular cataract extraction (ECCE) to phacoemulsification. All patients with a prior cataract surgery who suffered from blunt open globe trauma between 2001 and 2015 at a tertiary referral center in Taiwan were included. The number of cases per year; type of prior cataract surgery; visual acuity (VA); mechanism and place of injury were analyzed. The risk factors associated with final VA were investigated in patients followed up for ≥ 1 month. Seventy-six eyes of 75 patients were included and all of them were traumatic cataract wound dehiscence with a prior ECCE (65 eyes) or phacoemulsification. The most common mechanism and place of injury was fall and at home in both cataract surgical types. The mean log of the minimal angle resolution (logMAR) of final VA was 2.15 ± 0.88 (ECCE) and 1.61 ± 0.83 (phacoemulsification) (P = .026). The most significant risk factors associated with worse final VA were retinal detachment at the initial visit and low ocular trauma score (both P
- Published
- 2021
14. Probing penile hemodynamics by using photoplethysmography as objective indicators for male erection quality and sexual function
- Author
-
Yi Kai Chang, Chen Lin, Men Tzung Lo, Po Cheng Chen, Ching En Hsu, Vincent F.S. Tsai, Yu Chuan Lu, Hong-Chiang Chang, and Yuan Hung Pong
- Subjects
Male ,medicine.medical_specialty ,Urology ,Science ,Erection hardness score ,030232 urology & nephrology ,Hemodynamics ,Article ,03 medical and health sciences ,0302 clinical medicine ,Photoplethysmogram ,Internal medicine ,medicine ,Humans ,Photoplethysmography ,Aged ,Multidisciplinary ,business.industry ,Penile Erection ,Ultrasound ,Blood flow ,Middle Aged ,medicine.disease ,Urogenital diseases ,medicine.anatomical_structure ,Erectile dysfunction ,030220 oncology & carcinogenesis ,Cardiology ,Medicine ,business ,Sexual function ,Penis - Abstract
Erectile dysfunction (ED) is mostly due to the lack of blood flow into the penis. In the past 20 years, near-infrared spectroscopy (NIRS) was used in measuring the concentrations and temporal dynamics of different hemoglobin types. However, the dynamics of the light absorption (photoplethysmography; PPG) have not been applied to survey penile hemodynamics and erection quality. This paper compared the use of photoplethysmography (PPG) to study vascular ED with standard penile Doppler ultrasonography. Men diagnosed with vascular ED for at least 6 months and nominated for penile ultrasonography were included. PPG signals were collected during the ultrasound examination. All beat-to-beat PPG waveforms were aligned with the peak and averaged to one representative template waveform for feature analysis, including amplitude differences (APD) index, reflection time index (RTI), augmentation index (AI), and perfusion index (PI). An inverse correlation was found between end-erection amplitude and both erection hardness score (EHS) and resistive index (RI). APD index and EHS as well as the international index of erectile function-5 (IIEF) and RI were positively correlated. RTI and AI were inversely correlated to IIEF and RI. PI was positively correlated to RI. PPG may therefore be useful as a noninvasive, convenient, technique for sexual function evaluation.
- Published
- 2021
15. Synthesis, characterization and thermal behavior of plasticized poly (vinyl chloride) doped with folic acid-modified titanium dioxide
- Author
-
Yi-heng Lu, Zong-lin Chen, and Yu-wei Lu
- Subjects
Titanium ,Multidisciplinary ,Folic Acid ,Plasticizers ,Vinyl Chloride ,Polyvinyl Chloride - Abstract
To inhibit the agglomeration of nanotitanium dioxide, a poly (vinyl chloride) (PVC) composite film doped with folic acid-modified titanium dioxide was synthesized and characterized using X-ray powder diffraction, X-ray photoelectron spectroscopy and thermogravimetric analysis coupled with Fourier transform infrared spectroscopy. The average grain size of the folic acid-modified titanium dioxide was found to decrease by 1.3 nm, indicating that the cohesiveness of the nanoparticles is decreased. The lowest temperature for 1.0% thermal decomposition of PVC was determined to be 230.0 °C. The decomposition rate at the peak temperature is found to be 39.6% lower than that of a control sample. The stability of the PVC is improved due to a lower number of surface chlorine atoms as well intermolecular attraction. A mechanism for folic acid modification of titanium dioxide-doped PVC is proposed. After doping, the ester groups in the plasticizer show a significant decrease in the vibration peak intensities observed at 1264 cm−1, 1736 cm−1 and 1106 cm−1. The doped PVC film suppresses the release of CO2, and the strongest vibration peak at 1264 cm−1 is found to be 17.2% lower than that for the blank sample, indicating that doping is beneficial for plasticizer recovery.
- Published
- 2021
16. Genetic and phenotypic diversity of methicillin-resistant Staphylococcus aureus among Japanese inpatients in the early 1980s
- Author
-
Yu Jie Lu, Hui Zuo, Keiichi Hiramatsu, Yuki Uehara, and Takashi Sasaki
- Subjects
0301 basic medicine ,clone (Java method) ,Male ,Methicillin-Resistant Staphylococcus aureus ,Imipenem ,Virulence Factors ,Science ,030106 microbiology ,Population ,Biology ,medicine.disease_cause ,Article ,Microbiology ,03 medical and health sciences ,Asian People ,Japan ,Drug Resistance, Multiple, Bacterial ,Genotype ,medicine ,Humans ,education ,Clinical microbiology ,education.field_of_study ,Inpatients ,Multidisciplinary ,SCCmec ,Chromosome ,respiratory system ,biochemical phenomena, metabolism, and nutrition ,History, 20th Century ,Staphylococcal Infections ,bacterial infections and mycoses ,Methicillin-resistant Staphylococcus aureus ,030104 developmental biology ,Staphylococcus aureus ,Medicine ,Infectious diseases ,Female ,medicine.drug - Abstract
To trace the linkage between Japanese healthcare-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) strains in the early 1980s and the 2000s onward, we performed molecular characterizations using mainly whole-genome sequencing. Among the 194 S. aureus strains isolated, 20 mecA-positive MRSA (10.3%), 8 mecA-negative MRSA (4.1%) and 3 mecA-positive methicillin-susceptible S. aureus (MSSA) (1.5%) strains were identified. The most frequent sequence type (ST) was ST30 (n = 11), followed by ST5 (n = 8), ST81 (n = 4), and ST247 (n = 3). Rates of staphylococcal cassette chromosome mec (SCCmec) types I, II, and IV composed 65.2%, 13.0%, and 17.4% of isolates, respectively. Notably, 73.3% of SCCmec type I strains were susceptible to imipenem unlike SCCmec type II strains (0%). ST30-SCCmec I (n = 7) and ST5-SCCmec I (n = 5) predominated, whereas only two strains exhibited imipenem-resistance and were tst-positive ST5-SCCmec II, which is the current Japanese HA-MRSA genotype. All ST30 strains shared the common ancestor strain 55/2053, which caused the global pandemic of Panton-Valentine leukocidin-positive MSSA in Europe and the United States in the 1950s. Conspicuously more heterogeneous, the population of HA-MRSA clones observed in the 1980s, including the ST30-SCCmec I clone, has shifted to the current homogeneous population of imipenem-resistant ST5-SCCmec II clones, probably due to the introduction of new antimicrobials.
- Published
- 2021
17. The impact of primary location and age at orchiopexy on testicular atrophy for congenital undescended testis
- Author
-
Yu-Chuan Lu, Chi-Shin Tseng, Yeong-Shiau Pu, Ming-Chieh Kuo, Chao-Yuan Huang, Chung-Hung Hong, Chung-Hsin Chen, Hong-Chiang Chang, Kuo-How Huang, and I-Ni Chiang
- Subjects
Paediatric urology ,Adult ,Male ,medicine.medical_specialty ,Adolescent ,medicine.medical_treatment ,Urology ,lcsh:Medicine ,Article ,Testis ,Cryptorchidism ,medicine ,Endocrine system ,Humans ,Orchiopexy ,Child ,lcsh:Science ,Retrospective Studies ,Multidisciplinary ,Testicular atrophy ,business.industry ,Incidence (epidemiology) ,Hazard ratio ,lcsh:R ,Retrospective cohort study ,medicine.disease ,Confidence interval ,Premature birth ,lcsh:Q ,business ,Follow-Up Studies - Abstract
In this study, we investigated post-orchiopexy testicular growth of undescended testes (UDTs) at different primary locations and determined the risk factors for testicular atrophy (TA). We conducted a retrospective chart review of boys who had undergone orchiopexy for UDTs during January 2001–December 2013. Patient profile, age at operation, primary UDT location, and testicular volume were noted. TA was defined as ≥50% loss of volume after orchiopexy. The primary endpoints were testicular growth and TA after orchiopexy. The secondary endpoint was risk factors for TA. In total, 182 boys had undergone regular ultrasonography; the median follow-up period was 34 months. Among 230 UDTs, 18 (7.8%) atrophic testicles were identified within a median interval of 13 months after orchiopexy. TA rates were 3.3% (1/30), 6.9% (12/173), and 18.5% (5/27) in primary suprascrotal, canalicular, and above-inguinal UDTs, respectively. The survival probability of UDT was 91%, 92% and 100% when orchiopexy was performed in age ≤1 year, 1 2 years, respectively. Multivariate analysis revealed that inguinal and above-inguinal UDTs (hazard ratio [HR] 11.76, 95% confidence interval [CI] 1.55–89.33, p = 0.017) and genetic or endocrine disorders (HR 3.19, 95% CI 1.19–8.56, p = 0.021) were the risk factors for TA, but not age at operation, premature birth, and laterality. Thus, TA incidence was higher when patients had high primary testicular locations. Early orchiopexy before two years of age may be associated with higher TA risk, while most testicles have promising growth after orchiopexy.
- Published
- 2019
18. Associations between Peripheral Thromboembolic Vascular Disease and Androgen Deprivation Therapy in Asian Prostate Cancer Patients
- Author
-
Chao-Hsiang Chang, Chi Jung Chung, Shiu-Dong Chung, Yu-Chuan Lu, Chao-Yuan Huang, Fu-Shan Jaw, Chih-Hsin Muo, Huei-Ming Yeh, Teng-Kai Yang, and Jian-Hua Hong
- Subjects
Male ,Deep vein ,lcsh:Medicine ,Disease ,Comorbidity ,Kaplan-Meier Estimate ,030204 cardiovascular system & hematology ,Androgen deprivation therapy ,Prostate cancer ,0302 clinical medicine ,Thrombophilia ,lcsh:Science ,Chemical castration ,Cancer ,Multidisciplinary ,Incidence ,Prostate ,Middle Aged ,Thrombosis ,Combined Modality Therapy ,Pulmonary embolism ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Androgens ,Risk ,medicine.medical_specialty ,Antineoplastic Agents, Hormonal ,Taiwan ,Adenocarcinoma ,Article ,03 medical and health sciences ,Peripheral Arterial Disease ,Asian People ,Internal medicine ,Thromboembolism ,medicine ,Humans ,Aged ,Proportional Hazards Models ,Retrospective Studies ,Prostatectomy ,Vascular disease ,business.industry ,lcsh:R ,Prostatic Neoplasms ,Androgen Antagonists ,medicine.disease ,lcsh:Q ,Radiotherapy, Adjuvant ,business ,Pulmonary Embolism ,Orchiectomy - Abstract
This study aimed to investigate the risks of thromboembolic vascular disease following androgen deprivation therapy (ADT) administered to prostate cancer (PCa) patients. A total of 24,464 men with newly diagnosed PCa during 2000–2008 were recruited through a longitudinal health insurance database in Taiwan. All PCa patients were stratified into two: ADT and non-ADT groups. Patients with ADT treatment were grouped into three: surgical castration, chemical castration, and anti-androgen alone. The risks of pulmonary embolism (PE), peripheral arterial occlusion disease (PAOD), and deep vein thrombosis (DVT) were assessed in multiple Cox proportional-hazards regression with time-dependent covariates. During the 12-year follow-up period, incidence rates per 1000 person-years in ADT and non-ADT groups were 2.87 and 1.62 for DVT, 1.00 and 0.52 for PE, and 1.03 and 0.70 for PAOD, respectively. The DVT and PE risks were significantly increased in patients receiving combined androgen blockade (CAB) compared with the counterpart ADT non-recipients. After adjusting for potential risk factors, PCa patients receiving CAB had the highest PE risk (HR = 3.11), followed by DVT risk (HR = 2.53). The DVT risk remained elevated throughout the entire duration of chemical castration. However, high PE risk was observed in patients with ≤720-day treatment duration. No association was found between ADT and PAOD risks. Overall, the risks of PE and DVT were considerably heightened in Asian men subjected to CAB for PCa, whereas PAOD risk was unrelated to such treatments.
- Published
- 2019
19. Deciphering the Odorant Binding, Activation, and Discrimination Mechanism of Dhelobp21 from Dastarus Helophoroides
- Author
-
Yu-Lin Lu, Guang-Qiang Yu, Dong-Zhen Li, Ya-Qi Wang, De-Xin Kong, and Man-Qun Wang
- Subjects
0301 basic medicine ,Olfactory system ,Odorant binding ,lcsh:Medicine ,Plasma protein binding ,Molecular Dynamics Simulation ,Receptors, Odorant ,Protein Structure, Secondary ,Article ,03 medical and health sciences ,Molecular dynamics ,Protein structure ,Sequence Homology, Nucleic Acid ,Animals ,Amino Acid Sequence ,lcsh:Science ,Peptide sequence ,Multidisciplinary ,Chemistry ,lcsh:R ,Coleoptera ,Smell ,030104 developmental biology ,Odorants ,Helix ,Nucleic acid ,Biophysics ,Insect Proteins ,lcsh:Q ,Protein Binding - Abstract
Odorant-binding proteins (OBPs) play a pivotal role in transporting odorants through the sensillar lymph of insect chemosensory sensilla and increasing the sensitivity of the olfactory system. To address the ligand binding, activation, and release mechanisms of OBPs, we performed a set of conventional molecular dynamics simulations for binding of the odorant-binding protein DhelOBP21 from Dastarcus helophoroides with 18 ligands (1-NPN and 17 volatiles), as well as four constant-pH molecular dynamics simulations. We found that the open pocket DhelOBP21 at pH 5.0 could bind volatiles and form a closed pocket complex via transformation of its N-terminus into regular Helix at pH 7.0 and vice versa. Moreover, the discrimination of volatiles (selectivity and promiscuity) was determined by the characteristics of both the volatiles and the ‘essential’ and ‘selective’ amino acid residues in OBP binding pockets, rather than the binding affinity of the volatiles. This study put forward a new hypothesis that during the binding of volatiles there are two transitions for the DhelOBP21 amino-terminus: pH- and odorant binding-dependent random-coil-to-helix. Another important finding is providing a framework for the exploration of the complete coil-to-helix transition process and theoretically analyzing its underlying causes at molecular level.
- Published
- 2018
20. Influence of Surface Roughness on Strong Light-Matter Interaction of a Quantum Emitter-Metallic Nanoparticle System
- Author
-
Jing-Feng Liu, Ling-Yan Li, and Yu-Wei Lu
- Subjects
Surface (mathematics) ,Quantum optics ,Multidisciplinary ,Materials science ,Field (physics) ,Condensed matter physics ,Science ,Nanoparticle ,02 engineering and technology ,021001 nanoscience & nanotechnology ,01 natural sciences ,Article ,Metal ,visual_art ,0103 physical sciences ,visual_art.visual_art_medium ,Surface roughness ,Medicine ,010306 general physics ,0210 nano-technology ,Quantum ,Quantum emitter - Abstract
We investigate the quantum optical properties of strong light-matter interaction between a quantum emitter and a metallic nanoparticle beyond idealized structures with a smooth surface. Based on the local coupling strength and macroscopic Green’s function, we derived an exact quantum optics approach to obtain the field enhancement and light-emission spectrum of a quantum emitter. Numerical simulations show that the surface roughness has a greater effect on the near-field than on the far-field, and slightly increases the vacuum Rabi splitting on average. Further, we verified that the near-field enhancement is mainly determined by the surface features of hot-spot area.
- Published
- 2018
21. Association of S100B polymorphisms and serum S100B with risk of ischemic stroke in a Chinese population
- Author
-
Jun-Li Wang, Chun-Fang Wang, Yan Lan, Hong-Cheng Luo, Yang Xiang, Ye-Sheng Wei, Hua-Tuo Huang, Yu-Lan Lu, Hai-Mei Qin, Chun-Hong Liu, and Rong Wang
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,Science ,S100 Calcium Binding Protein beta Subunit ,Polymorphism, Single Nucleotide ,Gastroenterology ,Article ,Brain Ischemia ,03 medical and health sciences ,0302 clinical medicine ,Asian People ,Gene Frequency ,Risk Factors ,Internal medicine ,Genotype ,Humans ,Medicine ,Genetic Predisposition to Disease ,In patient ,Nerve Growth Factors ,Chinese population ,Multidisciplinary ,business.industry ,Haplotype ,Middle Aged ,Stroke ,030104 developmental biology ,Increased risk ,Haplotypes ,Ischemic stroke ,Female ,business ,030217 neurology & neurosurgery - Abstract
The levels of serum S100B were elevated in patients with ischemic stroke (IS), which may be a novel biomarker for diagnosing IS. The aim of this study was to investigate the association of S100B polymorphisms and serum S100B with IS risk. We genotyped the S100B polymorphisms rs9722, rs9984765, rs2839356, rs1051169 and rs2186358 in 396 IS patients and 398 controls using polymerase chain reaction-single base extension (SBE-PCR). Serum S100B levels were measured by enzyme-linked immunosorbent assay (ELISA). Rs9722 was associated with an increased risk of IS (AA vs. GG: adjusted OR = 2.172, 95% CI, 1.175–4.014, P = 0.013; dominant: adjusted OR = 1.507, 95% CI, 1.071–2.123, P = 0.019; recessive: adjusted OR = 1.846, 95% CI, 1.025–3.323, P = 0.041; additive: adjusted OR=1.371, 95% CI, 1.109-1.694, P = 0.003). The A-C-C-C-A haplotype was associated with an increased risk of IS (OR = 1.325, 95% CI, 1.035–1.696, P = 0.025). In addition, individuals carrying the rs9722 GA/AA genotypes had a higher serum S100B compared with the rs9722 GG genotype in IS patients (P = 0.018). Our results suggest that the S100B gene rs9722 polymorphism may contribute to the susceptibility of IS, probably by promoting the expression of serum S100B.
- Published
- 2018
22. Zebrafish VCAP1X2 regulates cardiac contractility and proliferation of cardiomyocytes and epicardial cells
- Author
-
Fang Chi Hsieh, Sheng-Ping L. Hwang, Chao-Min Cheng, Chien-Chang Chen, Ian Liau, Chung-Der Hsiao, and Yu Fen Lu
- Subjects
0301 basic medicine ,Sarcomeres ,Myofilament ,Heart Ventricles ,lcsh:Medicine ,Vascular Cell Adhesion Molecule-1 ,Vascular Cell Adhesion Protein 1 ,Sarcomere ,Sodium-Calcium Exchanger ,Article ,Contractility ,03 medical and health sciences ,Myofibrils ,Animals ,Protein Isoforms ,Myocytes, Cardiac ,Phosphorylation ,lcsh:Science ,Extracellular Signal-Regulated MAP Kinases ,PI3K/AKT/mTOR pathway ,Zebrafish ,Cell Proliferation ,Multidisciplinary ,Myosin Heavy Chains ,Chemistry ,Activator (genetics) ,lcsh:R ,MYPN ,Zebrafish Proteins ,Myocardial Contraction ,Cell biology ,030104 developmental biology ,Mutagenesis ,lcsh:Q ,Calcium ,MYH6 ,Proto-Oncogene Proteins c-akt - Abstract
Sarcomeric signaling complexes are important to sustain proper sarcomere structure and function, however, the mechanisms underlying these processes are not fully elucidated. In a gene trap experiment, we found that vascular cell adhesion protein 1 isoform X2 (VCAP1X2) mutant embryos displayed a dilated cardiomyopathy phenotype, including reduced cardiac contractility, enlarged ventricular chamber and thinned ventricular compact layer. Cardiomyocyte and epicardial cell proliferation was decreased in the mutant heart ventricle, as was the expression of pAKT and pERK. Contractile dysfunction in the mutant was caused by sarcomeric disorganization, including sparse myofilament, blurred Z-disc, and decreased gene expression for sarcomere modulators (smyd1b, mypn and fhl2a), sarcomeric proteins (myh6, myh7, vmhcl and tnnt2a) and calcium regulators (ryr2b and slc8a1a). Treatment of PI3K activator restored Z-disc alignment while injection of smyd1b mRNA restored Z-disc alignment, contractile function and cardiomyocyte proliferation in ventricles of VCAP1X2 mutant embryos. Furthermore, injection of VCAP1X2 variant mRNA rescued all phenotypes, so long as two cytosolic tyrosines were left intact. Our results reveal two tyrosine residues located in the VCAP1X2 cytoplasmic domain are essential to regulate cardiac contractility and the proliferation of ventricular cardiomyocytes and epicardial cells through modulating pAKT and pERK expression levels.
- Published
- 2018
23. Advantage of early orchiopexy for undescended testis: Analysis of testicular growth percentage ratio in patients with unilateral undescended testicle
- Author
-
Hong-Chiang Chang, Chung-Hung Hong, Chi-Shin Tseng, Yu-Chuan Lu, Yeong-Shiau Pu, Kuo-How Huang, I-Ni Chiang, and Jian-Hua Hong
- Subjects
Male ,medicine.medical_specialty ,medicine.medical_treatment ,030232 urology & nephrology ,lcsh:Medicine ,Article ,Time-to-Treatment ,03 medical and health sciences ,0302 clinical medicine ,Age groups ,Cryptorchidism ,Testis ,Medicine ,Humans ,In patient ,Orchiopexy ,030212 general & internal medicine ,lcsh:Science ,Retrospective Studies ,Ultrasonography ,Multidisciplinary ,business.industry ,lcsh:R ,Age Factors ,Infant ,Retrospective cohort study ,Testicular growth ,Organ Size ,medicine.disease ,Surgery ,Undescended testicle ,Treatment Outcome ,Child, Preschool ,Referral center ,lcsh:Q ,business ,Follow-Up Studies - Abstract
This study reports the experience of our tertiary referral center and proposes a new indicator, the growth percentage ratio (GPR), for determining the optimal timing of surgical intervention. A retrospective review of boys who underwent orchiopexy for undescended testis from 2001 to 2013 was conducted. We analyzed testicular volumes in different age groups using the UDT to normally descended testis ratio and testicular GPR. A total of 134 boys with unilateral undescended testicle underwent regular ultrasonography follow-up examinations for more than a mean of 3.9 years. Forty-five (33.4%) of them underwent orchiopexy before the age of one year. Orchiopexy at this age resulted in a GPR (2.02 ± 0.40) that was significantly higher than the GPRs in the second (1 2 years, 1.24 ± 0.14 p = 0.008) age groups. The undescended testicle grew faster when orchiopexy was performed before one year of age. Orchiopexy performed within one year from birth significantly accelerates the growth of the UDT, as determined using the GPR, compared to other age groups. The present clinical evidence indicates that orchiopexy should be performed before one year of age.
- Published
- 2017
24. Cross-Talk Immunity of PEDOT:PSS Pressure Sensing Arrays with Gold Nanoparticle Incorporation
- Author
-
Jyh-Wei Lee, Jer-Chyi Wang, Yi Fu, Yu-Jen Lu, Rajat Subhra Karmakar, Tzu-Kang Lin, Kuo-Chen Wei, Ming-Chung Wu, and Shun-Hsiang Chan
- Subjects
Materials science ,Scanning electron microscope ,Polymers ,Hydrostatic pressure ,lcsh:Medicine ,Nanoparticle ,Metal Nanoparticles ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Article ,PEDOT:PSS ,Microscopy, Electron, Transmission ,Microscopy ,Hydrostatic Pressure ,Dermatoglyphics ,lcsh:Science ,Multidisciplinary ,business.industry ,lcsh:R ,Electric Conductivity ,Spectrometry, X-Ray Emission ,021001 nanoscience & nanotechnology ,Bridged Bicyclo Compounds, Heterocyclic ,Piezoresistive effect ,0104 chemical sciences ,Electrode ,Microscopy, Electron, Scanning ,Optoelectronics ,Polystyrenes ,lcsh:Q ,Nanoindenter ,Gold ,0210 nano-technology ,business - Abstract
In this study, the cross-talk effects and the basic piezoresistive characteristics of gold nanoparticle (Au-NP) incorporated poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) pressure sensing 2 × 2 arrays are investigated using a cross-point electrode (CPE) structure. Transmission electron microscopy (TEM), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDS) mappings were carried out to confirm the incorporation of Au-NPs in the PEDOT:PSS films. A solution mixing process was employed to incorporate the nanoparticles. When the diameter of the Au-NPs incorporated in the PEDOT:PSS films (Au-NPs/PEDOT:PSS) was 20 nm, the piezoresistive pressure sensing 2 × 2 arrays were almost immune to cross-talk effects, which enhances the pressure sensing accuracy of the array. The Au-NPs render the PEDOT:PSS films more resilient. This is confirmed by the high plastic resistance values using a nanoindenter, which reduce the interference between the active and passive cells. When the size of the Au-NPs is more than 20 nm, a significant cross-talk effect is observed in the pressure sensing arrays as a result of the high conductivity of the Au-NPs/PEDOT:PSS films with large Au-NPs. With the incorporation of optimally sized Au-NPs, the PEDOT:PSS piezoresistive pressure sensing arrays can be promising candidates for future high-resolution fingerprint identification system with multiple-electrode array structures.
- Published
- 2017
25. The long non-coding RNA LOC441204 enhances cell growth in human glioma
- Author
-
Cheng-Shian Tsai, Kwang-Huei Lin, I.-Hsiao Chung, Yin-Cheng Huang, Chang-Nen Chang, Wei-Jan Chen, Yu-Jen Lu, Tzu-Kang Lin, and Yang-Hsiang Lin
- Subjects
Male ,0301 basic medicine ,Science ,Biology ,Bioinformatics ,Article ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Glioma ,Biomarkers, Tumor ,Tumor Cells, Cultured ,medicine ,Humans ,beta Catenin ,Cell Proliferation ,Regulation of gene expression ,Gene knockdown ,Multidisciplinary ,Brain Neoplasms ,Cell growth ,Cell Cycle ,RNA ,Middle Aged ,Cell cycle ,Prognosis ,medicine.disease ,Long non-coding RNA ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,030220 oncology & carcinogenesis ,Proteolysis ,Cancer research ,Medicine ,Female ,RNA, Long Noncoding - Abstract
Glioma is the most common and aggressive type of brain tumor. While long non-coding RNAs (lncRNAs) are clearly more abundant in human brain than protein-coding genes, the specific roles of lncRNAs and mechanisms underlying their dysregulation in glioma remain unclear. Here, we focused on lncRNAs that are differentially expressed in brain tumor and their potential biological functions. LOC441204, a novel non-coding RNA gene displaying high expression in clinical specimens of brain tumor and significant upregulation in glioma cell lines in microarray analyses, was selected for further study. Notably, knockdown of LOC441204 suppressed tumor cell proliferation in two glioma cell lines. Moreover, LOC441204-induced tumor cell growth was mediated the stabilization of β-catenin pathway. Briefly, LOC441204 bound to β-catenin preventing its degradation, resulting in downstream p21 repression and cdk4 activation to enhance glioma cell proliferation. Collectively, our findings indicate a pro-oncogenic role of LOC441204 in tumor cell growth through activation of the β-catenin/p21/cdk4 cascade to act as a potential diagnostic marker or therapeutic target in brain tumor.
- Published
- 2017
26. Investigating the Association between Alcohol and Risk of Head and Neck Cancer in Taiwan
- Author
-
Shang Yin Wu, Cheng Chih Huang, Chun Yen Ou, Jeffrey S. Chang, Chen Lin Lin, Tung Yiu Wong, Sen Tien Tsai, Jang Yang Chang, Han Chien Yang, Wei Ting Hsueh, Sheen Yie Fang, Ya Ling Weng, Jehn Shyun Huang, Yao Chou Lee, Yu Cheng Lu, Yi Hui Wang, Yu Shan Chen, Chia Jui Yen, Chan Chi Chang, Jenn Ren Hsiao, Jiunn Liang Wu, Wei Ting Lee, Yuan Hua Wu, and Ken Chung Chen
- Subjects
0301 basic medicine ,Oncology ,Male ,medicine.medical_specialty ,Alcohol Drinking ,Genotype ,Taiwan ,lcsh:Medicine ,Alcohol ,Polymorphism, Single Nucleotide ,Risk Assessment ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Risk Factors ,Internal medicine ,medicine ,Odds Ratio ,Humans ,Genetic Predisposition to Disease ,lcsh:Science ,Alleles ,Multidisciplinary ,business.industry ,Head and neck cancer ,lcsh:R ,Case-control study ,ADH1B ,Hypopharyngeal cancer ,Odds ratio ,Middle Aged ,medicine.disease ,Surgery ,030104 developmental biology ,chemistry ,Head and Neck Neoplasms ,030220 oncology & carcinogenesis ,Case-Control Studies ,Population Surveillance ,Population study ,lcsh:Q ,Female ,Risk assessment ,business - Abstract
Although alcohol is an established risk factor of head and neck cancer (HNC), insufficiencies exist in the literature in several aspects. We analyzed detailed alcohol consumption data (amount and type of alcoholic beverage) of 811 HNC patients and 940 controls to evaluate the association between alcohol and HNC by HNC sites and by genotypes of ADH1B and ALDH2. Alcohol was associated with an increased HNC risk in a dose-response relationship, with the highest risk observed for hypopharyngeal cancer, followed by oropharyngeal and laryngeal cancers. Liquor showed a stronger positive association with HNC than beer and wine. The highest HNC risk occurred in individuals with the slow ADH1B and slow/non-functional ALDH2 genotype combination. In our study population, 21.8% of HNCs, 55.7% of oropharyngeal cancers, and 89.1% of hypopharyngeal cancers could be attributed to alcohol. Alcohol accounted for 47.3% of HNCs among individuals with the slow ADH1B and slow/non-functional ALDH2 genotype combination. The HNC risk associated with alcohol became comparable to that of never/occasional drinkers after ten or more years of cessation from regular alcohol drinking. In conclusion, alcohol use is associated with an increased HNC risk, particularly for individuals with slow ethanol metabolism. HNC incidence may be reduced by alcohol cessation.
- Published
- 2017
27. Heterogeneous Fenton Reaction Enabled Selective Colon Cancerous Cell Treatment
- Author
-
Kuan Ting Lee, Shao Chieh Chiu, Er Yuan Chuang, Wen Chi Chang, Shih-Yuan Lu, and Yu Jen Lu
- Subjects
0301 basic medicine ,inorganic chemicals ,Colorectal cancer ,Cell Survival ,lcsh:Medicine ,Metal Nanoparticles ,02 engineering and technology ,Ferric Compounds ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,medicine ,Organotin Compounds ,Humans ,Hydrogen peroxide ,lcsh:Science ,Cell Proliferation ,chemistry.chemical_classification ,Reactive oxygen species ,Multidisciplinary ,biology ,Chemistry ,lcsh:R ,Cancer ,Hydrogen Peroxide ,021001 nanoscience & nanotechnology ,medicine.disease ,Catalase ,030104 developmental biology ,Caco-2 ,Reagent ,Cancer cell ,Colonic Neoplasms ,biology.protein ,Cancer research ,lcsh:Q ,Caco-2 Cells ,0210 nano-technology ,Reactive Oxygen Species - Abstract
A selective colon cancer cell therapy was effectively achieved with catalase-mediated intra-cellular heterogeneous Fenton reactions triggered by cellular uptake of SnFe2O4 nanocrystals. The treatment was proven effective for eradicating colon cancer cells, whereas was benign to normal colon cells, thus effectively realizing the selective colon cancer cell therapeutics. Cancer cells possess much higher innate hydrogen peroxide (H2O2) but much lower catalase levels than normal cells. Catalase, an effective H2O2 scavenger, prevented attacks on cells by reactive oxygen species induced from H2O2. The above intrinsic difference between cancer and normal cells was utilized to achieve selective colon cancer cell eradication through endocytosing efficient heterogeneous Fenton catalysts to trigger the formation of highly reactive oxygen species from H2O2. In this paper, SnFe2O4 nanocrystals, a newly noted outstanding paramagnetic heterogeneous Fenton catalyst, have been verified an effective selective colon cancerous cell treatment reagent of satisfactory blood compatibility.
- Published
- 2016
28. Potassium Regulation in Medaka (Oryzias latipes) Larvae Acclimated to Fresh Water: Passive Uptake and Active Secretion by the Skin Cells
- Author
-
Horng, Jiun-Lin, primary, Yu, Li-Lu, additional, Liu, Sian-Tai, additional, Chen, Po-Yen, additional, and Lin, Li-Yih, additional
- Published
- 2017
- Full Text
- View/download PDF
29. Non-invasive screening for early Alzheimer’s disease diagnosis by a sensitively immunomagnetic biosensor
- Author
-
Chen-Chi M. Ma, Shan-Shan Li, Yu-Jen Lu, Chiung-Yin Huang, Hung-Wei Yang, Kuo-Chen Wei, Po Hung Hsu, Chih-Wen Lin, Sheng-Chi Lin, and Hao-Li Liu
- Subjects
Pathology ,medicine.medical_specialty ,Materials science ,02 engineering and technology ,Biosensing Techniques ,010402 general chemistry ,Immunomagnetic separation ,01 natural sciences ,Article ,Alzheimer Disease ,medicine ,Humans ,Mass Screening ,Mass screening ,Detection limit ,Reproducibility ,Multidisciplinary ,Amyloid beta-Peptides ,Immunomagnetic Separation ,Non invasive ,Reproducibility of Results ,021001 nanoscience & nanotechnology ,Peptide Fragments ,0104 chemical sciences ,Early Diagnosis ,Detection performance ,Magnetic nanoparticles ,0210 nano-technology ,Biosensor ,Biomedical engineering - Abstract
Amyloid-beta peptide 1–42 (Aβ42) is considered as a reliable biomarker for the early diagnosis of Alzheimer’s disease (AD). Thus, it is urgent to develop a simple and efficient method for the detection of Aβ42. In this work, a reusable biosensor based on magnetic nitrogen-doped graphene (MNG) modified Au electrode for the detection of Aβ42 has been developed. The antibodies of Aβ 1–28 (Aβab) are used as the specific biorecognition element for Aβ42 that were conjugated on the surface of MNG. In the presence of magnetic nanoparticles on MNG, the electrode coating material, the biosensor can be quickly constructed, without requiring an electrode drying process, which reduce the analysis time and is convenient for proceeding to detection. The reusable biosensor with good reproducibility and stability was linear within the range from 5 pg mL−1 to 800 pg mL−1, covering the cut-off level of Aβ42 and a detection limit of 5 pg mL−1 had been achieved. Furthermore, the fabricated biosensor for Aβ42 detection not only improves the detection performance but also reduces the cost and shortens the response time, demonstrating its potential in diagnosing applications.
- Published
- 2016
- Full Text
- View/download PDF
30. 3D-QSAR and docking studies of flavonoids as potent Escherichia coli inhibitors
- Author
-
Yu-Lin Lu, Ting Wu, Siyi Pan, Xixi Zang, XiaoJuan Qi, Xiaoyun Xu, and Yajing Fang
- Subjects
0301 basic medicine ,Steric effects ,Models, Molecular ,Quantitative structure–activity relationship ,Stereochemistry ,Quantitative Structure-Activity Relationship ,Bioinformatics ,01 natural sciences ,DNA gyrase ,Flavones ,Molecular Docking Simulation ,Article ,03 medical and health sciences ,Adenosine Triphosphate ,Escherichia coli ,heterocyclic compounds ,chemistry.chemical_classification ,Flavonoids ,Multidisciplinary ,Molecular Structure ,010405 organic chemistry ,Chemistry ,Hydrogen bond ,Escherichia coli Proteins ,Gene Expression Regulation, Bacterial ,0104 chemical sciences ,Anti-Bacterial Agents ,030104 developmental biology ,Docking (molecular) ,DNA Gyrase ,Antibacterial activity - Abstract
Flavonoids are potential antibacterial agents. However, key substituents and mechanism for their antibacterial activity have not been fully investigated. The quantitative structure-activity relationship (QSAR) and molecular docking of flavonoids relating to potent anti-Escherichia coli agents were investigated. Comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA) were developed by using the pIC50 values of flavonoids. The cross-validated coefficient (q2) values for CoMFA (0.743) and for CoMSIA (0.708) were achieved, illustrating high predictive capabilities. Selected descriptors for the CoMFA model were ClogP (logarithm of the octanol/water partition coefficient), steric and electrostatic fields, while, ClogP, electrostatic and hydrogen bond donor fields were used for the CoMSIA model. Molecular docking results confirmed that half of the tested flavonoids inhibited DNA gyrase B (GyrB) by interacting with adenosine-triphosphate (ATP) pocket in a same orientation. Polymethoxyl flavones, flavonoid glycosides, isoflavonoids changed their orientation, resulting in a decrease of inhibitory activity. Moreover, docking results showed that 3-hydroxyl, 5-hydroxyl, 7-hydroxyl and 4-carbonyl groups were found to be crucial active substituents of flavonoids by interacting with key residues of GyrB, which were in agreement with the QSAR study results. These results provide valuable information for structure requirements of flavonoids as antibacterial agents.
- Published
- 2016
31. Effects of the TLR4 signaling pathway on apoptosis of neuronal cells in diabetes mellitus complicated with cerebral infarction in a rat model
- Author
-
Li, Chao, primary, Che, Li-He, additional, Ji, Tie-Feng, additional, Shi, Lei, additional, and Yu, Jin-Lu, additional
- Published
- 2017
- Full Text
- View/download PDF
32. TLR signaling adaptor protein MyD88 in primary sensory neurons contributes to persistent inflammatory and neuropathic pain and neuroinflammation
- Author
-
Liu, Xing-Jun, primary, Liu, Tong, additional, Chen, Gang, additional, Wang, Bing, additional, Yu, Xiao-Lu, additional, Yin, Cui, additional, and Ji, Ru-Rong, additional
- Published
- 2016
- Full Text
- View/download PDF
33. Topological Quantum Phase Transition in Synthetic Non-Abelian Gauge Potential: Gauge Invariance and Experimental Detections
- Author
-
Sun, Fadi, primary, Yu, Xiao-Lu, additional, Ye, Jinwu, additional, Fan, Heng, additional, and Liu, Wu-Ming, additional
- Published
- 2013
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.