Search

Your search keyword '"Zhengrong Ding"' showing total 4 results
Start Over Author "Zhengrong Ding" Journal scientific reports
4 results on '"Zhengrong Ding"'

1. Complete Genome Analysis of an Enterovirus EV-B83 Isolated in China

Author

Jing-Jing Tang, Lin Lu, Kai Li, Bing-Jun Tian, Qiongfen Li, Jie Zhang, and Zhengrong Ding

Subjects
0301 basic medicine, Male, China, Sequence analysis, 030106 microbiology, Genome, Viral, Biology, medicine.disease_cause, Genome, Article, Cell Line, 03 medical and health sciences, Mice, medicine, Enterovirus Infections, Coding region, Animals, Humans, Child, Whole genome sequencing, Genetics, Multidisciplinary, Base Sequence, Sequence Analysis, RNA, Strain (biology), Virology, Enterovirus B, Human, Genetic divergence, Molecular Typing, 030104 developmental biology, GenBank, Enterovirus, RNA, Viral, Capsid Proteins
Abstract

Enterovirus B83 (EV-B83) is a recently identified member of enterovirus species B. It is a rarely reported serotype and up to date, only the complete genome sequence of the prototype strain from the United States is available. In this study, we describe the complete genomic characterization of an EV-B83 strain 246/YN/CHN/08HC isolated from a healthy child living in border region of Yunnan Province, China in 2008. Compared with the prototype strain, it had 79.6% similarity in the complete genome and 78.9% similarity in the VP1 coding region, reflecting the great genetic divergence among them. VP1-coding region alignment revealed it had 77.2–91.3% with other EV-B83 sequences available in GenBank. Similarity plot analysis revealed it had higher identity with several other EV-B serotypes than the EV-B83 prototype strain in the P2 and P3 coding region, suggesting multiple recombination events might have occurred. The great genetic divergence with previously isolated strains and the extremely rare isolation suggest this serotype has circulated at a low epidemic strength for many years. This is the first report of complete genome of EV-B83 in China.

Published
2016

2. Complete Genome Characterization of a Novel Enterovirus Type EV-B106 Isolated in China, 2012

Author

Wenbo Xu, Zhixian Zhao, Zhengrong Ding, Bing-Jun Tian, Jie Zhang, Yong Zhang, Jing-Jing Tang, Lifen Zhang, and Zexin Tao

Subjects
Genetics, China, Multidisciplinary, Phylogenetic tree, Strain (chemistry), Base Sequence, Proteome, Molecular Sequence Data, Chromosome Mapping, Genome, Viral, Biology, medicine.disease_cause, Genome, Virology, Article, Viral Proteins, Similarity (network science), Species Specificity, GenBank, medicine, Coding region, Enterovirus, Humans
Abstract

Human enterovirus B106 (EV-B106) is a recently identified member of enterovirus species B. In this study, we report the complete genomic characterization of an EV-B106 strain (148/YN/CHN/12) isolated from an acute flaccid paralysis patient in Yunnan Province, China. The new strain had 79.2-81.3% nucleotide and 89.1-94.8% amino acid similarity in the VP1 region with the other two EV-B106 strains from Bolivia and Pakistan. When compared with other EV serotypes, it had the highest (73.3%) VP1 nucleotide similarity with the EV-B77 prototype strain CF496-99. However, when aligned with all EV-B106 and EV-B77 sequences available from the GenBank database, two major frame shifts were observed in the VP1 coding region, which resulted in substantial (20.5%) VP1 amino acid divergence between the two serotypes. Phylogenetic analysis and similarity plot analysis revealed multiple recombination events in the genome of this strain. This is the first report of the complete genome of EV-B106.

Published
2014

3. Molecular Epidemiology and Recombination of Human Enteroviruses from AFP surveillance in Yunnan, China from 2006 to 2010.

Author

Jingjing Tang, Hiromu Yoshida, Zhengrong Ding, Zexin Tao, Jie Zhang, Bingjun Tian, Zhixian Zhao, and Lifen Zhang

Subjects
EPIDEMIOLOGY, ENTEROVIRUSES, ACUTE flaccid paralysis, MOLECULAR biology, NUCLEOTIDE sequence, DISEASE prevalence, JUVENILE diseases, DIAGNOSIS
Abstract

The study represents the genetic overview of non-polio enteroviruses (NPEV) isolated from acute flaccid paralysis (AFP) cases in Yunnan Province from 2006 to 2010. Molecular typing based on VP1 nucleotide sequence was carried out on 98 NPEV isolates, and 33 serotypes were identified. EV-B was detected most frequently with an overall prevalence of 71.4%, followed by EV-A (18.4%) and EV-C (10.2%). No EV-D was identified. NPEV positive rate was higher in children <3 years of age and in summer and autumn months. Clinically, 68.4% patients presented with fever, and 16 cases (16.3%) were classified as Guillain-Barré syndrome, followed by myositis (13.3%). The phylogenetic analysis on the VP1 and 3D regions of prevalent serotypes provided evidence for recombination events among them. EV-A71, an important pathogen previously demonstrated to be associated with paralysis, had also been detected (n=8) in this study and they all belonged to genotype C4. Great genetic divergence between Yunnan isolates and strains from other regions of the world was revealed. The findings of the study are of great importance for further research on molecular evolution of EV under the circumstance of no specialized EV surveillance system in China. [ABSTRACT FROM AUTHOR]

Published
2014
Full Text
View/download PDF

4. Complete Genome Characterization of a Novel Enterovirus Type EV-B106 Isolated in China, 2012.

Author

Jingjing Tang, Zexin Tao, Zhengrong Ding, Yong Zhang, Jie Zhang, Bingjun Tian, Zhixian Zhao, Lifen Zhang, and Wenbo Xu

Subjects
ENTEROVIRUS diseases, ENTEROVIRUSES, ACUTE flaccid paralysis, AMINO acids, SEROTYPES, GENETICS
Abstract

Human enterovirus B106 (EV-B106) is a recently identified member of enterovirus species B. In this study, we report the complete genomic characterization of an EV-B106 strain (148/YN/CHN/12) isolated from an acute flaccid paralysis patient in Yunnan Province, China. The new strain had 79.2-81.3% nucleotide and 89.1-94.8% amino acid similarity in the VP1 region with the other two EV-B106 strains from Bolivia and Pakistan. When compared with other EV serotypes, it had the highest (73.3%) VP1 nucleotide similarity with the EV-B77 prototype strain CF496-99. However, when aligned with all EV-B106 and EV-B77 sequences available from the GenBank database, two major frame shifts were observed in the VP1 coding region, which resulted in substantial (20.5%) VP1 amino acid divergence between the two serotypes. Phylogenetic analysis and similarity plot analysis revealed multiple recombination events in the genome of this strain. This is the first report of the complete genome of EV-B106. [ABSTRACT FROM AUTHOR]

Published
2014
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results