1. Improvement of thermal stability of proteins by evolutionary molecular engineering
- Author
-
Akihiko Yamagishi
- Subjects
Protein stability ,business.industry ,Mutant ,Substitution (logic) ,Thermal stability ,Computational biology ,Biology ,business ,Biotechnology ,Molecular engineering - Abstract
Thermal stability of proteins can be improved by evolutionary molecular engineering, without the knowledge about 3D structures of the proteins nor the principle that governs the protein stability. The method will provide a way of improving the thermal stability of industrially important proteins. It can also be used as a tool to study protein stability. There are a substantial number of examples now. Our recent study suggested that it is easy to obtain thermo-stabilized proteins by single-base substitution. Although, it requires further improvement of the method to obtain mutants with double-base substitution within a codon.
- Published
- 1996
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