1. A pin-based pyro-electrohydrodynamic jet sensor for tuning the accumulation of biomolecules down to sub-picogram level detection
- Author
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Simona Itri, Danila del Giudice, Martina Mugnano, Volodymyr Tkachenko, Sanna Uusitalo, Annukka Kokkonen, Inka Päkkilä, Heidi Ottevaere, Yunfeng Nie, Emanuela Mazzon, Agnese Gugliandolo, Pietro Ferraro, and Simonetta Grilli
- Subjects
Alzheimer's disease ,Aβ1-42 ,Biosensors ,P-jet ,Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
The detection of low abundant biomarkers is of great interest in different clinical cases, such as the early diagnosis of Alzheimer's disease. Here we present what we call pin-based pyro-electrohydrodynamic jet (p-jet) sensor that can linearly tune the accumulation rate of biomolecules in tiny droplets. In this newly revealed pin-based configuration we demonstrate the ability to detect biomolecules down to sub-picogram concentration. Two different fluorescent probes at a varying number of accumulated droplets, up to 50, have been used to investigate the p-jet sensor. Moreover, a comparative study with a commercial piezo-driven bio-spotter has been performed. The spots of biomolecules exhibit high reproducibility by achieving a coefficient of variation
- Published
- 2022
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