1. Real-time visualizing the regulation of reactive oxygen species on Zn2+ release in cellular lysosome by a specific fluorescent probe.
- Author
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Hu, Zhangjun, Yang, Guanqing, Hu, Jiwen, Wang, Hui, Eriksson, Peter, Zhang, Ruilong, Zhang, Zhongping, and Uvdal, Kajsa
- Subjects
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REACTIVE oxygen species , *ZINC ions , *LYSOSOMES , *FLUORESCENT probes , *CELLULAR signal transduction , *OXIDATIVE stress - Abstract
Reactive oxygen species (ROS) regulating the release of free zinc ions (Zn 2+ ) in cellular lysosome is closely related to various pathways of cellular signal transduction, such as inflammation and oxidative stress. Directly visualizing Zn 2+ release in lysosome is essential for in-depth understanding these physiological processes, and is still an atelic challenge. In this work, we successfully fabricate a lysosome-specific Zn 2+ fluorescent probe and achieve the visualization of ROS-induced Zn 2+ release in lysosome of inflammatory cells. The as-prepared probe combines a green fluorophore, an ionophore with five-dentate sites, and a morpholine as the lysosome-specific localization moiety. The fluorescence of the fluorophore in the free probe is suppressed by a photoinduced electron transfer (PET) process from nitrogen atoms in the ionophore. Upon the addition of Zn 2+ , the fluorescence can be promoted immediately, achieving the real-time detection. Meanwhile, the probe is sensitive and selective to Zn 2+ , which provides the capability to detect low-concentration of free Zn 2+ in lysosomes. Accordingly, the Zn 2+ release was clearly observed in lysosome with the increase of ROS levels when the inflammation occurred in living cells. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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