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Based on fluorescent resonance energy transfer (FRET), we developed a ratiometric fluorescent probe 1 for sensing Cu 2+ . As expected, probe 1 exhibited high selectivity and excellent sensitivity in both absorbance and fluorescence detection of Cu 2+ in aqueous solution. A significant color change from pale yellow to pink could be observed, enabling naked-eye detection of Cu 2+ . Furthermore, fluorescence imaging experiment of Cu 2+ in living MGC-803 cells demonstrated its value of practical applications in biological systems. [ABSTRACT FROM AUTHOR]

Photo-controlled method may improve the accuracy of fluorescent probes. Here, two new fluorescent probes HSP and MSP were designed based on naphthalimide and spiropyran. HSP exhibits a fluorescent emission at 540 nm after Human Serum Albumin (HSA) was added. Then with UV light irradiation, the spiropyran group of HSP is isomerized into the merocyanine, and the HSP/HSA hybrid displays red fluorescence emission at 610 nm, which demonstrats the probe have a unique fluorescence "double-check" mechanism to accurately detect HSA. As a comparison, a new probe MSP which structure similar to HSP was designed. Compared with HSP , the recognition effect of probe MSP on HSA is not obvious, which showed that the hydrophilic group hydroxyl displays an important influence on the response of the probe HSP to HSA. In addition, the HSP/HSA hybrid can also be used to detect SO 2 effectively. Most importantly, we have successfully applied HSP/HSA hybrid to image SO 2 in living cells. • The first case of hybrid by light-controlled method to identify SO 2. • The probe HSP can recognize HSA with "Double-Check" model. • Probe have good selectivity, low detection limit and avoid false positive interference. • The hydroxyl group of HSP is important and can bind to the amino acid residues of human serum albumin. [ABSTRACT FROM AUTHOR]

Two rhodamine based probes were designed and prepared for Sn 4+ via opening of the spiro-ring to give fluorescent and colored species. The two probes exhibited a strong fluorescence enhancement upon addition of Sn 4+ while showing almost no response to other cations in CH 3 CH 2 OH/HEPES buffer solution. Probe L 1 exhibited fast selectivity toward Sn 4+ and could be completed in 1 min. Fluorescence imaging experiments of Sn 4+ in living MGC803 cells demonstrated its value of practical applications in biological systems. [ABSTRACT FROM AUTHOR]