1. A double-cavity nor-seco-cucurbit[10]uril-based fluorescent probe for detection of ClO- and its application in cell imaging.
- Author
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Cen, Ran, Liu, Ming, Xiao, Han, Yang, Hai-Ping, Chen, Li-Xia, Li, Qiu, Wang, Cheng-Hui, Tao, Zhu, and Xiao, Xin
- Subjects
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CELL imaging , *FLUORESCENT probes , *INTRAMOLECULAR charge transfer , *BIO-imaging sensors , *OPTICAL properties , *DOUBLE bonds - Abstract
A supramolecular fluorescent probe based on double-cavity nor-seco -cucurbit[10]uril (ns -Q[10]) was constructed using the non-covalent host-guest interactions between ns -Q[10] and positively charged Astrazon Pink FG (APFG). The intramolecular charge transfer (ICT) effect induces the APFG@ ns -Q[10] probe to emit strong orange-red fluorescence. APFG@ ns -Q[10] exhibits desirable optical properties and low toxicity, and has been successfully applied to the imaging of HK2 and HeLa cells. When APFG@ ns -Q[10] interacts with ClO-, the C C double bond in APFG is oxidized and the molecule is cleaved, which leads to the fluorescence is quenched. The specificity and sensitivity of APFG@ ns -Q[10] to ClO- allows it to be used for the detection and tracking of ClO- in cells. APFG@ ns -Q[10] demonstrates good biocompatibility, which is an excellent foundation for its application in cell imaging. • A supramolecular fluorescent probe based on ns -Q[10])was constructed using the non-covalent host-guest interactions. • APFG@ ns -Q[10] exhibits desirable optical properties and low toxicity, and was applied to the cell imaging. • The specificity and sensitivity of APFG@ ns -Q[10] to ClO- allows it to be used for the detection of ClO- in cells. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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