1. A dual-signaling electrochemical ratiometric method for sensitive detection of carcinoembryonic antigen based on Au-Cu2S-CuS/graphene and Au-CeO2 supported toluidine blue complex.
- Author
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Wei, Yicheng, Ma, Hongmin, Ren, Xiang, Ding, Caifeng, Wang, Huan, Sun, Xu, Du, Bin, Zhang, Yong, and Wei, Qin
- Subjects
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BIOSENSORS , *CARCINOEMBRYONIC antigen , *GRAPHENE , *TOLUIDINE , *MESOPOROUS materials - Abstract
Developing electrochemical biosensor, particularly for high sensitive recognizing and detecting tumor marker at low level under benign conditions, is highly desired, but still remains a severe challenge. In this communication, we report a dual-signaling ratiometric method for sensitive detection of carcinoembryonic antigen (CEA) based on gold nanoparticles (Au NPs) functionalized Cu 2 S-CuS/graphene composite (Au-Cu 2 S-CuS/graphene) as the matrix material and carboxyl-Au nanoparticles (Au-COOH) functionalized mesoporous CeO 2 nanoparticles (Au-CeO 2 ) supported toluidine blue (TB) complex as signal label. The oxidation peak current of Cu 2 S-CuS decreases with the increase of CEA concentration, while the oxidation peak current of TB increases. The change of dual signals “Δ I = Δ I TB + |Δ I Cu2S-CuS |” (Δ I TB and |Δ I Cu2S-CuS | are the change values of the oxidation peak currents of TB and Cu 2 S-CuS, respectively) is used as the response signal for quantitative determination of CEA. Notably, the linear range is 0.001 ∼ 100 ng/mL with a detection limit of 0.78 pg/mL (S/N = 3). The ratiometric electrochemical system provides an extremely simple strategy for detecting tumor markers in a reliable, facile, sensitive, and specific manner, which has a promising application for quantitative detection of other tumor markers in clinical diagnosis. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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