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Highlights • A rapid-prototyping angle-scanning Surface plasmon resonance was presented. • FDM 3D printing technology was utilized to realize rapid prototyping and cost down manufacture. • Aptasensor was utilized to detect glycated hemoglobin (HbA1c) directly. • The linear detection response of HbA1c appeared in the range from 18 to 147 nM with a detection limit of 1 nM. Abstract Diabetes mellitus is one of the most common noncommunicable diseases in the world. Fasting blood glucose level is one of the diabetes diagnostic criteria, but it is readily affected by stress, exercise and many other factors. Glycated hemoglobin (HbA1c) is an important clinical index for diabetes and related diseases. In this study, a cheaper, rapid-prototyping, high-sensitivity angle-scanning surface plasmon resonance (SPR) was developed by fused deposition modeling (FDM) 3D printing technology for HbA1c detection. The sensor chip is based on an aptamer that has high affinity and high specificity to HbA1c. The results showed that this HbA1c-specific aptamer had high specificity for HbA1c, and the calculated K D was 6.13 × 10−8 M. The linear detection response of HbA1c appeared in the range of 18–147 nM, with a detection limit of 1 nM. This SPR HbA1c detection system could be a promising platform for developing clinical point-of-care diagnostic applications. [ABSTRACT FROM AUTHOR]

• Anti HCT interference blood glucose sensors based on fiber paper-based screen-printed carbon electrode were demonstrated. • The paper-based glucose sensor can reduce HCT interference significantly with HCT range 20% to 70%. • In consensus error grid, 95% of the data points lie within zones A and B. As the number of diabetic patients increases year by year, the demand for a self-monitoring blood glucose test also increases. Additionally, more in-depth studies of blood glucose biosensors have led to increasing accuracy requirements. However, at present, most enzyme glucose sensors have difficulty solving issues related to hematocrit (HCT) interference. Most studies on anti-HCT interference in blood glucose sensing have mainly focused on correction factors, such as glucose correction, and electrical impedance or resistivity characteristics to correct the impact of HCT. In this work, we developed an anti-HCT-interference blood glucose sensor based on a fiber paper-based screen-printed carbon electrode with a mediator (potassium ferricyanide) and glucose oxidase (GOD), which were fixed through inkjet printing. When testing whole blood samples with HCT levels of 20%–70% and a fluid dropping method, the results of a consensus error grid analysis showed that 91% of the data points lied within zones A and B. The glucose sensor was exclusively designed with a 16 μL chamber in the working area of the sensor. When the testing fluid was taken in by capillary sucking, the effect of HCT was further reduced, and 95% of the data points lied within zones A and B in the consensus error grid. [ABSTRACT FROM AUTHOR]