1. Multiplex paper-based electrochemical immunosensor for the simultaneous monitoring of blood biomarkers in Alzheimer's disease.
- Author
-
Gu, Jinyu, Wang, Liming, Zhao, Li, Zuo, Yingchun, Gao, Shuai, Gu, Hui, Wang, Yinan, and Yu, Yanyan
- Subjects
- *
ALZHEIMER'S disease , *MESOPOROUS silica , *ALPHA fetoproteins , *SILICA nanoparticles , *TRANSGENIC mice , *BIOLOGICAL assay - Abstract
Joint analysis of varied biomarkers in blood sample will provide more accurate results for the monitoring and management of Alzheimer's disease (AD). In addition to the known β-amyloid protein (Aβ) used as a reliable clinical marker for AD diagnosis, Fetuin B also demonstrates a strong correlation with AD progression. Especially, for medical diagnostics, on-site detection of multi-analyte is quite important and therefore, it is desirable to develop a rapid and portable, multiplex electrochemical assay for simultaneous detection of Aβ and Fetuin B, which has been seldomly reported before. To achieve this goal, a multiplex paper-based electrode was prepared via vacuum filtration, by which, single-walled carbon nanotubes (SWCNTs) and AuNPs were sequentially filtered through cellulose paper with a five-electrode pattern to form a conductive underlay and electrical connections. A nanocomposite of AuNPs-loaded dendritic mesoporous silica nanoparticles (dmSiO 2 -Au) was firstly synthesized and further deposited thionine (Thi) for signal amplification. An immunosensor was correspondingly fabricated, which involved a combination of multiplex electrodes and dmSiO 2 -Au-Thi nanocomposite to facilitate simultaneous monitoring of Aβo and Fetuin B. Additionally, ferrocene (F c) was applied onto a separate working electrode to perform a ratiometric electrochemistry for enhancing accuracy. The outcomes demonstrated high specificity, sensitivity, and stability, and no obvious cross-talking between three working electrodes. Application of this sensor resulted in a successful determination of Aβo and Fetuin B levels in serum and brain tissue of transgenic AD mice. Overall, the presented electrochemical immunosensors for performing multiplex diagnostics may facilitate to the exploration of more AD-related blood markers. • A multiplex paper-based electrode was prepared. • A sandwich-based immunosensor enabled simultaneous monitoring of Aβo and Fetuin B. • Ferrocene was applied onto electrode to construct a ratiometric electrochemistry. • Analysis of Aβo and Fetuin B in APP/PS1 transgenic AD mice was realized. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF