1. A mitochondrial-targeted fluorescent probe to sense pH and HOCl in living cells.
- Author
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Qin, Fengyun, Zhang, Yongru, Zhu, Jianming, Li, Yan, Cao, Wenbo, and Ye, Yong
- Subjects
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FLUORESCENT probes , *DETECTION limit , *FLUORESCENCE , *CELLS , *SOLUBILITY - Abstract
• A mitochondria-targetable "naked-eye" fluorescent probe RosPO(OH) 2 was obtained. • The first dual-function probe for simultaneous determination of HOCl and pH is reported. • Probe have rapid response, low detection limit and a wide range of pH application. • The fluorescent imaging demonstrated its value of practical application. A series of rosamine dyes were designed and synthesized through one-step reaction. Among them, bifunctional fluorescent probe RosPO(OH) 2 showed excellent water solubility and large molar extinction coefficient. RosPO(OH) 2 underwent a two-step ionization process as the pH value of the HEPES buffer increased, accompanied a blue shift of UV absorption and an enhancement of fluorescence intensity. This means it can be used as a pH sensor. After the addition of HOCl, the fluorescence of RosPO(OH) 2 was quenched and the color of the solution changed from pink to colorless which permitted "naked eye" detection. RosPO(OH) 2 could be used as a sensor for detecting HOCl with high selectivity, rapid response, low detection limit (52 nM) and a wide range of pH application (pH 1–10). Colocalization results showed that RosPO(OH) 2 could specifically stain in mitochondria. Due to the low toxicity and the excellent water solubility of RosPO(OH) 2 , it had been successfully applied to image pH and HOCl in living cells. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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