1. A Quantitative PCR-Electrochemical Genosensor Test for the Screening of Biotech Crops.
- Author
-
Moura-Melo, Suely, Miranda-Castro, Rebeca, de-los-Santos-Álvarez, Noemí, Miranda-Ordieres, Arturo J., Ribeiro dos Santos Junior, José, da Silva Fonseca, Rosana A., and Lobo-Castañón, María Jesús
- Subjects
TRANSGENIC organisms ,GENETICALLY modified foods ,NUCLEOTIDE sequence ,CHEMISORPTION ,MOSAIC viruses - Abstract
The design of screening methods for the detection of genetically modified organisms (GMOs) in food would improve the efficiency in their control. We report here a PCR amplification method combined with a sequence-specific electrochemical genosensor for the quantification of a DNA sequence characteristic of the 35S promoter derived from the cauliflower mosaic virus (CaMV). Specifically, we employ a genosensor constructed by chemisorption of a thiolated capture probe and p-aminothiophenol gold surfaces to entrap on the sensing layer the unpurified PCR amplicons, together with a signaling probe labeled with fluorescein. The proposed test allows for the determination of a transgene copy number in both hemizygous (maize MON810 trait) and homozygous (soybean GTS40-3-2) transformed plants, and exhibits a limit of quantification of at least 0.25% for both kinds of GMO lines. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF