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The detection and quantification of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) virus particles in ambient waters using a membrane-based in-gel loop-mediated isothermal amplification (mgLAMP) method can play an important role in large-scale environmental surveillance for early warning of potential outbreaks. However, counting particles or cells in fluorescence microscopy is an expensive, time-consuming, and tedious task that only highly trained technicians and researchers can perform. Although such objects are generally easy to identify, manually annotating cells is occasionally prone to fatigue errors and arbitrariness due to the operator's interpretation of borderline cases. In this research, we proposed a method to detect and quantify multiscale and shape variant SARS-CoV-2 fluorescent cells generated using a portable (mgLAMP) system and captured using a smartphone camera. The proposed method is based on the YOLOv5 algorithm, which uses CSPnet as its backbone. CSPnet is a recently proposed convolutional neural network (CNN) that duplicates gradient information within the network using a combination of Dense nets and ResNet blocks, and bottleneck convolution layers to reduce computation while at the same time maintaining high accuracy. In addition, we apply the test time augmentation (TTA) algorithm in conjunction with YOLO's one-stage multihead detection heads to detect all cells of varying sizes and shapes. We evaluated the model using a private dataset provided by the Linde + Robinson Laboratory, California Institute of Technology, United States. The model achieved a mAP@0.5 score of 90.3 in the YOLOv5-s6. [ABSTRACT FROM AUTHOR]

Currently, face recognition technology is the most widely used method for verifying an individual's identity. Nevertheless, it has increased in popularity, raising concerns about face presentation attacks, in which a photo or video of an authorized person's face is used to obtain access to services. Based on a combination of background subtraction (BS) and convolutional neural network(s) (CNN), as well as an ensemble of classifiers, we propose an efficient and more robust face presentation attack detection algorithm. This algorithm includes a fully connected (FC) classifier with a majority vote (MV) algorithm, which uses different face presentation attack instruments (e.g., printed photo and replayed video). By including a majority vote to determine whether the input video is genuine or not, the proposed method significantly enhances the performance of the face anti-spoofing (FAS) system. For evaluation, we considered the MSU MFSD, REPLAY-ATTACK, and CASIA-FASD databases. The obtained results are very interesting and are much better than those obtained by state-of-the-art methods. For instance, on the REPLAY-ATTACK database, we were able to attain a half-total error rate (HTER) of 0.62% and an equal error rate (EER) of 0.58%. We attained an EER of 0% on both the CASIA-FASD and the MSU MFSD databases. [ABSTRACT FROM AUTHOR]