17 results on '"Adil Denizli"'
Search Results
2. Metal-chelated magnetic nanoparticles for protein C purification
- Author
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Duygu Çimen, Nilay Bereli, and Adil Denizli
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Chemistry ,Process Chemistry and Technology ,General Chemical Engineering ,Nanoparticle ,Filtration and Separation ,02 engineering and technology ,General Chemistry ,010501 environmental sciences ,01 natural sciences ,Miniemulsion ,Metal ,020401 chemical engineering ,Polymerization ,visual_art ,visual_art.visual_art_medium ,Magnetic nanoparticles ,Chelation ,0204 chemical engineering ,0105 earth and related environmental sciences ,Nuclear chemistry - Abstract
In the study, magnetic poly(hydroxyethylmethacrylate-N-methacryloyl-(L)-histidine) (mPHEMAH) nanoparticles were prepared by miniemulsion polymerisation using N-methacryloyl-(L)-histidinemethylester...
- Published
- 2019
3. Simultaneous depletion of albumin and immunoglobulin G by using twin affinity magnetic nanotraps
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Sibel Büyüktiryaki, Lokman Uzun, Arzu Ersöz, Rıdvan Say, Adil Denizli, Anadolu Üniversitesi, Bitki, İlaç ve Bilimsel Araştırmalar Merkezi, Büyüktiryaki, Sibel, Say, Rıdvan, and Ersöz, Arzu
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General Chemical Engineering ,Sodium ,chemistry.chemical_element ,Filtration and Separation ,Igg Depletion ,02 engineering and technology ,01 natural sciences ,Immunoglobulin G ,Protein A Affinity Chromatography ,Gel electrophoresis ,Aqueous solution ,Chromatography ,Dye-Ligand Affinity Chromatography ,biology ,Chemistry ,Process Chemistry and Technology ,010401 analytical chemistry ,Albumin ,General Chemistry ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Covalent Conjugation ,Covalent bond ,Ionic strength ,biology.protein ,Albumin Depletion ,0210 nano-technology ,Protein A - Abstract
WOS: 000382372100015, For biomarker discovery, simultaneous removal of albumin and immunoglobulin G (IgG) become more important for both concentrating other species including potential biomarkers and getting rid of their masking effect. In this study, we have proposed a covalent and photosensitive cross-linking conjugation of the biomolecules on nanostructures for the depletion of target proteins from aqueous solution and serum. The effect of concentration, pH, temperature and ionic strength on the adsorption of twin affinity nanotraps based on Cibacron Blue F3GA and Protein A were investigated. The efficiency of albumin and IgG depletion from human serum was performed using sodium dodecylsulfate-polyacrylamide gel electrophoresis.
- Published
- 2016
4. Monolithic Boronate Affinity Columns for IgG Separation
- Author
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Nilay Bereli, Yeşeren Saylan, Lokman Uzun, and Adil Denizli
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Chromatography ,Chemistry ,Scanning electron microscope ,Process Chemistry and Technology ,General Chemical Engineering ,Composite number ,Filtration and Separation ,General Chemistry ,Methacrylate ,High-performance liquid chromatography ,chemistry.chemical_compound ,Polymerization ,medicine ,Swelling ,medicine.symptom ,Fourier transform infrared spectroscopy ,Boronic acid - Abstract
In this study, monolithic high performance liquid chromatography (HPLC) composite columns were synthesized for immunoglobulin G (IgG) separation by boronate affinity chromatography. 4-Vinyl phenyl boronic acid (VPBA) was polymerized with 2-hydroxyethyl methacrylate (HEMA). The poly(HEMA-VPBA) monoliths were crushed into fine particles by using ball-milling. Then, the crushed particles were embedded into poly(2-hydroxyethyl methacrylate) (PHEMA) cryogels to prepare monolithic HPLC composite columns. The PHEMA cryogel was also synthesized to evaluate the efficiency of the embedding process. The monolithic HPLC composite columns were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), surface area measurements, boron content analysis, swelling studies, and flow rate-back pressure relation. The performance of IgG separation of monolithic composite columns was determined with HPLC and the parameters such as pH, IgG concentration, temperature, flow rate, and ioni...
- Published
- 2014
5. Dye Attached Nanoparticles for Lysozyme Adsorption
- Author
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Murat Uygun, Deniz Aktaş Uygun, Sinan Akgöl, Canan Altunbaş, and Adil Denizli
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Aqueous solution ,Process Chemistry and Technology ,General Chemical Engineering ,technology, industry, and agriculture ,Emulsion polymerization ,Nanoparticle ,Filtration and Separation ,macromolecular substances ,General Chemistry ,chemistry.chemical_compound ,Adsorption ,Chemical engineering ,chemistry ,Covalent bond ,Polymer chemistry ,Fourier transform infrared spectroscopy ,Lysozyme ,Stoichiometry - Abstract
In this work, Reactive Blue 15 dye functionalized poly(HEMA) nanoparticles were synthesized for reversible adsorption of lysozyme from its aqueous solution. For this, nano-sized poly(HEMA) nanoparticles were synthesized by the surfactant free emulsion polymerization. Reactive Blue 15 dye then covalently attached to the polymeric structure. These novel dye attached poly(HEMA) nanoparticles were used for the adsorption of lysozyme. Characterization of dye attached nanoparticles was carried out by using FTIR, AFM, and elemental analysis. Incorporation of the dye onto the polymeric structure was demonstrated by FTIR and elemental analysis, while the size and the shape of the nanoparticles were shown by AFM. The incorporated amount of the dye was found to be 70.3 μmol/g nanoparticle with sulphur stoichiometry and it was found that the prepared nanoparticles were in a spherical form and were about 100 nm diameter. Lysozyme adsorption studies were carried out with different conditions (pH, lysozyme concentration...
- Published
- 2014
6. Histidine Containing Macroporous Affinity Cryogels for Immunoglobulin G Purification
- Author
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Adil Denizli, Nilay Bereli, and Gizem Ertürk
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Process Chemistry and Technology ,General Chemical Engineering ,Comonomer ,Radical polymerization ,Filtration and Separation ,General Chemistry ,(Hydroxyethyl)methacrylate ,chemistry.chemical_compound ,Adsorption ,chemistry ,Specific surface area ,Diamine ,Protein purification ,Polymer chemistry ,Ammonium persulfate - Abstract
A supermacroporous cryogel was prepared to obtain an efficient and cost effective purification of IgG from human plasma. N-methacryloyl-(L)-histidine methyl ester (MAH) was chosen as the pseudospecific ligand and/or comonomer. Poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methylester) [PHEMAH] cryogel was produced by free radical polymerization initiated by N,N,N′,N′-tetramethylene diamine (TEMED) and ammonium persulfate (APS) pair in an ice bath. PHEMAH cryogel had a specific surface area of 38.6 m2/g. PHEMAH cryogel was characterized by swelling studies, scanning electron microscopy and elemental analysis. PHEMAH cryogel containing 113.7 µmol MAH/g was used in the purification of IgG from human plasma. Compared with the poly(hydroxyethyl methacrylate) (PHEMA) cryogel (0.4 mg/g), the IgG adsorption capacity of the PHEMAH cryogel (24.7 mg/g) was improved significantly due to the MAH incorporation into the polymeric matrix. The maximum amount of IgG adsorption from aqueous solution in phospha...
- Published
- 2012
7. Copper Biosorption on Magnetically Modified Yeast Cells Under Magnetic Field
- Author
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Adil Denizli, Necdet Sağlam, Mirka Safarikova, Lokman Uzun, and Ivo Safarik
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inorganic chemicals ,biology ,Scanning electron microscope ,Process Chemistry and Technology ,General Chemical Engineering ,Metal ions in aqueous solution ,Saccharomyces cerevisiae ,Biosorption ,chemistry.chemical_element ,Filtration and Separation ,General Chemistry ,equipment and supplies ,biology.organism_classification ,Copper ,Yeast ,Metal ,chemistry.chemical_compound ,chemistry ,visual_art ,visual_art.visual_art_medium ,Perchloric acid ,human activities ,Nuclear chemistry - Abstract
Brewer's yeast (bottom yeast, Saccharomyces cerevisiae subsp. uvarum) cells were magnetically modified using water-based magnetic fluid stabilized perchloric acid. The magnetically modified yeast cells were characterized by scanning electron microscopy (SEM). Cu2+ biosorption properties of magnetically modified yeast cells from synthetic solutions were utilized in a continuous magnetic system. The Cu2+ ion-binding capacity decreased drastically with the increase of the flow rate. The maximum Cu2+ biosorption capacity was obtained to be 1.2 mmol/g at 25°C. Biosorption of Cu2+ increased with increasing pH and then reached almost a plateau value around pH 4.0. The yeast biomass can be easily regenerated by 0.1 M HNO3 with higher effectiveness. Biosorption of heavy metal ions from artificial wastewater was also studied. The biosorption capacities are 0.92 mmol/g for Cu2+, 0.52 mmol/g for Hg2+, and 0.28 mmol/g for Ni2+. Magnetically-modified yeast cells exhibits the following metal ion-affinity sequence: Cu2+ ...
- Published
- 2011
8. Poly(hydroxyethylmethacrylate‐N‐methacryloyl‐(L)‐histidine‐methyl‐ester) Based Metal‐Chelate Affinity Adsorbent for Separation of Lysozyme
- Author
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Adil Denizli, Tolga Çamlı, Begum Elmas, Müge Andaç, and Serap Şenel
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inorganic chemicals ,Ligand ,Process Chemistry and Technology ,General Chemical Engineering ,Comonomer ,Metal ions in aqueous solution ,Filtration and Separation ,General Chemistry ,Methacryloyl chloride ,chemistry.chemical_compound ,Adsorption ,chemistry ,Specific surface area ,Organic chemistry ,Suspension polymerization ,Lysozyme ,Nuclear chemistry - Abstract
Comonomer and/or metal‐chelating ligand N‐methacryloyl‐(L)‐histidine‐methylester (MAH) was synthesized by using methacryloyl chloride and L‐histidine methyl ester. Spherical beads with an average diameter of 75–125 µm were produced by suspension polymerization of 2‐hydroxyethyl methacrylate (HEMA) and MAH carried out in an aqueous dispersion medium. Poly(HEMA‐MAH) beads had a specific surface area of 18.3 m2/g. Elemental analysis of MAH for nitrogen was estimated as 895 µmol/g of polymer. Then the beads were loaded with different metal ions (i.e. Zn2+, Cu2+, Ni2+) to form the metal chelate. The effect of pH, concentration of lysozyme, and metal type on the adsorption of lysozyme to the metal‐chelated beads was examined in a batch reactor. Purification of lysozyme from egg‐white was also investigated. Maximum lysozyme adsorption capacity of poly(HEMA‐MAH) beads was found to be 8.7 mg/g at pH 7.0 in phosphate buffer. Lysozyme adsorption capacity of Zn2+, Cu2+, and Ni2+‐chelated beads was higher tha...
- Published
- 2004
9. Selective Separation of Uranium Containing Glutamic Acid Molecular-Imprinted Polymeric Microbeads
- Author
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Adil Denizli, Arzu Ersöz, Rıdvan Say, Anadolu Üniversitesi, Fen Fakültesi, Kimya Bölümü, Say, Rıdvan, and Ersöz, Arzu
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Lanthanide ,Process Chemistry and Technology ,General Chemical Engineering ,Metamorphic rock ,Inorganic chemistry ,chemistry.chemical_element ,Filtration and Separation ,General Chemistry ,Glutamic acid ,Uranium ,chemistry ,Monazite ,Organic chemistry ,Atomic number - Abstract
WOS: 000185542600004, …
- Published
- 2003
10. Cysteinylhexapeptide Attached Poly(2-Hydroxyethyl Methacrylate) Beads for Cd(II) Removal from Human Plasma in a Packed-Bed Column
- Author
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Cigˇdem Arpa, Ömer Genç, Sema Bektaş, Adil Denizli, and Handan Yavuz
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Packed bed ,Cadmium ,Process Chemistry and Technology ,General Chemical Engineering ,Ethylene glycol dimethacrylate ,chemistry.chemical_element ,Filtration and Separation ,General Chemistry ,(Hydroxyethyl)methacrylate ,2-Hydroxyethyl Methacrylate ,chemistry.chemical_compound ,Adsorption ,chemistry ,Human plasma ,Polymer chemistry ,Chemical stability - Abstract
Poly(hydroxyethyl methacrylate) (PHEMA) beads (in the size range of 150–200 μm) with good mechanical properties were prepared and crosslinked with ethylene glycol dimethacrylate (EGDMA) to increase their chemical stability. Because of their hydroxyl groups, they can serve as affinity adsorbent and can be employed for medical applications. Cibacron Blue F3GA was succesfully immobilized onto the beads. The maximum dye attachment was 16.5 μmol/g. Then, metallopeptide-ligand cysteinylhexapeptide (CysHP) was incorporated onto these beads and they were used for removal of cadmium ions [Cd(II)] from human plasma in a packed-bed column. The maximum amount of CysHP attached was 3.2 mg/g. Non specific Cd(II) adsorption from human plasma on the PHEMA beads was 0.32 mg/g. The adsorption capacity of the beads decreased from 11.8 to 3.7 mg/g with the raise of the flow-rate from 1.0 to 5.0 ml/min. It has been found that the CysHP loading has a great effect on the capacity of beads for adsorbing Cd(II) ions from human pl...
- Published
- 2003
11. Affinity separation of plasma proteins using a newly synthesized methacrylamidoalanine incorporated porous pHEMA membranes
- Author
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Yakup Arica, Adil Denizli, Rıdvan Say, Süleyman Patir, Handan Yavuz, Kırıkkale Üniversitesi, Anadolu Üniversitesi, Fen Fakültesi, Fizik Bölümü, and Say, Rıdvan
- Subjects
Chemistry ,Process Chemistry and Technology ,General Chemical Engineering ,Metal ions in aqueous solution ,Analytical chemistry ,Azobisisobutyronitrile ,Filtration and Separation ,General Chemistry ,Nuclear magnetic resonance spectroscopy ,Human serum albumin ,body regions ,chemistry.chemical_compound ,albumin adsorption ,Membrane ,Photopolymer ,Adsorption ,poly(HEMA) ,amino acid membranes ,metal chelates ,medicine ,Chelation ,Nuclear chemistry ,medicine.drug - Abstract
WOS: 000176800400005, In this study, we synthesized a novel adsorbent to obtain high protein-adsorption capacity utilizing 2-methacrylamidoalanine (MAAL) containing membrane. Amino acid-ligand MAAL was synthesized by using methacrylochloride and alanine. Then, poly(2-hydroxyethylmethacrylate-co-2-methacrylamidoalanine) [p(HEMA-co-MAAL)] membranes were prepared by UV-initiated photopolymerization of HEMA and MAAL in the presence of an initiator (azobisisobutyronitrile, AIBN). Synthesized MAAL was characterized by nuclear magnetic resonance spectroscopy. p(HEMA-co-MAAL) membranes were characterized by swelling studies, porosimeter, scanning electron microscopy, Fourier transform-infra red spectroscopy, and elemental analysis. These membranes have macropores in the size range 5-10 mum. Different metal ions including Zn(II), Ni(II), Co(II), and Cu(II) were chelated on these membranes. p(HEMA-co-MAAL) were used in the adsorption of human serum albumin (HSA) from aqueous media containing different amounts of albumin (0.1-5.0 mg L-1) and at different pH values (4.0-8.0). The maximum HSA adsorption was observed at pH 5.0. The nonspecific adsorption of HSA on the pHEMA membranes was negligible 0.9 mug cm(-2). MAAL incorporation significantly increased the HSA adsorption (1.76 mg cm(-2)). The HSA adsorption capacities of the metal-incorporated membranes were Greater than that of the p(HEMA-co-MAAL) membranes under the same conditions. Higher HSA adsorption capacity was observed from the human plasma (2.88 mg HSA cm(-2)).
- Published
- 2002
12. Bilirubin removal from human plasma by dye affinity microporous hollow fibers
- Author
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Fatma Denizli, Adil Denizli, Handan Yavuz, and Serap Şenel
- Subjects
Chromatography ,Bilirubin ,Process Chemistry and Technology ,General Chemical Engineering ,Sodium ,chemistry.chemical_element ,Filtration and Separation ,General Chemistry ,Microporous material ,chemistry.chemical_compound ,Membrane ,Adsorption ,chemistry ,Covalent bond ,Polyamide ,Sodium carbonate - Abstract
Bioaffinity adsorption has a unique and powerful role as a support tool in the removal of toxic substances from human plasma. Synthetic hollow-fiber membranes have advantages as support matrices in comparison to conventional hemoperfusion columns because they are not compressible and they eliminate internal diffusion limitations. In this study, Cibacron Blue F3GA was covalently attached onto commercially available microporous polyamide hollow-fiber membranes for bilirubin removal from hyperbilirubinemic human plasma. Different amounts of Cibacron Blue F3GA were attached on the polyamide hollow-fibers by changing the dye-attachment conditions, i.e., initial dye concentration, addition of sodium carbonate, and sodium chloride. The maximum amount of Cibacron Blue F3GA attachment was obtained at 42.5 μmol g−1 when the hollow fibers were treated with 3 M HCl for 30 min before performing the dye attachment. The nonspecific bilirubin adsorption on the unmodified polyamide hollow-fiber membranes was 0.65 mg g−1 f...
- Published
- 2002
13. CADMIUM (II) AND MERCURY (II) REMOVAL FROM AQUATIC SOLUTIONS WITH LOW-RANK TURKISH COAL
- Author
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Yuda Yürüm, Adil Denizli, Abdülkerim Karabakan, and Solmaz Karabulut
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chemistry.chemical_classification ,Cadmium ,Aqueous solution ,business.industry ,Process Chemistry and Technology ,General Chemical Engineering ,Metal ions in aqueous solution ,Carboxylic acid ,Inorganic chemistry ,technology, industry, and agriculture ,Langmuir adsorption model ,chemistry.chemical_element ,Filtration and Separation ,General Chemistry ,Mercury (element) ,symbols.namesake ,Adsorption ,chemistry ,symbols ,Coal ,business - Abstract
Removal of heavy metal ions from aqueous solutions containing low-to-moderate levels of contamination using Turkish Beypazari low-rank coal was investigated. Carboxylic acid and phenolic hydroxyl functional groups on the coal surface were the adsorption sites for heavy metal ions via the ion-exchange mechanism. The equilibrium pH of the coal-solution mixture was the principal factor controlling the extent of removal of Cd(II) and Hg(II) from aqueous solutions. The optimum pH was 4.0, and the adsorption reached equilibrium in 30 minutes. The maximum adsorption capacities of the metal ions from their single solutions were 1.55 mg for Hg(II) and 1.42 mg for Cd(II) per g of coal. Based on a weight uptake by coal, Hg(II) was found to have a greater affinity for the adsorption sites than does Cd(II). The same behavior was observed during competitive adsorption, that is, adsorption from binary solutions. The adsorption phenomena followed a typical Langmuir isotherm. The maximum adsorption capacities (q m) were c...
- Published
- 2001
14. SELECTIVE REMOVAL OF LEAD IONS BY POLYETHYLENE GLYCOL METHACRYLATE GEL BEADS CARRYING CIBACRON BLUE F3GA
- Author
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Ebru Büyüktuncel, Ömer Genç, Adil Denizli, and Ali Tuncel
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chemistry.chemical_classification ,Aqueous solution ,Polyethylene glycol methacrylate ,Chemistry ,Process Chemistry and Technology ,General Chemical Engineering ,Metal ions in aqueous solution ,Inorganic chemistry ,Filtration and Separation ,General Chemistry ,Polymer ,Cibacron Blue F3GA ,Ion ,Adsorption ,Stability constants of complexes - Abstract
Polyethylene glycol methacrylate (PEG-MA) gel beads carrying Cibacron Blue F3GA (42.6 μmol/g polymer) were prepared for the removal of Pb(II), Cu(II), and Cd(II) from aqueous solutions that contained different amounts of these ions (10–100 mg/L) and at different pH values (2.0–7.0). Adsorption rates were high, and adsorption equilibria were reached within 20 minutes. Adsorption of these metal ions on the unmodified PEG-MA gel beads was zero. The maximum adsorptions of heavy metal ions onto the Cibacron Blue F3GA–attached microbeads from single solutions were 23.3 mg/g (112.4 μmol/g) for Pb(II), 12.4 mg/g (110.3 μmol/g) for Cd(II), and 7.0 mg/g (110.2 μmol/g) for Cu(II). When the heavy metal ions competed (in the case of the adsorption from their mixture) the amounts of adsorption were 14.96 mg/g (72.2 μmol/g) for Pb(II), 0.72 mg/g (11.3 μmol/g) for Cu(II), and 1.10 mg/g (9.8 μmol/g) for Cd(II). Under competitive conditions, the system showed high selectivity for Pb(II) ions. The formation constants of Cib...
- Published
- 2001
15. HEAVY METAL SEPARATION CAPACITY OF A POROUS METHACRYLAMIDO-PHENYLALANINE CONTAINING MEMBRANE BASED ON A POLYHYDROXY-ETHYL METHACRYLATE MATRIX
- Author
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Süleyman Patir, Yakup Arica, Rıdvan Say, Adil Denizli, Anadolu Üniversitesi, Fen Fakültesi, Fizik Bölümü, Say, Rıdvan, and Kırıkkale Üniversitesi
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Nickel (Ii) ,Chemistry ,Process Chemistry and Technology ,General Chemical Engineering ,Azobisisobutyronitrile ,Filtration and Separation ,General Chemistry ,Nuclear magnetic resonance spectroscopy ,Methacrylate ,Copper (Ii) ,Mercury (Ii) ,chemistry.chemical_compound ,Monomer ,Membrane ,Photopolymer ,P(Hema-Co-Mapa) ,Heavy Metal Removal ,medicine ,Organic chemistry ,Swelling ,medicine.symptom ,Fourier transform infrared spectroscopy ,Nuclear chemistry - Abstract
WOS: 000171018800006, The abilities of various sorbent materials for heavy metal removal have been reported in the literature. We have developed a novel approach to obtain high metal-sorption capacity utilizing a membrane containing 2-methacrylamidophenylalanine. Metal-complexing ligand 2-methacrylamidophenylalanine (MAPA) was synthesized through the use methacrylo chloride and phenylalanine. Then, poly(2-hydroxyethyhmethacrylate-co-2-methacrylamidophenylalanine) (p(HEMA-co-MAPA)) membranes were prepared by UV-initiated photopolymerization of HEMA and MAPA in the presence of the initiator azobisisobutyronitrile. MAPA monomer was characterized by nuclear magnetic resonance spectroscopy. p(HEMA-co-MAPA) membranes were characterized by swelling studies, scanning electron microscopy, Fourier transform infrared spectroscopy, and elemental analysis. These membranes have large pores; the micropore dimensions are approximately 5-10 mum. p(HEMA-co-MA-PA) affinity membranes with a swelling ratio of 133.2% and containing 18.9 mmol MAPA/m(2) were used in the removal of the heavy-metal ions of copper, nickel, and mercury from aqueous media containing different amounts of these ions (5-600 mg/L) and at different pH values (2.0-7.0). The maximum adsorption capacities of heavy metal ions onto the MAPA-containing membranes under noncompetitive conditions were 23.8 = mmol/m(2) for Cu(II), 29.1 mmol/m(2) for Ni(II), and 50.3 mmol/m(2) for Hg(II). The affinity order was Hg(II) > Ni(II) > Cu(II). The adsorption of heavy metal ions increased with increasing pH and reached a plateau value at approximately pH 5.0. Adsorption of heavy metal ions from artificial wastewater was also studied. The adsorption capacities were 11.9 mmol/m(2) for Cu(II), 7.33 mmol/m(2) for Ni(II), and 9.79 mmol/m(2) for Hg(II). Desorption of heavy metal ions was performed using 0.1 M HNO3. The p(HEMA-co-MAPA) membranes are suitable for more than five cycles without noticeable loss of capacity.
- Published
- 2001
16. Performance of Different Metal–Dye Chelated Affinity Adsorbents of Poly(2-Hydroxyethyl Methacrylate) in Lysozyme Separation
- Author
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Adil Denizli, M. Yakup Arica, and Kırıkkale Üniversitesi
- Subjects
microporous membrane ,Aqueous solution ,Chemistry ,Process Chemistry and Technology ,General Chemical Engineering ,Metal ions in aqueous solution ,Inorganic chemistry ,Langmuir adsorption model ,Cibacron Blue F3GA ,Filtration and Separation ,General Chemistry ,Methacrylate ,affinity adsorption ,Dissociation constant ,symbols.namesake ,chemistry.chemical_compound ,Adsorption ,Membrane ,symbols ,Lysozyme ,lysozyme ,metal chelate ,Nuclear chemistry - Abstract
WOS: 000168274700005 The triazine dye Cibacron Blue F3GA was covalently immobilized as an affinity ligand onto microporous poly(2-hydroxyethyl methacrylate) (pHEMA) membranes. Three different metal ions [i.e.. Fe(III), Zn(II), or Cu(II)] were then chelated with the immobilized Cibacron Blue F3GA molecules. Lysozyme adsorption onto these affinity adsorbents from aqueous solutions containing different amounts of lysozyme at different pH was investigated in a batch system. Lysozyme adsorption capacity of all of the metal-dye-immobilized membranes was greater than that of the dye-immobilized membranes. The nonspecific adsorption of the protein on the pHEMA membranes was negligible. The adsorption phenomena appeared to follow a typical Lang muir isotherm. The maximum capacity (q(m)) of the Fe(III)-Zn(II),-or Cu(II)-dye chelated membranes for lysozyme adsorption (384, 326, and 306 mug/cm(2)) was greater than that of the dye-immobilized membrane (224 mug lysozyme/cm(2)), respectively. The dissociation constant (k(d)) values were found to be 2.51 x 10(-7) M with dye-immobilized membrane, and 2.32 X 10(-7), 2.38 X 10(-7), and 2.40 x 10(-7) M with the Fe(III)-Zn(II),-and Cu(II)-dye-chelated membranes, respectively. More than 95% of the adsorbed lysozyme was desorbed in 60 min in the desorption medium containing 0.5 M KSCN at pH 8.0.
- Published
- 2000
17. Congo Red-Attached Poly(EGDMA-HEMA) Micro beads for Removal of Heavy Metal Ions
- Author
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Erhan Pişkin, Bekir Sal¸h, and Adil Denizli
- Subjects
Vinyl alcohol ,Aqueous solution ,Chemistry ,Process Chemistry and Technology ,General Chemical Engineering ,Metal ions in aqueous solution ,Filtration and Separation ,General Chemistry ,(Hydroxyethyl)methacrylate ,Methacrylate ,Congo red ,chemistry.chemical_compound ,Adsorption ,Polymer chemistry ,Ethylene glycol - Abstract
In this study we investigated a new sorbent system, Congo Red-attached poly (EGDMA-HEMA) micro beads, for removal of heavy metal ions from aqueous solutions. Poly(EGDMA-HEMA) micro beads were prepared by suspension copolymerization of ethylene glycol di methacrylate (EGDMA) and hydroxyethyl methacrylate (HEMA) by using poly(vinyl alcohol), benzoyl peroxide, and toluene as the stabilizer, the initiator, and the diluent, respectively. Congo Red molecules were then covalently attached to these micro beads. Micro beads (150–200 o in diameter) with a swelling ratio of 55% and carrying 14.5 omol Congo Red/g polymer were used in the adsorption/desorption studies. Adsorption rate and capacity of the micro beads for selected metal ions, i.e., Cd(II), Cu(II), Zn(II) and Pb(II), were investigated in aqueous media containing different amounts of these ions (1–500 ppm) and at different pH values (1.5–7.5). Very high adsorption rates were observed at the beginning, and adsorption equilibria were then gradually...
- Published
- 1996
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