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Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the predictive value of serum plasminogen activator inhibitor-1(PAI-1) and retinol-binding protein 4(RBP4) levels for patients with acute ST-segment elevation myocardial infarction (STEMI) with‐ out reflow after percutaneous coronary intervention (PCI). Methods Totally 129 STEMI patients were selected as the STEMI group. According to the blood flow grading of thrombolysis therapy after PCI, they were divided into the no-reflow group (38 cases) and the reflow group (91 cases), and 55 healthy subjects were selected as the control group during the same period. Clinical data of STEMI patients were collected, and serum levels of PAI-1 and RBP4 were detected by en‐ zyme-linked immunosorbent assay. The predictive value of serum PAI-1 and RBP4 levels in patients with STEMI without reflow after PCI was analyzed by receiver operating characteristic (ROC) curve. Results Compared with the control group, the serum levels of PAI-1 and RBP4 in STEMI group increased (all P<0. 05). Compared with the reflow group, the serum levels of PAI-1 and RBP4 in no-reflow group increased (all P<0. 05). Multivariate Logistic regression analysis showed that the increase of age and the increase of LDL cholesterol, PAI-1 and RBP4 were independent risk factors for noreflow after PCI in STEMI patients (all P<0. 05), and the increase of left ventricular ejection fraction was a protective fac‐ tor (P<0. 05). ROC curve analysis showed that the area under the curve of serum PAI-1 and RBP4 levels was greater than that of PAI-1 and RBP4 alone in predicting no-reflow of STEMI patients after PCI (both P<0. 05). Conclusion The in‐ creased serum levels of PAI-1 and RBP4 in STEMI patients are associated with no-reflow after PCI, and the serum PAI-1 combined with RBP4 has a high value in predicting no-reflow after PCI. [ABSTRACT FROM AUTHOR]

Objective To investigate the effects and mechanism of Taoren Honghua decoction on the gestational diabetes mellitus（GDM）rats and the outcomes of their offspring. Methods Forty SPF SD female rats and 80 SPF SD male rats were selected. Forty female rats were randomly divided into groups A，B，C and D，with 10 rats in each. During the estrous period，female and male rats were caged at the ratio of 1：2，and the pregnancy of female rats was confirmed by vaginal smear method. On the 5th day of pregnancy，rats in the group B，group C and group D were injected with STZ in caudal vein to prepare GDM model. Rats in the group A were injected with equal volume of citrate-disodium hydrogen phosphate buffer，rats in the group C were given Taoren Honghua decoction 10 g/kg intragastrically on the modeling day，rats in the group D were given metformin 200 mg/kg intragastrically；rats in the groups A and B were given the same volume of normal saline，once a day，for 19 days. Fasting blood glucose（FPG）and insulin were measured by ELISA before pregnancy and on day 7 and 19 of pregnancy，respectively. After anesthesia on day 19 of gestation，the embryos were extracted and separated by laparotomy. The number of embryos in each group was calculated，and the amount of fetal body fat at birth was measured. The pathological changes of placental tissues were observed by HE staining，and the mRNA expression of insulin-like growth factor 2mRNA binding protein 2（IGF2BP2）and nuclear factor κB（NF-κB）in the placental tissues were detected by qRT-PCR. The protein expression levels of IGF2BP2 and NF-κB in placental tissues were detected by Western blotting. Results On day 7 and 19 of pregnancy，the levels of FPG and insulin in the group B were higher than those in the group A（all P<0. 05），and the above indexes in the group C were lower than those in group B（all P< 0. 05）. Compared with the group A，group B had lower embryo survival and birth body fat（both P<0. 05）. Compared with the group B，group C had higher embryo survival and birth body fat（both P<0. 05）. The placental tissues of group A were normal. In the group B，the placental tissue layer was blurred，the intercellular space was enlarged，and the cell layout was disordered. The pathological changes of placenta tissues in the groups C and D were lighter than those in the group B. Compared with the group A，the mRNA and protein expression levels of IGF2BP2 and NF- κB in the group B were higher（all P<0. 05）. Compared with the group B，the expression of the above indexes in group C was lower（all P< 0. 05）. There were no statistically significant differences in the above indexes between group C and group D（all P> 0. 05）. Conclusions Taoren Honghua decoction can reduce blood glucose and insulin levels，increase the survival rate and body fat amount of offspring embryos，and improve placental tissue in GDM rats during pregnancy. The mechanism may be related to inhibiting the activation of IGF2BP2 and NF-κB signaling pathways. [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the effects of isoalantolactone on proliferation and apoptosis of human bladder cancerT2 4 cells and its mechanism. Methods CDT2 4 cells in the logarithmic phase were divided into the groups 1, 2, 3, 4, 5 and 6; cells in the groups 1-5 (experimental group) were added with different concentrations of isoalantolactone (5, 10, 20, 40, and 80 μmoVL), and group 6 with normal culture medium (control group) ; after 24, 48, 72 h of culture, the cell proliferation ab山ty of each group was detected by C C K-8 method. (2) T24 cells were divided into the groups A, B, C, D, and E; cells in the group A were cultured in medium without isoalantolactone, cells in the groups B and E were pretreated with 5 nmol/L N-acetylcysteine (NAC ) for 2 h, and cells in the groups C, D and E were cultured with 10, 20 and 20 μmol/L isoalantolactone; after 24 hours of culture, the apoptosis rate of each group was measured by flow cytometry, the level of reactive oxygen species (ROS) was detected by DC FH-DA, and the mitochondrial membrane potential was detected by JC-1 mitochondrial membrane potential. @ T24 cells were divided into 4 groups with 6 compound wells in each group; cells in the three groups were added with 10, 20 and 40 μ,moVL isoalantolactone, and cells in the other group were treated with culture medium without isoalantolactone (control group) ; after 24 hours of culture, Western blotting was used to detect apoptosis-related proteins Bax, B-lymphocyticoma-2 (Bcl-2), Cytochrome C, and Caspase-3. Results (D Compared with the group 1, the inh山山on rate of cell proliferation increased in the groups 2, 3, 4 and 5 (all P < 0. 05) ; compared with the group 2, the inhibition rate of cell proliferation increased in the groups 3, 4 and 5 (all P < 0. 05) ; compared with the group 3, the inh如tion rate of cell proliferation increased in the groups 4 and 5 (all P < 0. 05). @ Compared with the group A, the apoptosis rate and ROS content increased in the groups C and D (all P < 0. 05) ; compared with the group C, the apoptosis rate and ROS content increased in the group D (all P < 0. 05) ; compared with the group D, the apoptosis rate and ROS content were reduced in the group E (all P < 0. 05). Compared with the group A, the mitochondrial membrane potential of group C decreased (P < 0. 05). Compared with the group C, the mitochondrial membrane potential of group D decreased (P < 0. 05) ; compared with the group D, the cell mitochondrial membrane potential increased in the group E (P < 0. 05). @ Compared with the control group, the relative expression levels of Bax, Cytochrome C and Caspase-3 in the drug groups increased, and the relative expression levels of Bcl-2 decreased (all P <0 . 05); with the increase of concentration, the relative expression levels of Bax, Cytochrome C and Caspase-3 in the drug group increased, and the relative expression levels of Bcl-2 decreased (all P <0. 05). Co nclusion lsoala ntolactone can inhibit cell proliferation and induce apoptosis by promoting the accumulation of reactive oxygen specie, decreasing the mitochondrial membrane potential and regulating the expression of apoptosis-related proteins [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)