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Objective To analyze the quality and diagnostic performance of images of dual-color fluorescence staining of human glioma and to compare dual-color fluorescence staining with HE staining. Methods Seventy-one cases of fresh human brain tissue specimens were collected and divided into two samples of the same size for dual-color fluorescence staining and HE staining. The lipids and DNA of brain tissues in one sample were stained with BODIPY and DRAQ5, respectively, and fluorescence imaging was performed under laser scanning confocal microscope, and we collected the fluorescence images. The fluorescence images were randomly evaluated by two pathologists and were compared with the images of HE staining. Another sample was stained with HE and scanned with a slide scanner. Two pathologists evaluated the images of dual-color fluorescence staining and HE staining separately after staining and evaluated again after 90 days of dualcolor fluorescence staining. The consistency test was performed on the result of evaluation of the two pathologists and κ value was calculated to judge the diagnostic consistency of dual-color fluorescence staining and HE staining. The diagnostic accuracy rate, sensitivity, and specificity of dual-color fluorescence staining were calculated. Results The fluorescence images of dual-color fluorescence staining of human glioma showed similar histological characteristics to images of HE staining, and lipids of dual-color fluorescence staining were remained complete. Pathologist A obtained 21 cases of lowgrade glioma and 50 cases of high-grade glioma by evaluating the images of dual-color fluorescence staining. Pathologist B obtained 24 cases and 47 cases, respectively. Kappa consistency test was performed between the first diagnosis results of pathologists A and B and the diagnosis results of HE staining, and κ value was 0. 48 and 0. 64, respectively. After 90 days of dual-color fluorescence staining, two pathologists evaluated the images of dual-color fluorescence staining for the second time. In this time, pathologist A obtained 26 cases of low-grade glioma and 45 cases of high-grade glioma and pathologist B evaluated 28 and 43 cases, respectively. Kappa consistency test was performed by comparing the second diagnosis result of pathologists A and B with the diagnosis result of HE staining, and κ values were 0. 76 and 0. 82, respectively. The sensitivity, specificity, and diagnostic accuracy of pathologist A were 88%, 57% and 76% in the first diagnosis, and 93%, 82% and, 89% in the second diagnosis, respectively. The sensitivity, specificity and accuracy of pathologist B were 91%, 71%, and 83% in the first diagnosis, and 93%, 89% and 92% in the second diagnosis, respectively. Conclusions The images of dual-color fluorescence staining of human glioma have similar histological characteristics to images of HE staining, and dual-color fluorescence staining remains lipids complete. Compared with HE staining, the diagnostic consistency of dual-color fluorescence staining is basically satisfied and it has high diagnostic accuracy, sensitivity, and specificity. [ABSTRACT FROM AUTHOR]

Objective To explore the expression levels of serum human cartilage glycoprotein 39 (YKL-40) m patients with psoriasis vulgaris and its clinical significance. Methods A total of 109 patients with psoriasis vulgaris ( the case group) were divided into the mild group ( n =52), moderate group ( n = 37), and severe group (n = 20) according to the psoriasis area and severity index ( PASI) . At the same time, 60 healthy volunteers were randomly selected as the co ntrol group. Serum YKL-40 and inflammatory cytokines such as IL-17, IL-21, IL-22, IL-23 levels were detected by enzymelinked immunosorbent assay. Pearson product moment correlation analysis was used to analyze the associations of serum YKL-40 with PASI, IL-17, IL-21, IL-22, and IL-23. We drew the receiver operating characteristic (ROC ) curve and calculated the area under the ROC curve ( AUC ) to evaluate the diagnostic value of serum YKL-40 for psoriasis vulgaris. Results The serum levels of YKL-40, IL-17, IL-21, IL-22 and IL-23 in the case group were higher than those in control group (all P < 0. 05) . With the severity of psoriasis vulgaris, the levels of serum YKL-40, IL-17, IL-21, IL-22, IL-23 increased gradually (all P < 0. 05) . The serum YKL-40 was positively correlated with PASI score ( r = 0. 792, P < 0. 05), and was positively correlated with inflammatory cytokines such as IL-17, IL-21, IL-22, IL-23 (r = 0. 751, 0. 783, 0. 811, 0. 765; all P < 0. 05 ) . The AUC of YKL-40 in the diagnosis of psoriasis vulgaris was 0. 938 (95% CI: 0. 893-0. 983), the cut -off value was 56.82 fLg/ml, the sensitivity and specificity were 0. 89 and 0. 92, respectively, and the accuracy was 0. 93. Conclusions YKL-40 increases in the pathogenesis of psoriasis vulgaris, which can reflect the severity of the disease and participate in the inflammatory response. Early detection can be used as marker for clinical diagnosis of psoriasis vulgaris. [ABSTRACT FROM AUTHOR]

Objective To investigate the effects of CD151 and its integrin binding mutant ( CD151-mut) on the invasion of hepatoma cells and its relationship with FAK-Src-paxillin signaling pathway. Methods HepG2 cells were randomly divided into the control group, CD151 group and CD151-mut group. Cells in the control group were added with normal saline, while cell in the CD151 group and CD151-mut group were transfected with pAAV -CD151 and pAAV -CD151- AAA plasmids, respectively, and then were incubated for 4 hours. After 24 hours of continuous culture, the cells were collected, and the invasive ability in each group was observed by Transwell chamber experiment. The activities of CD151 and FAK-Src-paxillin pathway were detected by Western blotting, and the CD151, CD151-mut and integrin α3, α6, β1 were detected by immunocoprecipitation. Results The number of transmembrane cells in the CD151 group was higher than that in the control group, and that in the CD151-mut group was lower than that in the CD151 group (both P < 0. 05). The expression of CD151 protein in the CD151 group and CD151-mut group was higher than that in the control group ( P < 0.05). The expression levels of p-FAK, p-Src and p-paxillin in the CD151 group were higher than those in the control group, while the expression levels of p-FAK, p-Src, and p-paxillin in the CD151-mut group was lower than those in the CD151 group. The results of immunocoprecipitation showed that CD151 combined with integrin a3, a6 and [31 to form complexes. The levels of integrin α3, α6 and β1 in the CD151 group were higher than those in the control group (all P < 0. 05), while the levels of integrin α3, α6 and β1 in the CD151-mut group were lower than those in the CD151 group (all P < 0. 01). Conclusion The overexpression of CD151 can promote the invasion of hepatoma cells, and the destruction of CD151 and integrin complex will weaken the invasion of hepatoma cells, which is related to the regulation of the activity of F AK-Src-paxillin signaling pathway protein. [ABSTRACT FROM AUTHOR]

目的观察寻常性银屑病患者血清内皮细胞特异性分子(ESM-1)、下丘脑抑食因子nesfatin-1的表达变化,并探讨其临床意义。方法 102例寻常性银屑病患者为观察组,同期60例健康体检者为对照组,收集两组空腹静脉血,ELISA法检测两组血清ESM-1、nesfatin-1,评估观察组患者皮损面积与严重度指数(PASI),Pearson积矩相关分析血清ESM-1、nesfatin-1水平与寻常性银屑病患者PASI的相关性,绘制受试者工作特征曲线(ROC)评估血清ESM-1、nesfatin-1水平对寻常性银屑病的诊断价值。结果治疗前、后观察组血清ESM-1分别为(2. 64±0. 89)、(1. 93±0. 81)μg/L,血清nesfatin-1分别为(0. 08±0. 04)、(0. 15±0. 04)μg/L。对照组血清ESM-1、nesfatin-1水平(1. 89±0. 75)、(0. 16±0. 03)μg/L。与治疗前比较,治疗后观察组血清ESM-1水平降低、nesfatin-1水平升高,PASI评分降低(P均<0. 05)。与对照组比较,治疗前观察组血清ESM-1水平高,nesfatin-1水平降低(P均<0. 05)。治疗前、后观察组PASI评分分别为(43. 75±5. 42)、(6. 68±6. 01)分,二者比较,P <0. 05。血清ESM-1水平与寻常性银屑病患者PASI评分呈正相关(r=0. 739,P <0. 05),血清nesfatin-1水平与寻常性银屑病患者PASI评分呈负相关(r=-0. 681,P <0. 05)。血清ESM-1水平诊断寻常性银屑病的ROC下面积(AUC)为0. 790,最佳截断值为2. 19μg/L时,低于2. 19μg/L的血清ESM-1水平诊断寻常性银屑病的灵敏度、特异度、准确度分别为82%、79%、78%;血清nesfatin-1水平诊断寻常性银屑病的AUC为0. 801,最佳截断值为0. 09μg/L,高于0. 09μg/L的血清nesfatin-1水平诊断寻常性银屑病的灵敏度、特异度、准确度分别为77%、75%、77%。结论寻常性银屑病患者血清ESM-1水平降低、nesfatin-1水平升高。血清ESM-1、nesfatin-1二者呈负相关,可能在寻常性银屑病的发生、发展中发挥重要作用。血清ESM-1、nesfatin-1水平诊断寻常性银屑病的灵敏度、特异度均较高。 [ABSTRACT FROM AUTHOR]

目的 探讨纳洛酮配伍曲马多对炎性疼痛大鼠的镇痛作用及机制。方法 将雄性SD大鼠随机分为空 内组、模型组、曲马多组、纳洛酮组、低剂量纳洛酮+曲马多组（Nl+T)组、高剂量纳洛酮+曲马多组（NH+T)组，每 组12只;后五组建立甲醛炎性疼痛大鼠模型后，分别鞘内注射生理盐水、曲马多、纳洛酮、不同剂量纳洛酮和曲马 多。记录给药后60 min内（每隔5 min)注射侧后足的疼痛评分;建模后1 h采用免疫组化法和Western blotting法 检测各组大鼠脊髓背角c-FoS蛋内表达的平均光密度（AOD)值和相对光密度（ROD)值结果在大鼠足底注射 甲醛10 min后的各观察时间点疼痛评分:模型组、纳洛酮组 > 曲马多组> NH + T组> NL + T组 > 空白组,组间比 较P均<0.01;纳洛酮组与模型组比较差异均无统计学意义（P均>0. 05)。脊髓背角c-Fos阳性产物AOD值及 ROD值:模型组高于空内组及曲马多组,NL+ T和NH + T组低于曲马多组,NH + T组低于NH + T组,组间比较P均 <0.01。结论小剂量纳洛酮能促进曲马多对炎性疼痛的镇痛作用，其机制与下调脊髓背角c-Fos蛋内表达有关。 [ABSTRACT FROM AUTHOR]

目的　观察不同剂量曲马多对炎症性疼痛大鼠疼痛行为学的影响，并探讨其可能机制。方法　60 只雄 性SD 大鼠随机分为5 组各12 只，TH、TM、TL、F 组在大鼠左足后底注射5％甲醛100 μL 制备炎症性疼痛模型，NS 组注射等量生理盐水。10 min 后，TH、TM、TL 组大鼠鞘内分别注射25、15、5 μg/ kg 曲马多（溶于20 μL 生理盐水）， F、NS 组分别注射等量生理盐水。比较各组大鼠疼痛行为学评分及脊髓背角P2X7 受体阳性细胞数、P2X7 受体蛋 白ROD 值。结果　与NS 组比较，F 组制模后5 ～60 min 疼痛行为学评分增加；与F 组比较，TL 组制模后15 ～ 60 min、TM 组制模后10 ～60 min、TH 组制模后5 ～60 min 疼痛行为学评分减少；与TL 组比较，TM 组、TH 组制模后10 ～60 min 疼痛行为学评分减少；与TM 组比较，TH 组制模后10 ～ 60 min 疼痛行为学评分减少；P 均< 0． 05。与NS 组比较，F 组P2X7 受体阳性细胞数、P2X7 受体蛋白ROD 值增加；与F 组比较，TH、TM、TL 组P2X7 受体阳性细胞 数、P2X7 受体蛋白ROD 值减少；与TL 组比较，TH、TM 组P2X7 受体阳性细胞数、P2X7 受体蛋白ROD 值减少；与 TM 组比较，TH 组P2X7 受体阳性细胞数、P2X7 受体蛋白ROD 值减少；P 均< 0． 05。结论　曲马多对炎症性疼痛 大鼠有镇痛效应，且呈剂量依赖性，高剂量镇痛效果更好，机制可能与其可下调脊髓背角P2X7 受体表达有关。 [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the effects of hypoxia inducible factor lex ( HIF- 1α) on the invasion and migration of human choriocarcinoma JEG- 3 cells under hypoxia. Methods JEG- 3 cells in the logarithmic phase were divided into groups A, B, G, and D;and cells in thegroups A,B, and G were cultured under hypoxia in vitro, but the group D was not treated. When JEG- 3cells were fusedto 60% to70% , cells in the groupsA and 6 were transfected with HIF- 1α siRNA and NG siRNA, respectively, and we did not do the treatment on the cells in the groups B and D. Real- time PGR and Western blotting were used to detect the mRNA and protein expression levels of HIF- 1α and GXGR4 in each group, respectively. The invasion and migration abilities of JEG- 3 cells were separately analyzed by Transwell invasion assay and Scratch test. Results The invasion and migration abilities of JEG- 3 cells in the group A were significantly lower than those in the other 3 groups (all P <0.05) , the invasion and migration abilities of JEG- 3 cells in the groups B and G were higher than those in the group D (all P<0.05). The mRNA and protein expression levels of HIF- 1α and GXGR4 in the group A were significantly lower than those in the other 3 groups (all P < 0. 05 ) , while the mRNA and protein expression levels of HIF- 1α and GXGR4in the groups Band G were significantly higher thanthose in the group D ( all P <0. 05 ). Conclusion Silencing HIF-1α can inhibit the invasionand migration abilities ofof JEG- 3 cells under hypoxia, and its molecu1αr mechanism may be re1αted to the down-regu1αtion of GXGR4. [ABSTRACT FROM AUTHOR]

Klippel-Feil综合征（KFS)是一种先天性疾病，常伴有颈椎椎体融合畸形，临床表现为典型的三联征:短 颈、颈部活动受限和低发际线。KFS的诊断需要借助影像学检查，优先选择颈椎X线检查;对于不能明确诊断或需 鉴别诊断时，可行多层螺旋CT或核磁共振检查。KFS的手术治疗方式有后路切除减压或椎板成形术、常规后路减 压钉棒内固定术、前路切开减压和植骨钢板内固定术、内镜经前方人路减压和（或）内固定术、人T.颈椎间盘置换术 和导航辅助内固定术等，不同手术方式的适用范围不同，各有优缺点。 [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)