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Objective To investigate the effect of moxibustion on the expression of Caveolin-1, p38MAPK, and pp38MAPK in the articular cartilage of knee osteoarthritis (KOA) rabbits. Methods Forty rabbits were randomly divided into the control group, model group, moxibustion group, and blocker group. Except the control group, KOA models were established by injecting papain into articular cavities of rabbits in the other three groups. Moxibustion was carried out at Dubi point and Zusanli point in the moxibustion group, and p38 blocker S8203580 was injected into articular cavities of rabbits in the blocker group. Rabbits were sacrificed after 3 weeks of treatment to obtain the cartilage tissues, while the rabbits in the control group and model group were not treated. The expression levels of Caveolin-1, p38 MAPK, and p38 MAPK were detected by Western blotting. Results The relative expression levels of Caveolin-1 were 2. 09 ± 0. 07, 1. 45 ± 0. 05, and 1. 09 ± 0. 07, respectively, and the relative expression levels of p-p38 MAPK/p38 MAPK were 3. 25 ± 0. 08, 2. 52 ± 0. 17, and 2. 07 ± 0. 14, respectively, in the model group, moxibustion group and inhibitor group, which were higher than those of the control group (1. 00 ± 0. 05) (all P < 0. 05). Compared with the model group, the expression levels of Caveolin-1 protein and p38 MAPK protein activity in the cartilage of the moxibustion group and blocker group decreased (both P < 0. 05). Compared with the blocker group, the expression levels of Caveolin-1 protein and p38 MAPK protein activity in the cartilage of moxibustion group increased (both P < 0. 05). Conclusions The expression levels of Caveolin-1 and the activity of p38 MAPK protein decrease in the cartilage of KOA rabbits after moxibustion treatment. Inhibition of abnormal activation of Caveolin-1/p38 MAPK signal pathway may be one of the molecular mechanisms of moxibustion. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of mild moxibustion on pathological morphology of skeletal muscle trigger point of myofascial pain rats as well as the influence on the changes of cyclooxygenase-2( COX-2),prostaglandin E2( PGE-2),interleukin-6( IL-6) and tumor necrosis factor-α( TNF-α). Methods Sixty-four SD rats were randomly divided into the control group( n = 24),model group( n = 24),and mild moxibustion group( n = 16). In the model group and moxibustion group,we adopted the method of beating combined with centrifugal movement to establish the myofascial pain trigger point models. The rats in the control group were fed normally. In the moxibustion group,after the end of the modeling,the rats began to receive moxibustion treatment for 7 days after routine feeding for 7 days. The control group and the model group were not treated with moxibustion. Eight rats were randomly selected from the three groups on the 1st day before moxibustion treatment and the 2nd and 14th days after moxibustion treatment. Tissues of myofascial trigger point or tissues in the same area were taken,stained with HE,and pathological morphology of tissues was observed under light microscope. The expression of COX-2,PGE2,IL-6,and TNF-α in the myofascial trigger point was detected by immunohistochemistry. Results In the control group,the muscle fibers in the myofascial trigger point were arranged regularly and uniformly,and the muscle spaces were consistent. No muscle fibers were twisted,broken,dissolved and necrotic,and with a little inflammatory cell infiltration. In the model group,the muscle fibers were disordered,broken and twisted at each stage. The myofascial trigger points were dissolved and necrotic before mild moxibustion treatment. At the end of mild moxibustion treatment,the muscle fibers were thickened,the size of the muscle gap was different,and there were a large number of inflammatory cells infiltrating. At the 14th day after mild moxibustion treatment,in the myofascial trigger point,partial fibers twisted to form nodules,and inflammatory cell infiltration was significantly reduced on the third day after treatment. The relative expression of COX-2,PGE2,IL-6,and TNF-α in each myofascial trigger point of the model group was significantly higher than that in the control group at the same time( all P < 0. 05),while COX-2,PGE2,IL-6,and TNF-α in the moxibustion group was significantly lower than that in the model group at the same time( all P < 0. 05),and the relative expression of COX-2,PGE2,IL-6,TNF-α in the myofascial trigger point on the 14th day after moxibustion treatment was significantly lower than that on the 3nd day after treatment( all P < 0. 05); no significant change was found in the expression of COX-2,PGE2,IL-6,and TNF-α in the myofascial trigger point of the model group and control group( all P> 0. 05). Conclusion Local mild moxibustion intervention can promote the repair of fibers of myofascial trigger point by reducing the recruitment of inflammatory cytokines in myofascial trigger point and then alleviating the inflammatory reaction. [ABSTRACT FROM AUTHOR]

目的观察艾灸治疗对帕金森病大鼠黑质磷酸化雷帕霉素靶蛋白(p-mtor)、磷酸化真核细胞起始因子4E结合蛋白1(p-4EBP1)及α-突触核蛋白(α-syn)表达的影响,探讨艾灸治疗帕金森病的作用机制。方法健康雄性SD大鼠60只,随机分为正常组、假手术组、帕金森病组、帕金森病艾灸组,每组15只。帕金森病组、帕金森病艾灸组采用颈背部皮下注射鱼藤酮法制备帕金森病模型,假手术组注射等浓度不含鱼藤酮的葵花油乳液,正常组不做处理。正常组、假手术组、帕金森病组不予艾灸;帕金森病艾灸组造模成功后选取足三里(双侧)、关元、风府穴施予艾灸治疗,1次/d,每次10 min,7 d为1个疗程,连续治疗2个疗程。然后对各组大鼠进行行为学评分;Western blotting检测各组大鼠黑质p-mtor、p-4EBP1,免疫组化法检测各组大鼠黑质α-syn。结果帕金森病组大鼠行为学评分与正常组和假手术组相比明显升高(P均<0.01);帕金森病艾灸组大鼠行为学评分较帕金森病组显著降低(P<0.01)。与正常组、假手术组相比,帕金森病组大鼠黑质p-mtor、p-4EBP1、α-syn表达量明显升高(P均<0.01);与帕金森病组相比,帕金森病艾灸组大鼠黑质p-mtor、p-4EBP1、α-syn表达量明显下降(P均<0.01)。结论艾灸足三里、关元、风府穴治疗帕金森病的作用机制可能是抑制了雷帕霉素靶蛋白/真核细胞起始因子4E结合蛋白通路的过度活化,遏止α-syn的过度表达,从而减轻过度表达的α-syn对多巴胺能神经元的毒性。 [ABSTRACT FROM AUTHOR]

目的　观察温针灸对类风湿关节炎（ＲＡ）大鼠抗血清抗环瓜氨酸（ＣＣＰ）抗体、类风湿因子（ＲＦ）的影响。方法　将５０只大鼠随机分为５组，每组１０只。Ａ～Ｄ组于左后足跖底部弗氏完全佐剂制作ＲＡ模型，造模成功后Ａ、Ｂ、Ｃ组分别予以温针灸、针刺、艾灸治疗，Ｄ组不予任何治疗；Ｅ组于左后足跖底部注射等量生理盐水，不做任何治疗。治疗３个疗程后，用ＥＬＩＳＡ法检测血清抗ＣＣＰ抗体、ＲＦ，ＨＥ染色观察跖趾关节病理。结果　与Ｅ组比较，Ｄ组血清抗ＣＣＰ抗体、ＲＦ水平均明显增高（Ｐ均＜０．０５）；与Ｄ组比较，Ａ～Ｃ组血清抗ＣＣＰ、ＲＦ水平降低（Ｐ均＜０．０５），Ａ组较Ｂ、Ｃ组降低明显（Ｐ均＜０．０５），Ｂ、Ｃ组无统计学差异。Ａ～Ｃ组跖趾关节滑膜炎症较Ｄ组减轻，Ａ组减轻最明显。结论　温针灸可降低ＲＡ大鼠血清抗ＣＣＰ抗体、ＲＦ水平，从而改善ＲＡ病理发挥治疗作用。 [ABSTRACT FROM AUTHOR]