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The rate and method of lime application modify soil chemical attributes, but the re-acidification process after liming remains poorly understood in the long-term. This study aimed to assess the long-term effect of surface and incorporated liming on shifting soil use from native grassland of South Brazilian Campos to no-tillage system (NTS) for grain production in a highly acidic sandy-loam Ultisol. In 1994, an experiment in a randomized block with split plot design and four replications was installed in Southern Brazil. The main plot consisted of lime application method (surface or incorporation by soil tillage), while the subplots were lime rates (0.0, 2.0, 8.5 and 17.0 Mg ha −1 ), corresponding, approximately, to 0, 1/8, 1/2, and 1 fold the recommended dose to rise soil pH to 6.0 in the 0–20 cm soil layer. The 2 Mg ha ‒1 treatment had reapplication every two years until soil accumulated 8 Mg ha ‒1 of lime. In October 2006 and 2012 (i.e., 12 and 18 years after lime application), soil samples were collected at every centimeter from 1 to 10 cm depth, every 2.5 cm from 10 to 25 cm, every 5 cm from 25 to 50 cm and 50 to 60 cm. The chemical attributes related to soil acidity were evaluated. Even after 18 years of lime application, the re-acidification process recovered only 50% of the exchangeable Al and 30% of the potential acidity compared to native soil. Lime incorporation before NTS adoption proved to be more rapid and efficient to mitigate soil acidity than surface liming, and it is suitable for correction of deep-layered soil acidity in the soil profile. Surface lime application induced a lower alkalinization front in the soil profile, and even after 18 years there was no neutralization of total exchangeable Al in the 10–20 cm layer. [ABSTRACT FROM AUTHOR]