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• BBU could harvest similar grain yield of rice with FP treatment. • N releasing rate of CRU was higher during late rice season than early rice season. • N release rate of S-CRU were slower and less sensitive to temperature than P-CRU. • S-BBU was more close to the N requirement of rice than P-BBU. Single basal application of bulk blending urea(BBU) which consists of both controlled-released urea(CRU) and conventional urea(U) has been proposed to facilitate fertilization and lower cost of CRU. Nevertheless, its effect on the growth, yield and N use efficiency(NUE) of rice in double rice cropping system under subtropical environment are still unknown. Field experiments were conducted at two different sites(Taishan county and Wengyuan county) simultaneously in Guangdong province to investigate the effects of single basal application of two types of BBU(P-BBU which consists of parabolic pattern CRU, and S-BBU which consists of sigmodial pattern CRU) on soil available N, grain yield and NUE. Conventional high-yield fertilization treatment with four split applications of U(FP) and no-N treatment(CK) were established as controls. The results demonstrated that BBU could provide similar or more adequate N nutrient for rice with FP during late rice season. BBU performed comparably to or better than FP on soil NH 4 +-N, dry matter, grain yield and NUE during late rice season. During early rice season, BBU performed comparably with FP on soil NH 4 +-N, N content of rice, dry matter, grain yield and NUE at Taishan site, while at Wengyuan site, it significantly reduced soil NH 4 +-N, N content of rice, dry matter and NUE compared with FP, and reduced grain yield slightly. Release tests in water and paddy field were conducted in order to evaluate the releasing dynamics of parabolic and sigmodial pattern CRU. Results showed that the N release rate of CRU in paddy field during early rice season was significantly slower than that during late rice season. This was closely correlated with the changing pattern of accumulated temperature. The N release rate of parabolic CRU in paddy field was faster and more sensitive to changing temperature than sigmodial CRU. Non-linear regression analysis shows that the N release patterns of sigmodial CRU in paddy fields were more close to N uptake of rice during both rice seasons. The grain yield of rice and NUE in S-BBU was higher slightly than P-BBU during both rice seasons. These results demonstrated that BBU obtained similar grain yield in double rice cropping system in South China, and S-BBU treatment is better for N requirement of rice during both seasons. Future studies should focus on optimizing and customizing the blending ratio of BBU for each rice season in double rice cropping system to increase crop yield and the robustness of BBU to changing weather conditions. [ABSTRACT FROM AUTHOR]