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[Objective] The integrity of chloroplast structure and the normal expression of key genes in photosynthesis play an important role in photosynthesis. [Method]This paper researched the influence of the volatile oil of Chenopodium ambrosioides L., and its two main components (α-terpinene and cvmene) on chloroplast ultrastructure and 4 key photosynthetic genes in leaves of Vicia faba seedlings by the technology of TEM and RT-PCR, and in order to intensively study the allelopathic mechanism of C. ambrosioides. [Result]The results showed that the volatile oil, α-terpinene and cymene of C. ambrosioides damaged the ultrastructure of chloroplast in V. faba leaves, and the chloroplast was disorganized with tumid and damaged membrane structure, heterogeneous substrate, disorganized granum-thvlakoid, disappeared starch grain and twisty incompact lamella scattered from the wall to the center of the cell. The relative expression of Ribulose carboxylase gene (Rbes-3C), optical system II CP47 protein gene(PsbB), ATP synthetase beta subunit gene(beta) and plasmid blue pigment genes(PetE) were changed in different extent during photosynthesis. The volatile oil of C. ambrosioides increased the relative expression of these 4 genes. Cymene decreased the relative expression of Rbcs-3C and PsbB, and increased the relative expression of lieta and PetE. The α-terpinene decreased the relative expression of PsbB and increased others. The allelopathic synthesis effect of three treatments on these gene expressions from most significant to least significant were volatile oil, α-terpinene and cymene. [Conclusion] These results indicated that the damage of chloroplast ultrastructure and interference of photosynthetic key gene expression were one part of the allelopalhic mechanism of C. ambrasioides and different components of the volatile oil from C. ambrosioides had a synergistic effect in allelopathy. [ABSTRACT FROM AUTHOR]

【Objective】In order to explore the role of lipid in buffalo oocytes in vitro maturation process,and optimize the oocytes mature system,the lipid content and distribution of buffalo mature stage GV immature and MII oocytes were investigated.【Method】The ovaries of buffalo were collected from a slaughterhouse in Nanning city to collect the cumulus oocyte complexes,one part of the complexes were used to collect immature oocytes in GV stage,the MII mature oocytes were obtained from another part after in vitro maturation for 22-24 hours. After that,the oocytes of GV and MII phases were placed into 10 μg/m L Nile red stain solution. After 4-5 hours of staining in a dark environment at room temperature,oocytes were observed under a fluorescence microscope. According to the obtained photos,the lipid distribution pattern in oocytes was observed,and the fluorescence intensity was calculated with the analysis of Image J software,and the lipid content in oocytes was calculated with the fluorescence intensity.【Resul】Lipid content of MII stage was significantly lower than that in GV stage(66.63 ± 1. 197 vs. 70. 81 ± 1. 328,P < 0. 05). There was no significant difference in lipid distribution patterns in oocytes of GV stage and MII stage(peripheral: 3. 148 ± 2. 298 vs. 5. 342 ± 3. 858; semi-peripheral: 30. 85 ± 4. 042 vs. 35. 70 ± 4. 265; 66. 00 ± 3. 257 vs. 60. 60 ±3. 933,P > 0. 05).【Conclusion】During the maturation process of the water buffalo oocytes from immature GV stage to mature MII stage,the lipid content is significantly reduced,and the lipid distribution pattern is not changed before and after the vitro maturation of the oocytes. [ABSTRACT FROM AUTHOR]