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Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This study optimized the four factors (template DNA, primers, Mix mixture dosage and annealing temperature) that affect the target start codon polymorphism of oatmeal polymerase chain reaction (SCoT-PCR), and passed The optimal system has been screened for polymorphic primers. [Method] Using 4 copies of oat variety DNA as template, L9 (3³ ) orthogonal test was used to determine the optimal reaction system for the amount of template DNA, primers, and Mix mixture, and 8 gradients were set in the temperature range of 48～54℃. Annealing temperature screening, and polymorphism screening and verification of 80 primers through the optimal system. [Results] The total volume of the optimized reaction system was 20μl, containing 2μl template DNA (10 ng·μl -1 ), 2μl primers (10μmol·L -1 ), 9μl Mix and 7μl ddH2O. 36 primers with good amplification effect and polymorphism and their optimal annealing temperature were screened out. [Conclusion] The oat SCoT-PCR amplification system was successfully optimized and established. The establishment of this system will provide an important reference for the genetic diversity analysis of oat varieties, the construction of variety fingerprints, and molecular marker-assisted breeding. [ABSTRACT FROM AUTHOR]

Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The leaves of Chirita tribracteata W. T. Wan/ were used as material，and the disinfectant method，callus induction，callus differentiation， proliferation subculture，rootin/ culture and vitrification of plant were studied. The results showed that the disinfectant method was 75 % ethanol for 10s with 0 .1 % H/Cl^for 12 - 16 min. The suitable medium of callus inducin/ and differentiation from leaves was MS + 6- BA 2. 0 m /L + NAA 0. 1 m / L ，the suitable medium of proliferation culture was MS + 6-BA 1. 0 m /L + IBA 0 .1 - 0 . 5 m /L or MS + 6- BA 1. 0 m /L + NAA 0 .1 - 0 . 5 m / L ; the optimum medium for rootin/ was 1/2MS + IBA 0. 1 - 0 . 3 m / L ，rootin/ rate was 100. 00 % . The vitrification of the plant could be reduced by addin/ 6-BA or addin/ low concentration 6-BA or addin/ the appropriate amount of activated carbon in the medium. [ABSTRACT FROM AUTHOR]

Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Rice blast is one of the most serious disease of rice. The induced expressions of key enzyme genes of plant signaling pathway and four pathogenesis-related protein genes were analyzed in rice leaves by using three rice varieties (LTH, J6 and J27) and one special blast isolate (J32-6). The results showed that Os LOX, Os AOS and Os PAL were induced by isolate compatible and incompatible interactions. Jasmonate and salicylate dependent defense signaling pathways were activated. Os PR1 a, Os PR1 b, Os PR3-1 and Os PR4 were expressed for longer time in the leaves of incompatible rice plants, so J6 and J27 exhibited disease resistance. While most of Os PR were expressed for lower, short time or non expression in the leaves of compatible rice plants, so LTH was susceptible to rice blast. [ABSTRACT FROM AUTHOR]

Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)