1. Use of endometrial cytology and metabolic profiles for selection of embryo donor cows
- Author
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F. Ismael Fernandez-Sanchez, Monica Barrio-Lopez, Luis A. Quintela-Arias, Juan J. Becerra-Gonzalez, Ana I. Peña-Martinez, Daniel Martinez-Bello, Pedro J. Garcia-Herradon, and Carlos C. Perez-Marín
- Subjects
dairy cattle ,embryo transfer ,serum chemistry parameters ,endometritis ,neutrophils ,Agriculture - Abstract
The aim of this study was to evaluate the use of endometrial cytology and metabolic profiles for selection of donor cows in embryo transfer programmes. For this purpose, 69 clinically healthy Holstein cows were enrolled in the study. At the start of the superovulation procedure (Day 0), blood and endometrial samples were obtained to determine metabolic and uterine status, respectively. The cows were then subjected to porcine follicle stimulating hormone (pFSH) superovulation treatment, and embryos were recovered after 7 days. The mean number of embryos obtained per flush was 9.89±8.21 (4.63±5.34 viable embryos, 0.82±2.01 degenerated embryos and 4.57±6.44 unfertilized ova). The following statistically significant variables were entered in a regression model: beta-hydroxybutyrate, serum cholesterol, body condition, number of calvings and percentage of neutrophils. In almost all cases, the model explained some percentage of the variance: total number of embryos, 4.8% (pp=0.051); and number of unfertilized ova, 14.2% (ppp
- Published
- 2014
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