1. Lysosome-targeted two-photon fluorescent probe for detection of hypobromous acid in vitro and in vivo.
- Author
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Ma, Chen, Ma, Minrui, Zhang, Yida, Zhu, Xinyue, Zhou, Lin, Fang, Ran, Liu, Xiaoyan, and Zhang, Haixia
- Subjects
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LYSOSOMES , *COLLAGEN , *NAPHTHALIMIDES , *FLUORESCENCE , *HELA cells - Abstract
Abstract It is found that hypobromous acid (HOBr) can affect the activity of type IV collagen. Herein, we synthesized a lysosome-targeted fluorescence probe NA-lyso based on Suzuki coupling reaction with naphthalimide as a fluorescent group. HOBr can oxidize the amino group and methylthio group, which increased the degree of conjugation of the probe, thereby affecting its optical properties. Accordingly, it can establish a method for the specific detection of HOBr. NA-lyso has the properties including fast response, high fluorescence quantum yield (Φ = 59.17%), high selectivity, low cytotoxicity and good membrane-permeability. The probe can locate to lysosome of cells. The potential of the probe as biosensor for HOBr was demonstrated by imaging of exogenous and endogenous HOBr in living cells and in mice. In consequence, NA-lyso is expected to be a powerful tool to detect HOBr in complex biosystem and provides a means of exploring physiological functions associated with HOBr in living organisms. Graphical Abstract Unlabelled Image Highlights • A lysosome-targeted fluorescence probe NA-lyso for monitoring HOBr was synthesized. • The probe can respond to HOBr with outstanding sensitivity and selectivity. • The probe can target in lysosome and monitoring the level of HOBr in Hela cells. • The probe can also be used to image HOBr in vivo. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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