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[Display omitted] • A novel spectral fusion diagnosis technology is proposed. • A new and efficient DBRAN fusion network is designed to process spectral data. • DBRAN fusion technology has achieved 100% accuracy, sensitivity and specificity in SLE disease classification, achieving significant performance improvement compared to single-modality (ORS or SERS) methods. As artificial intelligence technology gains widespread adoption in biomedicine, the exploration of integrating biofluidic Raman spectroscopy for enhanced disease diagnosis opens up new prospects for the practical application of Raman spectroscopy in clinical settings. However, for systemic lupus erythematosus (SLE), origin Raman spectral data (ORS) have relatively weak signals, making it challenging to obtain ideal classification results. Although the surface enhancement technique can enhance the scattering signal of Raman spectroscopic data, the sensitivity of the SERS substrate to airborne impurities and the inhomogeneous distribution of hotspots degrade part of the signal. To fully utilize both kinds of data, this paper proposes a two-branch residual-attention network (DBRAN) fusion technique, which allows the ORS to complement the degraded portion and thus improve the model's classification accuracy. The features are extracted using the residual module, which retains the original features while extracting the deep features. At the same time, the study incorporates the attention module in both the upper and lower branches to handle the weight allocation of the two modal features more efficiently. The experimental results demonstrate that both the low-level fusion method and the intermediate-level fusion method can significantly improve the diagnostic accuracy of SLE disease classification compared with a single modality, in which the intermediate-level fusion of DBRAN achieves 100% classification accuracy, sensitivity, and specificity. The accuracy is improved by 10% and 7% compared with the ORS unimodal and the SERS unimodal modalities, respectively. The experiment, by fusing the multimodal spectral, realized rapid diagnosis of SLE disease by fusing multimodal spectral data, which provides a reference idea in the field of Raman spectroscopy and can be further promoted to clinical practical applications in the future. [ABSTRACT FROM AUTHOR]

After collecting Raman spectral data of 12 compounds from commonly used Uyghur medicinal materials, the mixture data are synthesized using computer simulation methods. An accurate identification method for Uyghur medicine components based on Raman spectroscopy and multi-label deep learning is constructed, and the performance of the model Mix2Com is evaluated using several metrics such as accuracy, precision, recall value, and F1 score. [Display omitted] • Existing methods identifying Uyghur medicinal components aren't fit for large samples. • A new method based on Raman spectroscopy and multi-label learning is proposed. • Macro-parallel identification of components is achieved. • The model Mix2Com is constructed with LSTM and Attention introduced. • Mix2Com achieves 90.76% accuracy, better than XGBoost and DeepRaman. Uyghur medicine is one of the four major ethnic medicines in China and is a component of traditional Chinese medicine. The intrinsic quality of Uyghur medicinal materials will directly affect the clinical efficacy of Uyghur medicinal preparations. However, in recent years, problems such as adulteration of Uyghur medicinal materials and foreign bodies with the same name still exist, so it is necessary to strengthen the quality control of Uyghur medicines to guarantee Uyghur medicinal efficacy. Identifying the components of Uyghur medicines can clarify the types of medicinal materials used, is a crucial step to realizing the quality control of Uyghur medicines, and is also an important step in screening the effective components of Uyghur medicines. Currently, the method of identifying the components of Uyghur medicines relies on manual detection, which has the problems of high toxicity of the unfolding agent, poor stability, high cost, low efficiency, etc. Therefore, this paper proposes a method based on Raman spectroscopy and multi-label deep learning model to construct a model Mix2Com for accurate identification of Uyghur medicine components. The experiments use computer-simulated mixtures as the dataset, introduce the Long Short-Term Memory Model (LSTM) and Attention mechanism to encode the Raman spectral data, use multiple parallel networks for decoding, and ultimately realize the macro parallel prediction of medicine components. The results show that the model is trained to achieve 90.76% accuracy, 99.41% precision, 95.42% recall value and 97.37% F1 score. Compared to the traditional XGBoost model, the method proposed in the experiment improves the accuracy by 49% and the recall value by 18%; compared with the DeepRaman model, the accuracy is improved by 9% and the recall value is improved by 14%. The method proposed in this paper provides a new solution for the accurate identification of Uyghur medicinal components. It helps to improve the quality standard of Uyghur medicinal materials, advance the research on screening of effective chemical components of Uyghur medicines and their action mechanisms, and then promote the modernization and development of Uyghur medicine. [ABSTRACT FROM AUTHOR]

[Display omitted] • Sensitive and reproducible CuNPs@AgNPs@PSB SERS substrates were prepared using a self-assembly approach. • The CuNPs@AgNPs structure was successfully synthesized on the Porous silicon Bragg reflector (PSB) surface using the reduction of PSB and copper nanoparticles. • Low concentration detection of R6G and CPPU was completed. • The prepared high-performance and high-sensitivity SERS substrate have a very promising application in detection technology. The development of a highly sensitive, synthetically simple and economical SERS substrate is technically very important. A fast, economical, sensitive and reproducible CuNPs@AgNPs@ Porous silicon Bragg reflector (PSB) SERS substrate was prepared by electrochemical etching and in situ reduction method. The developed CuNPs@AgNPs@PSB has a large specific surface area and abundant "hot spot" region, which makes the SERS performance excellent. Meanwhile, the successful synthesis of CuNPs@AgNPs can not only modulate the plasmon resonance properties of nanoparticles, but also effectively prolong the time stability of Cu nanoparticles. The basic performance of the substrate was evaluated using rhodamine 6G (R6G). (Detection limit reached 10−15 M, R2 = 0.9882, RSD = 5.3 %) The detection limit of Forchlorfenuron was 10 μg/L. The standard curve with a regression coefficient of 0.979 was established in the low concentration range of 10 μg/L −100 μg/L. This indicates that the prepared substrates can accomplish the detection of pesticide residues in the low concentration range. The prepared high-performance and high-sensitivity SERS substrate have a very promising application in detection technology. [ABSTRACT FROM AUTHOR]

[Display omitted] • Rapid diagnosis of systemic lupus erythematosus achieved using Raman spectroscopy. • Achieving accurate discrimination between SLE and SS. • Established three models (ANN, ResNet, SNN) and compared their classification effect. Multiple organs are affected by the autoimmune inflammatory connective tissue disease known as systemic lupus erythematosus (SLE). If not diagnosed and treated in a timely manner, it can lead to nephritis and damage to the blood system in severe cases, resulting in the patient's death. Therefore, correct and timely diagnosis and treatment are essential for patients. In this study, a framework based on neural network algorithm and Raman spectroscopy technique was established to diagnose SLE patients. Firstly, we pre-processed the obtained Raman data by three methods: baseline correction, smoothing processing and normalization methods, before using it as input for the model, and then ANN, ResNet and SNN classification models were established. The respective classification accuracies for SLE patients were 89.61%, 85.71%, and 95.65% for the three models, with corresponding AUC values of 0.8772, 0.8100, and 0.9555. The results of the experimental indicate that SNN possesses a good classification effect, and the number of model parameters is only 525,826, which is 414,221 less than that of ResNet model. Since the network only uses 0 and 1 to transmit information, and only has basic operations such as summation, compared with the second-generation artificial neural network, which simplifies the product operation of floating point numbers into multiple addition operations, the network has low energy consumption and is suitable for embedding portable Raman spectrometer for clinical diagnosis. This research highlights the significant potential for quick and precise SLE patient discrimination offered by Raman spectroscopy in conjunction with spiking neural networks. [ABSTRACT FROM AUTHOR]

[Display omitted] • Nanoparticles are uniformly embedded in PSi chips with 40–60 nm pore size, enhancing BRC serum signal. • The Ag 2 O-Ag core–shell structure effectively prevents silver from being oxidized by sulfur, giving the chip a shelf life of six months. • The PCA-t-SNE rapid screening model combined with the SERS microarray provides the basis for the development of a low-cost, rapid and sensitive technique for early screening of breast cancer. Ag 2 O-Ag-PSi (porous silicon) surface-enhanced Raman scattering (SERS) chip was successfully synthesized by electrochemical corrosion, in situ reduction and heat treatment technology. The influence of different heat treatment temperature on SERS performance of the chip is studied. The results show that the chip treated at 300 °C has the best SERS performance. The chip was composed of Ag 2 O-Ag nano core shell with a diameter of 40–60 nm and porous silicon substrate. Then, the optimized chip was used to perform SERS test on serum samples from 30 healthy volunteers and 30 early breast cancer patients, and the baseline was corrected by LabSpec6 software. Finally, the data were analyzed by principal component analysis combined with t-distributed Stochastic Neighbor Embedding (PCA-t-SNE). The results showed that the accuracy of the improved substrate combined with multivariate statistical method was 98%. The shelf life of the chips exceeded six months due to the presence of the Ag 2 O shell. This study provides a basis for developing a low-cost rapid and sensitive early screening technology for breast cancer. [ABSTRACT FROM AUTHOR]

[Display omitted] • Differences in the biochemical composition of five types of cervical tissue were analyzed. • A multi-layered attention mechanism feature fusion architecture (MAFA) is proposed. • MAFA can effectively improve the classification accuracy of deep learning models for tissue samples. • MAFA as a model building method can be easily combined with a variety of deep learning models. Cervical cancer ranks among the most prevalent forms of gynecological malignancies. Timely identification of cervical lesions and prompt intervention can effectively prevent the development of cervical cancer or enhance patients' chances of survival. In this study, we propose an innovative method based on Raman spectroscopy, i.e., a multi-level SENet attention mechanism feature fusion architecture (MAFA) for rapid diagnosis of cervical cancer and precancerous lesions. The convolution process of this architecture can extract features from shallow to deep layers, and the attention mechanism is added to achieve the fusion of features from different layers. The added attention mechanism can automatically determine the importance of each layer feature channel and assign weight values to that layer according to the importance of each layer to achieve the purpose of focusing the model on certain waveform features and improve the targeting of model learning. We collected Raman spectra of 212 cervical tissues containing cervical cancer and its precancerous lesions.The experimental results show that MAFA can effectively improve the diagnostic accuracy of VGGNet, GoogLeNet and ResNet models in the validation of Raman spectral data of cervical tissue. Among them, ResNet performed the best, with the highest average accuracy, precision, recall and F1-Score of 82.36%, 84.00%, 82.35% and 82.26%, respectively, when no feature fusion was performed. The evaluation metrics improved by 4.91%, 3.97%, 4.97%, and 5.06%, respectively, after using the MAFA; they also improved by 4.16%, 2.90%, 4.17%, and 4.32%, respectively, compared with the model that directly performs feature fusion without using the attention mechanism. Therefore, the MAFA proposed in this study is better than that of the neural network that directly fuses the features of each convolutional layer. The experimental results show that the performance of the MAFA proposed in this paper is significantly higher than that of traditional deep learning algorithms, indicating that the present architecture can effectively improve the diagnostic accuracy of deep learning networks for cervical cancer. [ABSTRACT FROM AUTHOR]

Abstract This study presents a rapid and non-invasive method to screen high renin hypertension using serum Raman spectroscopy combined with different classification algorithms. The serum samples taken from 24 high renin hypertension patients and 22 non-high renin hypertension samples were measured in this experiment. Tentative assignments of the Raman peaks in the measured serum spectra suggested specific biomolecular changes between the groups. Principal component analysis (PCA) was first used for feature extraction and reduced the dimension of high-dimension spectral data. Then, support vector machine (SVM), linear discriminant analysis (LDA) and k-nearest neighbor (KNN) algorithms were employed to establish the discriminant diagnostic models. The accuracies of 93.5%, 93.5% and 89.1% were obtained from PCA-SVM, PCA-LDA and PCA-KNN models, respectively. The results from our study demonstrate that the serum Raman spectroscopy technique combined with multivariate statistical methods have great potential for the screening of high renin hypertension. This technique could be used to develop a portable, rapid, and non-invasive device for screening high renin hypertension. Graphical abstract Raman spectroscopy technique combined with multivariate statistical methods for the screening of high renin hypertension. Unlabelled Image Highlights • Raman spectroscopy has the potential to screen high renin hypertension patients. • The high accuracy of 93.5% is obtained from multivariate statistical method. • Our method is expected to develop a clinical diagnostic tool. [ABSTRACT FROM AUTHOR]

[Display omitted] • For the first time, Raman spectroscopy combined with deep learning is used for the classification and identification of red wine origin. • Corresponding biomarkers identified by Raman spectroscopy of red wine. • Classification of red wine origin by PCA analysis. • Comparative experiment of four deep learning models. • The combination of MCNN and Raman spectroscopy achieves the best results for the classification of red wine origin. In this study, we combined Raman spectroscopy with deep learning for the first time to establish an accurate, simple, and fast method to identify the origin of red wines. We collected Raman spectra from 200 red wine samples of the Cabernet Sauvignon variety from four different origins with a portable Raman spectrometer. The red wine samples, made in 2021, were from the same producer in China. Differences were found by analyzing the Raman spectra of red wine samples. These differences are mainly caused by ethanol, carboxylic acids, and polyphenols. After further analysis, for different origins, the different performances of these substances on the Raman spectrum are related to the climate and geographical conditions of the origin. The Raman spectra were analyzed by principal component analysis (PCA). The data with PCA dimensionality reduction were imported into an artificial neural network (ANN), multifeature fusion convolutional neural network (MCNN), GoogLeNet, and residual neural network (ResNet) to establish red wine origin identification models. The classification results of the model prove that climate, geography, and other conditions can provide support for the classification of red wine origin. The experiments showed that all four models performed well, among which MCNN performed the best with 93.2% classification accuracy, and the area under the curve (AUC) was 0.987. This study provides a new means to classify the origin of red wine and opens up new ideas for identifying origins in the food field. [ABSTRACT FROM AUTHOR]

[Display omitted] • Differences in the biochemical composition of seven types of tissues are analyzed. • A new one-dimensional convolutional hierarchical network (H-CNN) is proposed. • H-CNN classifies tissue samples in a hierarchy from coarse to fine. • H-CNN outperforms traditional classification models. Cervical cancer is one of the most common cancers with a long latent period and slow onset process. Early and accurate identification of the stage of cervical cancer can significantly improve the cure rate and patient survival time. In this study, we collected 699 Raman spectral data of tissue sections from 233 different patients. We analyzed and compared the characteristics and differences of the mean Raman spectra of the seven tissues and pointed out the main differences in the biochemical composition of the seven tissues. In this study, 1D hierarchical convolutional neural network (H-CNN) is proposed by integrating the prior knowledge of hierarchical classification relations with the research of deep learning in Raman spectroscopy. H-CNN is based on CNN and is added with three network branches. Hierarchical classification is performed from coarse to fine for tissue samples of cervicitis, Low-grade Squamous Cell Carcinoma, High-grade Squamous Cell Carcinoma, Well Differentiated Squamous Cell Carcinoma, Moderately Differentiated Squamous Cell Carcinoma, Poorly Differentiated Squamous Cell Carcinoma and cervical adenocarcinoma. To evaluate the recognition performance of H-CNN, we compared it with traditional methods such as Bayesian classifier (NB), decision tree classifier (DT), support vector machine classifier (SVM) and CNN. The experimental results show that H-CNN can accurately identify different classes of tissue sections and has apparent advantages in several aspects such as recognition accuracy, stability and sensitivity compared with the other four traditional recognition methods. The classification Macro-Accuracy of H-CNN can reach 94.91%, Macro-Recall can reach 95.31%, Macro-F1 can reach 95.23%, and Macro-AUC can reach 97.35%. The hierarchical classification method proposed in this study can diagnose patients more accurately. This could lay the foundation for further research on Raman spectroscopy as a clinical diagnostic method for cervical cancer. [ABSTRACT FROM AUTHOR]

[Display omitted] • Triple-negative breast cancer and HER2-positive breast cancer are highly malignant, and early diagnosis is helpful to improve the chances of cure. • Serum Raman spectroscopy combined with deep learning algorithms to diagnose triple-negative breast cancer and HER2-positive breast cancer and achieved great diagnostic results. • The proposed method is helpful for early breast cancer diagnosis and can provide a reference for breast cancer patients to make a personalised diagnosis. Breast cancer is common in women, and its number of patients ranks first among female malignant tumors. Breast cancer is highly heterogeneous, and different types of breast cancer have different biological behaviors and prognoses. Therefore, identifying the different types of breast cancer is of great help in formulating individualized treatment plans. Based on serum Raman spectroscopy and deep learning algorithms, we propose a fast and low-cost diagnosis method for screening triple-negative breast cancer, human epidermal growth factor receptor 2 (HER2)-positive breast cancer, and healthy controls. We collected 75 serum samples in this study, including 23 triple-negative breast cancers, 22 HER2-positive breast cancers, and 30 healthy controls. Using the preprocessed Raman spectra as the input of deep learning, three deep learning models, neural network language model (NNLM), bidirectional long-short-term memory network (BiLSTM), and convolutional neural network (CNN), were established, and the accuracy rates of the three models were 87.78%, 90.37%, and 91.11%, respectively. The experimental results demonstrate the feasibility of serum Raman spectroscopy combined with deep learning algorithms to diagnose breast cancer, which can be used as an effective auxiliary diagnosis method for breast cancer. [ABSTRACT FROM AUTHOR]

[Display omitted] • A novel spectral fusion diagnosis technology is proposed. • A new and efficient MFCNN network is designed to process spectral data. • This study explored the potential of spectral fusion technology for the diagnosis of multiple cancers in this complex clinical environment. According to the limited molecular information reflected by single spectroscopy, and the complementarity of FTIR spectroscopy and Raman spectroscopy, we propose a novel diagnostic technology combining multispectral fusion and deep learning. We used serum samples from 45 healthy controls, 44 non-small cell lung cancer (NSCLC), 38 glioma and 37 esophageal cancer patients, and the Raman spectra and FTIR spectra were collected respectively. Then we performed low-level fusion and feature fusion on the spectral, and used SVM, Convolutional Neural Network-Long-Short Term Memory (CNN-LSTM) and the multi-scale convolutional fusion neural network (MFCNN). The accuracy of low-level fusion and feature fusion models are improved by about 10% compared with single spectral models. [ABSTRACT FROM AUTHOR]

This research innovatively combines Fourier transform infrared spectra, Raman spectra and its first-derivative spectra to develop a rapid diagnosis method for renal cell carcinoma (RCC). The spectral features are extracted by principal component analysis (PCA), and then the normalized fused data are used as input to the improved AlexNet model and MCNN model to train the models. Among them, the AlexNet model performed better, with a classification accuracy rate of 93%. [Display omitted] • It is proposed for the first time to combine FT-IR, Raman spectra and their first-derivative spectra to develop a rapid diagnosis method for renal cell carcinoma. • The accuracy of the multi-spectral fusion method (93%) is greatly improved compared with that of single infrared spectroscopy (74%), signal Raman spectroscopy (75%) and the fusion of infrared spectroscopy and Raman spectroscopy (79%). • It shows that multi-spectral fusion technology combined with deep learning algorithms has great potential in the diagnosis of RCC. This research innovatively combines FT-IR, Raman spectroscopy and their first-derivative spectroscopy to develop a rapid diagnosis method for renal cell carcinoma (RCC). After measuring the Raman spectra and FT-IR spectra of 45 cases of control subjects and 28 cases of RCC, the first derivative of the infrared spectra and the Raman spectra were calculated respectively. Principal component analysis (PCA) was used to extract the features of the infrared spectra, first-derivative infrared spectra, Raman spectra and first-derivative Raman spectra. Then the four feature matrices were merged as fused spectral feature matrices. The fused matrices were used as the input of AlexNet and MCNN. The fused spectral feature matrices were used as the input of AlexNet and MCNN. The adjusted AlexNet model performed better, and the classification accuracy of the fused spectral data is 93%. Compared with the classification results of infrared spectra (74%), Raman spectra (75%) and the fusion of infrared and Raman spectra (79%) combined with the adjusted AlexNet model, the classification result of the fusion of infrared spectra, Raman spectra and their first-derivative was significantly improved. The experimental results show that infrared spectroscopy, Raman spectroscopy and their first-derivative fusion technology combined with deep learning algorithms has great potential in the diagnosis of RCC. [ABSTRACT FROM AUTHOR]

[Display omitted] • Innovative use of generative technology established the recognition models of cumin and fennel from three regions. • This study also established the models for cumin and fennel in the same region. • This study provides a new method for classification of NIR spectroscopy. Cumin (Cuminum cyminum) and fennel (Foeniculum vulgare) are widely used seasonings and play a very important role in industries such as breeding, cosmetics, winemaking, drug discovery, and nano-synthetic materials. However, studies have shown that cumin and fennel from different regions not only differ greatly in the content of lipids, phenols and proteins but also the substances contained in their essential oils are also different. Therefore, realizing precise identification of cumin and fennel from different regions will greatly help in quality control, market fraud and production industrialization. In this experiment, cumin and fennel samples were collected from each region, a total of 480 NIR spectra were collected. We used deep learning and traditional machine learning algorithms combined with near infrared (NIR) spectroscopy to identify their origin. To obtain the model with the best generalization performance and classification accuracy, we used principal component analysis (PCA) to reduce spectral data dimensionality after Rubberband baseline correction, and then established classification models including quadratic discriminant analysis based on PCA (PCA-QDA) and multilayer perceptron based on PCA (PCA-MLP). We also directly input the spectral data after baseline correction into convolutional neural networks (CNN) and generative adversarial networks (GAN). The experimental results show that GAN is more accurate than the PCA-QDA, PCA-MLP and CNN models, and the classification accuracy reached 100%. In the cumin and fennel classification experiment in the same region, the four models achieve great classification results from three regions under the condition that all model parameters remain unchanged. The experimental results show that when the training data are limited and the dimension is high, the model obtained by GAN using competitive learning has more generalization ability and higher classification accuracy. It also provides a new method for solving the problem of limited training data in food research and medical diagnosis in the future. [ABSTRACT FROM AUTHOR]

In this research, PLSR, GA-SVR and ELM models combined with sheep serum Raman spectroscopy were used for quantitative detection, the results of PLSR determination are better (a). In order to further improve the performance of the model, the SPA is used for band selection (c). The optimized quantitative model result (b) of DOSC-SPA-PLSR-MN performs well. • It is proposed for the first time to use sheep serum Raman spectroscopy combined with a quantitative model to determine the concentration (/enzyme activity) of seven indicators. • It is the first time to deviation standardization of the model evaluation index for many kinds of substances. • The comparison results of SPA band selection show that there is a correlation among the indicators in the serum. • The optimized DOSC-SPA-PLSR-DS quantitative model shows that the average R p 2 of the seven indicators is 0.9834. Hepatic fascioliasis, ketosis of pregnancy, toxemia of pregnancy and other common sheep diseases will directly affect the concentration (/enzymatic activity) of seven indicators, such as cortisol and high-density lipoprotein cholesterol (HDL-C) in sheep serum. Whether the concentrations (/enzymatic activity) of these indicators can be detected quickly will directly affect the prevention of sheep diseases and the targeted adjustment of breeding methods, thereby affecting the economic benefits of sheep breeding. In this research, we established partial least square regression (PLSR), support vector regression based on genetic algorithm optimization (GA-SVR) and extreme learning machine (ELM) models. Due to the large differences in the content of different substances, it is difficult to directly use the RMSE to evaluate the quantitative effect of the model. This study is the first to propose conducting deviation standardization (DS) for the determination results of various substances. To further improve the performance of the model, we use the successive projections algorithm (SPA) to optimize feature extraction and combine it with the better-performing PLSR model for training. The results show that the optimized DOSC-SPA-PLSR-DS quantitative model has better determination results for 101 sheep serum samples. The average RMSE p* of the concentration of the six substances decreased from 0.0408 to 0.0387, the R p 2 increased from 0.9758 to 0.9846, and the running time was reduced from 0.1659 to 0.0008 s. And the determination performance of lipase (LPS) enzymatic activity has also been improved. The results of this research show that sheep serum Raman spectroscopy combined with DOSC-SPA-PLSR-DS optimization can efficiently monitor the concentration (/enzyme activity) of seven indicators in real time and provide a new strategy for future intelligent supervision of animal husbandry. [ABSTRACT FROM AUTHOR]

Echinococcosis is a zoonotic parasitic disease transmitted by animals and distributed all over the world. There is no standardized and widely accepted treatment method, and early and accurate diagnosis is crucial for the prevention and cure of echinococcosis. Here, we explored the feasibility of using derivative Raman in combination with autofluorescence (AF) to improve the diagnosis performance of echinococcosis. The spectra of serum samples from patients with echinococcosis, as well as healthy volunteers, were recorded at 633 nm excitation. The normalized mean Raman spectra showed that there is a decrease in the relative amounts of β carotene and phenylalanine and an increase in the percentage of tryptophan, tyrosine, and glutamic acid contents in the serum of echinococcosis patients as compared to that of healthy subjects. Then, principal components analysis (PCA), combined with linear discriminant analysis (LDA), were adopted to distinguish echinococcosis patients from healthy volunteers. Based on the area under the ROC curve (AUC) value, the derivative Raman + AF spectral data set achieved the optimal results. The AUC value was improved by 0.08 for derivative Raman + AF (AUC = 0.98), compared to Raman alone. The results demonstrated that the fusion of derivative Raman and AF could effectively improve the performance of the diagnostic model, and this technique has great application potential in the clinical screening of echinococcosis. Combining derivative Raman with autofluorescence can improve the performance of echinococcosis diagnosis model. Unlabelled Image • Early and accurate diagnosis is the key to prevent and treat echinococcosis. • The autofluorescence background contains useful information for diagnosis. • The optimal result was obtained by combining derivative Raman with autofluorescence. • The proposed methods could possibly be used to diagnose other diseases. [ABSTRACT FROM AUTHOR]