1. Development of a water-soluble near-infrared fluorescent probe for endogenous cysteine imaging.
- Author
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Li, Yiling, He, Xu, Huang, Yibing, Xu, Longbin, Zhao, Lihe, Li, Xiaolei, Sun, Ying, Wang, Xinghua, Ma, Pinyi, and Song, Daqian
- Subjects
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CYSTEINE , *FLUORESCENT probes , *NUCLEOPHILIC substitution reactions , *DENSITY functional theory , *DETECTION limit - Abstract
We designed and synthesized a water-soluble near-infrared (NIR) fluorescent probe with the recognition unit of the cyanine-like structure and acrylate group. Through an aromatic ring nucleophilic substitution reaction based on sulfhydryl moiety, an off-on fluorescence response toward cysteine (Cys) was realized. The probe exhibited excellent spectral performance with an emission wavelength of 720 nm and a detection limit of 0.20 μM. The spectral properties, selectivity and anti-interference performance of the probe were systematically investigated. Density functional theory (DFT) calculations were conducted to clarify the luminescence mechanism of the probe. Furthermore, the probe was successfully applied to the detection of free Cys in human serum and the NIR imaging of endogenous Cys in living cells. Thus, the probe has a promising application prospect in clinical diagnosis and fluorescence imaging. Image 1 • Development of a novel near-infrared fluorophore (MTR). • Design and application of fluorescent probe (MTR-C) for the detection of endogenous Cys. • Successfully verified the experimental results by the theoretical calculation study. • Successfully tracked the fluctuations of Cys levels in BEL-7402 cells. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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