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Start Over Author "Rampon, Claire" Journal stem cell reports
4 results on '"Rampon, Claire"'

1. Human iPSC-Derived Hippocampal Spheroids: An Innovative Tool for Stratifying Alzheimer Disease Patient-Specific Cellular Phenotypes and Developing Therapies

Author

Pomeshchik, Yuriy, primary, Klementieva, Oxana, additional, Gil, Jeovanis, additional, Martinsson, Isak, additional, Hansen, Marita Grønning, additional, de Vries, Tessa, additional, Sancho-Balsells, Anna, additional, Russ, Kaspar, additional, Savchenko, Ekaterina, additional, Collin, Anna, additional, Vaz, Ana Rita, additional, Bagnoli, Silvia, additional, Nacmias, Benedetta, additional, Rampon, Claire, additional, Sorbi, Sandro, additional, Brites, Dora, additional, Marko-Varga, György, additional, Kokaia, Zaal, additional, Rezeli, Melinda, additional, Gouras, Gunnar K., additional, and Roybon, Laurent, additional

Published
2023
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2. Human iPSC-derived hippocampal spheroids: An innovative tool for stratifying Alzheimer disease patient-specific cellular phenotypes and developing therapies

Author

Pomeshchik, Yuriy, primary, Klementieva, Oxana, additional, Gil, Jeovanis, additional, Martinsson, Isak, additional, Hansen, Marita Grønning, additional, de Vries, Tessa, additional, Sancho-Balsells, Anna, additional, Russ, Kaspar, additional, Savchenko, Ekaterina, additional, Collin, Anna, additional, Vaz, Ana Rita, additional, Bagnoli, Silvia, additional, Nacmias, Benedetta, additional, Rampon, Claire, additional, Sorbi, Sandro, additional, Brites, Dora, additional, Marko-Varga, György, additional, Kokaia, Zaal, additional, Rezeli, Melinda, additional, Gouras, Gunnar K., additional, and Roybon, Laurent, additional

Published
2021
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3. Human iPSC-Derived Hippocampal Spheroids: An Innovative Tool for Stratifying Alzheimer Disease Patient-Specific Cellular Phenotypes and Developing Therapies

Author

Pomeshchik, Yuriy, primary, Klementieva, Oxana, additional, Gil, Jeovanis, additional, Martinsson, Isak, additional, Hansen, Marita Grønning, additional, de Vries, Tessa, additional, Sancho-Balsells, Anna, additional, Russ, Kaspar, additional, Savchenko, Ekaterina, additional, Collin, Anna, additional, Vaz, Ana Rita, additional, Bagnoli, Silvia, additional, Nacmias, Benedetta, additional, Rampon, Claire, additional, Sorbi, Sandro, additional, Brites, Dora, additional, Marko-Varga, György, additional, Kokaia, Zaal, additional, Rezeli, Melinda, additional, Gouras, Gunnar K., additional, and Roybon, Laurent, additional

Published
2020
Full Text
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4. Human iPSC-Derived Hippocampal Spheroids: An Innovative Tool for Stratifying Alzheimer Disease Patient-Specific Cellular Phenotypes and Developing Therapies

Author

Yuriy Pomeshchik, Oxana Klementieva, Jeovanis Gil, Isak Martinsson, Marita Grønning Hansen, Tessa de Vries, Anna Sancho-Balsells, Kaspar Russ, Ekaterina Savchenko, Anna Collin, Ana Rita Vaz, Silvia Bagnoli, Benedetta Nacmias, Claire Rampon, Sandro Sorbi, Dora Brites, György Marko-Varga, Zaal Kokaia, Melinda Rezeli, Gunnar K. Gouras, Laurent Roybon, Lund University [Lund], Universidade de Lisboa = University of Lisbon (ULISBOA), Università degli Studi di Firenze = University of Florence (UniFI), Centre de Recherches sur la Cognition Animale - UMR5169 (CRCA), Institut des sciences du cerveau de Toulouse. (ISCT), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre de Biologie Intégrative (CBI), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre de Biologie Intégrative (CBI), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Rampon, Claire, Universidade de Lisboa (ULISBOA), Università degli Studi di Firenze = University of Florence [Firenze] (UNIFI), Centre de Recherches sur la Cognition Animale (CRCA), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut des sciences du cerveau de Toulouse. (ISCT), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse - Jean Jaurès (UT2J)-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Resource, Proteome, Transcription, Genetic, hippocampus, [SDV]Life Sciences [q-bio], Induced Pluripotent Stem Cells, NeuroD1, spheroids, viral-mediated gene therapy, Biochemistry, protein aggregation, 03 medical and health sciences, Amyloid beta-Protein Precursor, Protein Aggregates, transcriptomics, 0302 clinical medicine, proteomics, Spheroids, Cellular, Genetics, Presenilin-1, Humans, 030304 developmental biology, Neurons, 0303 health sciences, iPSC, Correction, Cell Biology, Alzheimer disease, Genetic Therapy, 3. Good health, [SDV] Life Sciences [q-bio], Phenotype, Case-Control Studies, 030217 neurology & neurosurgery, Developmental Biology
Abstract

Summary The hippocampus is important for memory formation and is severely affected in the brain with Alzheimer disease (AD). Our understanding of early pathogenic processes occurring in hippocampi in AD is limited due to tissue unavailability. Here, we report a chemical approach to rapidly generate free-floating hippocampal spheroids (HSs), from human induced pluripotent stem cells. When used to model AD, both APP and atypical PS1 variant HSs displayed increased Aβ42/Aβ40 peptide ratios and decreased synaptic protein levels, which are common features of AD. However, the two variants differed in tau hyperphosphorylation, protein aggregation, and protein network alterations. NeuroD1-mediated gene therapy in HSs-derived progenitors resulted in modulation of expression of numerous genes, including those involved in synaptic transmission. Thus, HSs can be harnessed to unravel the mechanisms underlying early pathogenic changes in the hippocampi of AD patients, and provide a robust platform for the development of therapeutic strategies targeting early stage AD., Graphical Abstract, Highlights • Rapid generation of hippocampal spheroids (HSs) from hiPSCs using defined chemical agents • hiPSC-derived HSs can be utilized as a source of hippocampal neurons • hiPSC-derived HSs can be used to model Alzheimer disease • hiPSC-derived HSs can be used to develop innovative therapeutic solutions, In this article, Roybon and colleagues developed a protocol to efficiently and rapidly generate hippocampus neurons from human induced pluripotent stem cells, which they used to model Alzheimer disease and develop a gene therapy approach to modulate the expression of genes involved in synaptic transmission.

Published
2020

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