1. Dynamic proteome profiling of human pluripotent stem cell-derived pancreatic progenitors
- Author
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Rohit N. Kulkarni, Steven P. Gygi, Andreas Alvin Purnomo Soetedjo, Adrian Kee Keong Teo, Yngvild Bjørlykke, Helge Ræder, Joanita Binte Jasmen, Larry Sai Weng Loo, Heidrun Vethe, Ivan Achel Valdez, Nicholas Jackson, Harald Barsnes, Joao A. Paulo, and Marc Vaudel
- Subjects
0301 basic medicine ,Pluripotent Stem Cells ,Proteomics ,Hippo signaling pathway ,Proteome ,Cellular differentiation ,Quantitative proteomics ,Cell Differentiation ,Cell Biology ,Computational biology ,Biology ,Cell fate determination ,Article ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Molecular Medicine ,Humans ,Stem cell ,Induced pluripotent stem cell ,Pancreas ,030217 neurology & neurosurgery ,Developmental Biology - Abstract
A comprehensive characterization of the molecular processes controlling cell fate decisions is essential to derive stable progenitors and terminally differentiated cells that are functional from human pluripotent stem cells (hPSCs). Here, we report the use of quantitative proteomics to describe early proteome adaptations during hPSC differentiation toward pancreatic progenitors. We report that the use of unbiased quantitative proteomics allows the simultaneous profiling of numerous proteins at multiple time points, and is a valuable tool to guide the discovery of signaling events and molecular signatures underlying cellular differentiation. We also monitored the activity level of pathways whose roles are pivotal in the early pancreas differentiation, including the Hippo signaling pathway. The quantitative proteomics data set provides insights into the dynamics of the global proteome during the transition of hPSCs from a pluripotent state toward pancreatic differentiation.
- Published
- 2019