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Your search keyword '"Carmela Nardelli"' showing total 2 results
Start Over Author "Carmela Nardelli" Journal stem cells and development
2 results on '"Carmela Nardelli"'

1. Altered Bioenergetic Profile in Umbilical Cord and Amniotic Mesenchymal Stem Cells from Newborns of Obese Women

Author

Carmela Nardelli, Marcella Nunziato, Lucia Sacchetti, Claudio Procaccini, Giuseppe Matarese, Lucia Mauriello, Giuseppe Maria Maruotti, Francesca D'Alessio, Pasquale Martinelli, Luigi Del Vecchio, Laura Iaffaldano, Lucio Pastore, Valeria D'Argenio, Giuseppe Labruna, Iaffaldano, Laura, Nardelli, Carmela, D'Alessio, Francesca, D'Argenio, Valeria, Nunziato, Marcella, Mauriello, Lucia, Procaccini, Claudio, Maruotti, Giuseppe Maria, Martinelli, Pasquale, Matarese, Giuseppe, Pastore, Lucio, Del Vecchio, Luigi, Labruna, Giuseppe, and Sacchetti, Lucia

Subjects
Adult, Male, 0301 basic medicine, medicine.medical_specialty, placenta, Bioenergetics, Offspring, Context (language use), Type 2 diabetes, Mitochondrion, Biology, Umbilical cord, Umbilical Cord, MSC, 03 medical and health sciences, Oxygen Consumption, Internal medicine, Placenta, Respiration, medicine, Humans, Amnion, Obesity, Infant, Newborn, amniotic stem cells, Mesenchymal Stem Cells, Cell Biology, Hematology, medicine.disease, Mitochondria, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, Female, amniotic stem cell, Energy Metabolism, Developmental Biology
Abstract

Nutritional imbalance and metabolic alterations associated with maternal obesity during pregnancy predispose offspring to obesity and/or to type 2 diabetes, but the mechanisms underlying these effects are still obscure. In this context, we evaluated whether the two main energy-producing pathways (glycolysis and mitochondrial oxidative phosphorylation) are impaired in obesity during pregnancy thus contributing to metabolic intrauterine alterations. Specifically, we studied metabolic abnormalities in the intrauterine life of newborns using stem cells isolated from amnion and umbilical cord (hA- and hUC-MSCs). We isolated, at delivery, neonatal hUC-MSCs from 13 obese (Ob) and 10 normal weight control (Co) women (prepregnancy body mass index >30 and

Published
2018
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2. High aminopeptidase N/CD13 levels characterize human amniotic mesenchymal stem cells and drive their increased adipogenic potential in obese women

Author

Maddalena Raia, Maddalena Ferrigno, Lucio Pastore, Laura Iaffaldano, Elisabetta Mariotti, Giuseppe Maria Maruotti, F. Quaglia, Pasquale Martinelli, Luigi Del Vecchio, Lucia Sacchetti, Rosa Di Noto, Giuseppe Labruna, Valentina Capobianco, Angela Capone, Carmela Nardelli, Iaffaldano, Laura, Nardelli, Carmela, Raia, M, Mariotti, E, Ferrigno, M, Quaglia, F, Labruna, G, Capobianco, Valentina, Capone, A, Maruotti, Gm, Pastore, Lucio, Di Noto, R, Martinelli, Pasquale, Sacchetti, L, and DEL VECCHIO, Luigi

Subjects
Adult, medicine.medical_specialty, Adipose tissue, Gene Expression, Biology, CD13 Antigens, Body Mass Index, Immunophenotyping, Antigen, Original Research Reports, Pregnancy, Risk Factors, Internal medicine, medicine, Humans, Amnion, Obesity, RNA, Messenger, RNA, Small Interfering, Fetus, Adipogenesis, Mesenchymal stem cell, Infant, Newborn, Mesenchymal Stem Cells, Cell Biology, Hematology, medicine.disease, PPAR gamma, Endocrinology, In utero, Case-Control Studies, Female, Body mass index, Developmental Biology
Abstract

Maternal obesity is associated to increased fetal risk of obesity and other metabolic diseases. Human amniotic mesenchymal stem cells (hA-MSCs) have not been characterized in obese women. The aim of this study was to isolate and compare hA-MSC immunophenotypes from obese (Ob-) and normal weight control (Co-) women, to identify alterations possibly predisposing the fetus to obesity. We enrolled 16 Ob- and 7 Co-women at delivery (mean/SEM prepregnancy body mass index: 40.3/1.8 and 22.4/1.0 kg/m2, respectively), and 32 not pregnant women. hA-MSCs were phenotyped by flow cytometry; several maternal and newborn clinical and biochemical parameters were also measured. The expression of membrane antigen CD13 was higher on Ob-hA-MSCs than on Co-hA-MSCs (P = 0.005). Also, serum levels of CD13 at delivery were higher in Ob- versus Co-pregnant women and correlated with CD13 antigen expression on Ob-hA-MSCs (r2 = 0.84, P < 0.0001). Adipogenesis induction experiments revealed that Ob-hA-MSCs had a higher adipogenic potential than Co-hA-MSCs as witnessed by higher peroxisome proliferator-activated receptor gamma and aP2 mRNA levels (P = 0.05 and P = 0.05, respectively), at postinduction day 14 associated with increased CD13 mRNA levels from baseline to day 4 postinduction (P < 0.05). Adipogenesis was similar in the two sets of hA-MSCs after CD13 silencing, whereas it was increased in Co-hA-MSCs after CD13 overexpression. CD13 expression was high also in Ob-h-MSCs from umbilical cords or visceral adipose tissue of not pregnant women. In conclusion, antigen CD13, by influencing the adipogenic potential of hA-MSCs, could be an in utero risk factor for obesity. Our data strengthen the hypothesis that high levels of serum and MSC CD13 are obesity markers.

Published
2013

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