1. Direct Generation of Human Cortical Organoids from Primary Cells
- Author
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Schukking, Monique, Miranda, Helen C, Trujillo, Cleber A, Negraes, Priscilla D, and Muotri, Alysson R
- Subjects
Clinical Research ,Stem Cell Research - Nonembryonic - Human ,Brain Disorders ,Stem Cell Research - Embryonic - Human ,Stem Cell Research - Induced Pluripotent Stem Cell ,Neurosciences ,Stem Cell Research - Induced Pluripotent Stem Cell - Human ,Stem Cell Research ,Underpinning research ,1.1 Normal biological development and functioning ,Neurological ,Animals ,Brain ,Cell Culture Techniques ,Cell Differentiation ,Cellular Reprogramming ,Humans ,Induced Pluripotent Stem Cells ,Organoids ,induced pluripotent stem cell ,organoids ,high-throughput ,neural development ,Biological Sciences ,Technology ,Medical and Health Sciences ,Developmental Biology ,Immunology - Abstract
The study of variations in human neurodevelopment and cognition is limited by the availability of experimental models. While animal models only partially recapitulate the human brain development, genetics, and heterogeneity, human-induced pluripotent stem cells can provide an attractive experimental alternative. However, cellular reprogramming and further differentiation techniques are costly and time-consuming and therefore, studies using this approach are often limited to a small number of samples. In this study, we describe a rapid and cost-effective method to reprogram somatic cells and the direct generation of cortical organoids in a 96-well format. Our data are a proof-of-principle that a large cohort of samples can be generated for experimental assessment of the human neural development.
- Published
- 2018