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Start Over Author "Lance, Bigelow" Journal structural dynamics
2 results on '"Lance, Bigelow"'

1. Structural dynamics of a methionine γ-lyase for calicheamicin biosynthesis: Rotation of the conserved tyrosine stacking with pyridoxal phosphate

Author

Hongnan Cao, Kemin Tan, Fengbin Wang, Lance Bigelow, Ragothaman M. Yennamalli, Robert Jedrzejczak, Gyorgy Babnigg, Craig A. Bingman, Andrzej Joachimiak, Madan K. Kharel, Shanteri Singh, Jon S. Thorson, and George N. Phillips Jr.

Subjects
Crystallography, QD901-999
Abstract

CalE6 from Micromonospora echinospora is a (pyridoxal 5′ phosphate) PLP-dependent methionine γ-lyase involved in the biosynthesis of calicheamicins. We report the crystal structure of a CalE6 2-(N-morpholino)ethanesulfonic acid complex showing ligand-induced rotation of Tyr100, which stacks with PLP, resembling the corresponding tyrosine rotation of true catalytic intermediates of CalE6 homologs. Elastic network modeling and crystallographic ensemble refinement reveal mobility of the N-terminal loop, which involves both tetrameric assembly and PLP binding. Modeling and comparative structural analysis of PLP-dependent enzymes involved in Cys/Met metabolism shine light on the functional implications of the intrinsic dynamic properties of CalE6 in catalysis and holoenzyme maturation.

Published
2016
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2. Structural dynamics of a methionine γ-lyase for calicheamicin biosynthesis: Rotation of the conserved tyrosine stacking with pyridoxal phosphate

Author

Robert Jedrzejczak, Jon S. Thorson, Fengbin Wang, Lance Bigelow, Kemin Tan, Hongnan Cao, George N. Phillips, Ragothaman M. Yennamalli, Gyorgy Babnigg, Shanteri Singh, Craig A. Bingman, Andrzej Joachimiak, and Madan K. Kharel

Subjects
0301 basic medicine, Stereochemistry, Biological Systems, ARTICLES, 03 medical and health sciences, chemistry.chemical_compound, Biosynthesis, lcsh:QD901-999, Tyrosine, Pyridoxal phosphate, Instrumentation, Pyridoxal, Spectroscopy, Radiation, Methionine, biology, Condensed Matter Physics, biology.organism_classification, Lyase, Micromonospora echinospora, 030104 developmental biology, chemistry, Biochemistry, lipids (amino acids, peptides, and proteins), Ethanesulfonic acid, lcsh:Crystallography
Abstract

CalE6 from Micromonospora echinospora is a (pyridoxal 5′ phosphate) PLP-dependent methionine γ-lyase involved in the biosynthesis of calicheamicins. We report the crystal structure of a CalE6 2-(N-morpholino)ethanesulfonic acid complex showing ligand-induced rotation of Tyr100, which stacks with PLP, resembling the corresponding tyrosine rotation of true catalytic intermediates of CalE6 homologs. Elastic network modeling and crystallographic ensemble refinement reveal mobility of the N-terminal loop, which involves both tetrameric assembly and PLP binding. Modeling and comparative structural analysis of PLP-dependent enzymes involved in Cys/Met metabolism shine light on the functional implications of the intrinsic dynamic properties of CalE6 in catalysis and holoenzyme maturation.

Published
2016

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