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Cluster memberships associated with the mediation effect are often changed due to the temporal distance between the cause-and-effect variables in longitudinal data. Nevertheless, current practices in multilevel mediation analysis mostly assume a purely hierarchical data structure. A Monte Carlo simulation study is conducted to examine the consequence of ignoring the changes in cluster memberships in multilevel mediation analysis. Results show that the proposed method, Cross-Classified Multilevel Mediation Model (CC-M3), outperforms the conventional multilevel model with substantially smaller relative biases in parameter estimates (about 50% less) and a more consistent and higher coverage rate. Findings of this simulation study inform the empirical researchers that the changes in cluster-membership needs to be appropriately taken into consideration in mediation analysis. We demonstrate the use of CC-M3 in the applied example. [ABSTRACT FROM AUTHOR]

We compared six common methods in estimating the 2-1-1 (level-2 independent, level-1 mediator, level-1 dependent) multilevel mediation model with a random slope. They were the Bayesian with informative priors, the Bayesian with non-informative priors, the Monte-Carlo, the distribution of the product, the bias-corrected, and the bias-uncorrected parametric percentile residual bootstrap. The Bayesian method with informative priors was superior in relative mean square error (RMSE), power, interval width, and interval imbalance. The prior variance and prior mean were also varied and examined. Decreasing the prior variance increased the power, reduced RMSE and interval width when the prior mean was the true value, but decreasing the prior variance reduced the power when the prior mean was set incorrectly. The influence of misspecification of prior information of the b coefficient on multilevel mediation analysis was greater than that on coefficient a. An illustrate example with the Bayesian multilevel mediation was provided. [ABSTRACT FROM AUTHOR]

Multilevel structural equation modeling (ML-SEM) for multilevel mediation is noted for its flexibility over a system of multilevel models (MLMs). Sample size requirements are an overlooked limitation of ML-SEM (100 clusters is recommended). We find that 89% of ML-SEM studies have fewer than 100 clusters and the median number is 44. Furthermore, 75% of ML-SEM studies implement 2–1–1 or 1–1–1 models, which can be equivalently fit with MLMs. MLMs theoretically have lower sample size requirements, although studies have yet to assess small sample performance for multilevel mediation. We conduct a simulation to address this pervasive problem. We find that MLMs have more desirable small sample performance and can be trustworthy with 10 clusters. Importantly, many studies lack the sample size and model complexity to necessitate ML-SEM. Although ML-SEM is undeniably more flexible and uniquely positioned for difficult problems, small samples often can be more effectively and simply addressed with MLMs. [ABSTRACT FROM AUTHOR]