1. A fluorescence turn-on detection of copper(II) based on the template-dependent click ligation of oligonucleotides.
- Author
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Wang, Fangyuan, Li, Yongxin, Li, Wenying, Chen, Jian, Zhang, Qingfeng, Anjum Shahzad, Sohail, and Yu, Cong
- Subjects
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FLUORESCENCE , *COPPER compounds , *CLICK chemistry , *OLIGONUCLEOTIDES , *VITAMIN C , *RING formation (Chemistry) - Abstract
In this work, a fluorescence turn-on method for copper(II) detection is reported. A molecular beacon (MB) was designed as a template. Cu 2+ was reduced to Cu + in the presence of a reductant (ascorbic acid). Two short single-stranded oligonucleotides one was labeled with a 5′-alkyne and the other with 3′-azide group, proceeded a template-dependent chemical ligation through the Cu(I)-catalyzed azide-alkyne cycloaddition. The newly generated click-ligated long chain oligonucleotide, which was complementary to the MB, opened the MB hairpin structure and resulted in a turn on fluorescence. The increase in fluorescence intensity is directly proportional to the amount of Cu 2+ added to the assay solution. The present assay is quite sensitive and allows the detection of 2 nM Cu 2+ . The described assay also exhibits high selectivity over other metal ions. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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