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In this work, molecularly imprinted electrospun chromogenic membrane(MIP-ECM) was synthesized using P -Hydroxybenzene propanoic acid as imitate template molecule and Poly (vinyl alcohol)(PVA) as substrate. Ninhydrin is used as chromogenic agent for visualizing detection of target molecule l -tyrosine(Tyr). The results showed that molecularly imprinted membrane exhibited selectivity and specific adsorption capacity for l -tyrosine. The influences of environmental factors, such as reaction temperature, the amount of ninhydrin and elution time, on chromogenic properties were examined by SEM and optical microscope. Gray value analysis is proceeded by software Image J. Due to the facilitate preparation and intuitive visualization, the proposed method was a promising candidate for selective recognition of bio-generated acid and amino molecules. Image 1 • The imprinting and coloring structure is constructed in nanofiber membrane with large surface area. • The adsorption is visualized by chromogenic reaction to form a 3D interactive surface proceeded by ImageJ. • The test process is fast and not it is not necessary to sample the subject for UV spectrum absorption. [ABSTRACT FROM AUTHOR]