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In this work, molecularly imprinted electrospun chromogenic membrane(MIP-ECM) was synthesized using P-Hydroxybenzene propanoic acid as imitate template molecule and Poly (vinyl alcohol)(PVA) as substrate. Ninhydrin is used as chromogenic agent for visualizing detection of target molecule l-tyrosine(Tyr). The results showed that molecularly imprinted membrane exhibited selectivity and specific adsorption capacity for l-tyrosine. The influences of environmental factors, such as reaction temperature, the amount of ninhydrin and elution time, on chromogenic properties were examined by SEM and optical microscope. Gray value analysis is proceeded by software Image J. Due to the facilitate preparation and intuitive visualization, the proposed method was a promising candidate for selective recognition of bio-generated acid and amino molecules., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Bovine serum albumin (BSA) imprinted polyampholyte hydrogels (PAHs) were prepared by free radical polymerization using acrylamide (Am) as structural monomer, N-isopropylacrylamide (NIPAm), [2-(methacryloyloxy)ethyl]trimethylammonium chloride (DMC) and 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) as functional monomers and N,N'-methylenebisacrylamide (MBA) as crosslinker in aqueous solution. The morphology of imprinted hydrogels and non-imprinted hydrogels were characterized by scanning electron microscope (SEM). The adsorption and recognition properties were evaluated as functions of Am monomer concentration, NIPAm/Am molar ratio, crosslinking structure and charge density ratio etc. The adsorption capacity and association constant of specific interaction between hydrogel and template protein were analyzed by Langmuir isotherm model and Freundlich model. The fitting experimental data suggested that this adsorption was better described as a monolayer adsorption. The specific adsorption on hydrogel with different crosslinking structure was investigated by selective binding BSA from single solution and binary mixture solution. The charge density ratio in molecular imprinting hydrogel had obvious influence for protein adsorption and recognition. The resultant of regeneration tests showed that elution had large impact on deterioration of the imprinting structure., (Copyright © 2018 Elsevier B.V. All rights reserved.)