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In this work, a dicyanoisophorone-based turn-on fluorescent probe, DCIP , for highly selective and sensitive detection of cysteine was designed based on nucleophilic substitution mechanism. Moreover, compared with typical cysteine probes, DCIP showed great selectivity and sensitivity for cysteine over other amino acids including the similar structured homocysteine (Hcy) and glutathione (GSH). Further, the detection limit toward cysteine was calculated to be as low as 0.70 μM. In addition, the utility of DCIP as a bioanalytical molecular tool was demonstrated by fluorescence imaging of biothiols in living cells. [ABSTRACT FROM AUTHOR]

Development of an excited-state intramolecular proton transfer (ESIPT)-based fluorescent probe ( BIHM ) for selective recognition to homocysteine (Hcy) over glutathione (GSH), cysteine (Cys) and other amino acids is described. The distinctive fluorescence response of BIHM to Hcy over Cys is interpreted by density functional theory calculations. Bioimaging experiments of intracellular Hcy detection manifest the great potential utility of the probe. [ABSTRACT FROM AUTHOR]