1. Effect of Sutellarin on Neurogenesis in Neonatal Hypoxia–Ischemia Rat Model: Potential Mechanisms of Action
- Author
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Jia Liu, Yuan Peng, Donghui Liu, Li Chen, Ya-Xin Tan, Larisa Bobrovskaya, Liu-Lin Xiong, Ruo-Lan Du, Xin-Fu Zhou, Lu-Lu Xue, Ting-Hua Wang, Mohammed Al-Hawwas, Xiong, Liu Lin, Tan, Ya Xin, Du, Ruo Lan, Peng, Yuan, Xue, Lu Lu, Liu, Jia, Al-Hawwas, Mohammed, Bobrovskaya, Larisa, Liu, Dong Hui, Chen, Li, Wang, Ting Hua, and Zhou, Xin Fu
- Subjects
0301 basic medicine ,Neurite ,Neurogenesis ,Neuronal Outgrowth ,Hippocampus ,Apoptosis ,Glucuronates ,Nerve Tissue Proteins ,therapeutic efficacy ,Andrology ,03 medical and health sciences ,symbols.namesake ,0302 clinical medicine ,neonatal hypoxic-ischemic ,Autophagy ,medicine ,Animals ,Receptors, Growth Factor ,Viability assay ,Apigenin ,Cells, Cultured ,Neurons ,biology ,Microglia ,Chemistry ,Brain ,General Medicine ,scutellarin ,Rats ,Disease Models, Animal ,030104 developmental biology ,medicine.anatomical_structure ,Animals, Newborn ,nervous system ,Complementary and alternative medicine ,Hypoxia-Ischemia, Brain ,biology.protein ,Nissl body ,symbols ,NeuN ,030217 neurology & neurosurgery ,Immunostaining - Abstract
To investigate the therapeutic efficacy of Scutellarin (SCU) on neurite growth and neurological functional recovery in neonatal hypoxic-ischemic (HI) rats. Primary cortical neurons were cultured to detect the effect of SCU on cell viability of neurons under oxygen-glucose deprivation (OGD). Double immunofluorescence staining of Tuj1 and TUNEL then observed the neurite growth and cell apoptosis in vitro,and double immunofluorescence staining of NEUN and TUNEL was performed to examine the neuronal apoptosis and cell apoptosis in brain tissues after HI in vivo. Pharmacological efficacy of SCU was also evaluated in HI rats by neurobehavioral tests, triphenyl tetrazolium chloride staining, Hematoxylin and eosin staining and Nissl staining. Astrocytes and microglia expression in damaged brain tissues were detected by immunostaining of GFAP and Iba1. A quantitative real-time polymerase chain reaction and western blot were applied to investigate the genetic expression changes and the protein levels of autophagy-related proteins in the injured cortex and hippocampus after HI. We found that SCU administration preserved cell viability, promoted neurite outgrowth and suppressed apoptosis of neurons subjected to OGD both in vitroand in vivo. Meanwhile, 20 mg/kg SCU treatment improved neurological functions and decreased the expression of astrocytes and microglia in the cortex and hippocampus of HI rats. Additionally, SCU treatment depressed the elevated levels of autophagy-related proteins and the p75 neurotrophin receptor (p75NTR) in both cortex and hippocampus. This study demonstrated the potential therapeutic efficacy of SCU by enhancing neurogenesis and restoring long-term neurological dysfunctions, which might be associated with p75NTR depletion in HI rats. Refereed/Peer-reviewed
- Published
- 2021