1. Choroid plexus‐derived miR‐204 regulates the number of quiescent neural stem cells in the adult brain
- Author
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Tamara Müller, Dorothea Schulte, Jovica Ninkovic, Melanie Pusch, Tjasa Lepko, Enric Llorens-Bobadilla, Janina Ehses, Andre Fischer, Ana Martin-Villalba, Roosmarijn E. Vandenbroucke, Miha Modic, Magdalena Götz, Charysse Vandendriessche, Julia Hasler, Sheng Zhao, Stefan H. Stricker, Anja Schneider, Christopher T. Breunig, Hagen B. Huttner, and Michael A. Kiebler
- Subjects
Adult Neurogenesis ,Mir-204 ,Neural Stem Cells ,Neurogenesis ,Neurogenic Priming ,Adult ,Male ,chemistry [Choroid Plexus] ,MIRN204 microRNA, mouse ,Subventricular zone ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Cerebrospinal fluid ,Cell Movement ,ddc:570 ,medicine ,Subependymal zone ,Animals ,Humans ,News & Views ,genetics [MicroRNAs] ,cytology [Neural Stem Cells] ,Stem Cell Niche ,Progenitor cell ,Molecular Biology ,030304 developmental biology ,0303 health sciences ,General Immunology and Microbiology ,General Neuroscience ,Cell Cycle ,Cell Differentiation ,Middle Aged ,Neural stem cell ,Cell biology ,MIRN204 microRNA, human ,MicroRNAs ,medicine.anatomical_structure ,Gene Expression Regulation ,Choroid Plexus ,chemistry [Neural Stem Cells] ,cerebrospinal fluid [MicroRNAs] ,Female ,Choroid plexus ,Stem cell ,030217 neurology & neurosurgery - Abstract
Regulation of adult neural stem cell (NSC) number is critical for lifelong neurogenesis. Here, we identified a post-transcriptional control mechanism, centered around the microRNA 204 (miR-204), to control the maintenance of quiescent (q)NSCs. miR-204 regulates a spectrum of transcripts involved in cell cycle regulation, neuronal migration, and differentiation in qNSCs. Importantly, inhibition of miR-204 function reduced the number of qNSCs in the subependymal zone (SEZ) by inducing pre-mature activation and differentiation of NSCs without changing their neurogenic potential. Strikingly, we identified the choroid plexus of the mouse lateral ventricle as the major source of miR-204 that is released into the cerebrospinal fluid to control number of NSCs within the SEZ. Taken together, our results describe a novel mechanism to maintain adult somatic stem cells by a niche-specific miRNA repressing activation and differentiation of stem cells.
- Published
- 2019
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