1. Independent regulation of HNF-1 alpha and HNF-1 beta by retinoic acid in F9 teratocarcinoma cells
- Author
-
Dirk B. Mendel, Gerald R. Crabtree, Linda P. Hansen, and Calvin J. Kuo
- Subjects
Embryonal Carcinoma Stem Cells ,Transcription, Genetic ,Molecular Sequence Data ,Retinoic acid ,Biology ,DNA-binding protein ,digestive system ,General Biochemistry, Genetics and Molecular Biology ,Embryonal carcinoma ,chemistry.chemical_compound ,medicine ,Tumor Cells, Cultured ,Animals ,RNA, Messenger ,Promoter Regions, Genetic ,Molecular Biology ,Transcription factor ,Hepatocyte Nuclear Factor 1-beta ,Regulation of gene expression ,General Immunology and Microbiology ,Base Sequence ,General Neuroscience ,Nuclear Proteins ,Promoter ,DNA ,medicine.disease ,Molecular biology ,DNA Fingerprinting ,DNA-Binding Proteins ,chemistry ,Gene Expression Regulation ,Retinoic acid receptor alpha ,embryonic structures ,Hepatocyte Nuclear Factor 1 ,Hepatocyte Nuclear Factor 1-Beta ,Neoplastic Stem Cells ,Transcription Factors ,Research Article - Abstract
Hepatocyte Nuclear Factor-1 alpha (HNF-1 alpha) and HNF-1 beta are homeodomain-containing transcription factors which interact with the GTTAATNATTAAC motif essential to the function of more than 15 promoters selectively expressed in the liver. These homeoproteins can form homo- and heterodimers in solution and share identical DNA-binding domains but have different transcriptional activation properties. During retinoic acid (RA) induced differentiation of F9 embryonal carcinoma (EC) cells, which stimulates aspects of pre-implantation embryogenesis, both HNF-1 beta mRNA and immunoreactive DNA-binding activity are strongly induced approximately 24 h post RA-treatment. In contrast, HNF-1 alpha mRNA increases approximately 4-fold after 5 days, concomitant with elevation of HNF-1 alpha DNA-binding activity and expression of the HNF-1 target gene alpha-fetoprotein. These results indicate that HNF-1 alpha and -1 beta expression can be controlled by regulatory hierarchies downstream of primary RA-response genes, and suggest that independent regulatory mechanisms for these factors can confer distinct and interactive developmental functions.
- Published
- 1991