1. An altered response in macrophage phenotype following damage in aged human skeletal muscle: implications for skeletal muscle repair
- Author
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Paul S. Hafen, Michael R. Deyhle, Robert D. Hyldahl, Jacob R. Sorensen, Jamie P. Kaluhiokalani, and Allen C. Parcell
- Subjects
Adult ,Male ,0301 basic medicine ,Aging ,Macrophage polarization ,Inflammation ,Biology ,Biochemistry ,Myoblasts ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Genetics ,medicine ,Humans ,Macrophage ,Muscle, Skeletal ,Exercise ,Molecular Biology ,Cells, Cultured ,Aged ,Macrophages ,Skeletal muscle ,Macrophage Activation ,biology.organism_classification ,Phenotype ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,Female ,Satellite (biology) ,Cell response ,medicine.symptom ,030217 neurology & neurosurgery ,Biotechnology - Abstract
The purpose of this study was to test the hypothesis that macrophage polarization is altered in old compared to young skeletal muscle, possibly contributing to the poor satellite cell response observed in older muscle tissue. Muscle biopsies were collected prior to and at 3, 24, and 72 h following a muscle-damaging exercise in young and old individuals. Immunohistochemistry was used to measure i.m. macrophage content and phenotype, and cell culture experiments tested macrophage behavior and influence on primary myoblasts from older individuals. We found that macrophage infiltration was similar between groups at 24 (young: 3712 ± 2407
- Published
- 2019