1. Whole cellculture filtrate proteins from prevalent genotypes of Mycobacterium tuberculosis provoke better antibodyT cell response than laboratory strain H 37 Rv
- Author
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Gavish, Kumar, Hari, Shankar, Mamta, Chahar, Pragya, Sharma, Virendra Singh, Yadav, Devendra Singh, Chauhan, Vishwa Mohan, Katoch, and Beenu, Joshi
- Subjects
Adult ,Cell Extracts ,Male ,Immunity, Cellular ,Adolescent ,Genotype ,T-Lymphocytes ,Mycobacterium tuberculosis ,whole cell extract ,Middle Aged ,Antibodies ,Bacterial Proteins ,clinical Mycobacterium tuberculosis isolates ,Humans ,Tuberculosis ,Female ,Original Article ,culture filtrate protein ,Cell Proliferation ,Antibody and cell mediated response - Abstract
Background & objectives: The immune responses to different antigens of Mycobacterium tuberculosis H37Rv vary from patient to patient with tuberculosis (TB). Therefore, significant difference might be documented between the H37Rv with long histories of passages and recent clinical isolates of M. tuberculosis. In the present study, immune response of TB patients and healthy controls against 39 clinical M. tuberculosis isolates was correlated with laboratory strain H37Rv. Methods: The antibody response was studied coating whole cell extracts and culture filtrate proteins of M. tuberculosis isolates and laboratory strain H37Rv by enzyme linked immunosorbent assay (ELISA). Lymphoproliferation was studied by incorporation of tritiated thymidine and cytokines (IFN-γ and IL-4) by using commercially available kits. Results: Sero-reactivity to whole cell extract (WCE) of 11 clinical isolates was higher with pooled serum and individual's serum from tuberculosis patients showed significant reactivity (P
- Published
- 2012