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Your search keyword '"Georas, Steve N."' showing total 15 results
Start Over Author "Georas, Steve N." Journal the journal of allergy and clinical immunology
15 results on '"Georas, Steve N."'

3. Tight junction defects in patients with atopic dermatitis

Author

De Benedetto, Anna, Rafaels, Nicholas M., McGirt, Laura Y., Ivanov, Andrei I., Georas, Steve N., Cheadle, Chris, Berger, Alan E., Zhang, Kunzhong, Vidyasagar, Sadasivan, Yoshida, Takeshi, Boguniewicz, Mark, Hata, Tissa, Schneider, Lynda C., Hanifin, Jon M., Gallo, Richard L., Novak, Natalija, Weidinger, Stephan, Beaty, Terri H., Leung, Donald Y.M., Barnes, Kathleen C., and Beck, Lisa A.

Published
2011
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4. The role of ST2 and ST2 genetic variants in schistosomiasis

Author

Long, Xin, Daya, Michelle, Zhao, Jianping, Rafaels, Nicholas, Liang, Huifang, Potee, Joseph, Campbell, Monica, Zhang, Bixiang, Araujo, Maria Ilma, Oliveira, Ricardo R., Mathias, Rasika A., Gao, Li, Ruczinski, Ingo, Georas, Steve N., Vercelli, Donata, Beaty, Terri H., Barnes, Kathleen C., Chen, Xiaoping, and Chen, Qian

Published
2017
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15. Histone deacetylation inhibits IL4gene expression in T cells

Author

Valapour, Maryam, Guo, Jia, Schroeder, John T., Keen, Judith, Cianferoni, Antonella, Casolaro, Vincenzo, and Georas, Steve N.

Abstract

Background:Dysregulated expression of IL-4 has been linked with allergic diseases. IL-4 expression is controlled at the level of gene transcription by the coordinated action of multiple factors that bind regulatory promoter elements. In addition, alterations in chromatin structure are thought to play a role in regulating the expression of cytokines in the TH2 gene cluster, although the biochemical basis for these alterations in human T cells is not well understood. Objective:We sought to define the role of histone acetylation in the regulation of IL4gene expression in human T cells. Methods:IL-4 protein production was measured by means of ELISA. IL-4 promoter activity was measured with luciferase-based reporter constructs transiently transfected into Jurkat T cells. The acetylation status of histones associated with the IL4gene was analyzed with chromatin immunoprecipitation assays. Results:IL-4 production from activated peripheral blood T cells was enhanced by the histone deacetylase inhibitor trichostatin A. Overexpression of the type 1 histone deacetylases 1, 2, and 3 inhibited transcription driven by the IL-4 promoter in Jurkat T cells, whereas cotransfection of the histone acetyltransferase CREB-binding protein potentiated IL-4 promoter activity. Using chromatin immunoprecipitation assays, we show that nucleosomes in the proximal IL-4 promoter are acetylated on T-cell activation. Conclusion:Our results demonstrate that the acetylation state of histones associated with the IL-4 promoter is a key regulator of IL4gene expression. (J Allergy Clin Immunol 2002;109:238-45.)

Published
2002
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