1. The Polycomb Repressive Complex 1 Protein BMI1 Is Required for Constitutive Heterochromatin Formation and Silencing in Mammalian Somatic Cells
- Author
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Jida El Hajjar, Gilbert Bernier, Roy Hanna, Mohamed Abdouh, and Anthony Flamier
- Subjects
0301 basic medicine ,Euchromatin ,Heterochromatin ,Nuclear Envelope ,macromolecular substances ,Biochemistry ,Histones ,03 medical and health sciences ,Mice ,Non-histone protein ,Neural Stem Cells ,Proto-Oncogene Proteins ,Constitutive heterochromatin ,Animals ,Humans ,Gene Silencing ,Molecular Biology ,Pericentric heterochromatin ,Repetitive Sequences, Nucleic Acid ,Genetics ,Cerebral Cortex ,Mammals ,Neurons ,Polycomb Repressive Complex 1 ,biology ,BRCA1 Protein ,Ubiquitin ,EZH2 ,fungi ,Cell Biology ,030104 developmental biology ,Histone ,Gene Knockdown Techniques ,biology.protein ,Heterochromatin protein 1 - Abstract
The polycomb repressive complex 1 (PRC1), containing the core BMI1 and RING1A/B proteins, mono-ubiquitinylates histone H2A (H2A(ub)) and is associated with silenced developmental genes at facultative heterochromatin. It is, however, assumed that the PRC1 is excluded from constitutive heterochromatin in somatic cells based on work performed on mouse embryonic stem cells and oocytes. We show here that BMI1 is required for constitutive heterochromatin formation and silencing in human and mouse somatic cells. BMI1 was highly enriched at intergenic and pericentric heterochromatin, co-immunoprecipitated with the architectural heterochromatin proteins HP1, DEK1, and ATRx, and was required for their localization. In contrast, BRCA1 localization was BMI1-independent and partially redundant with that of BMI1 for H2A(ub) deposition, constitutive heterochromatin formation, and silencing. These observations suggest a dynamic and developmentally regulated model of PRC1 occupancy at constitutive heterochromatin, and where BMI1 function in somatic cells is to stabilize the repetitive genome.
- Published
- 2015