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Start Over Author "Ayana, T" Journal the journal of cell biology
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3. MCAK associates with the tips of polymerizing microtubules

Author

Michael Wagenbach, Annie Andrieux, Kathleen E. Rankin, George von Dassow, Didier Job, Linda Wordeman, Yulia Ovechkina, Ayana T. Moore, Leticia Peris, Department of Physiology and Biophysics, University of Washington [Seattle], Center for Cell Dynamics, Organisation Fonctionnelle du Cytosquelette, Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR27, Collaboration, and Andrieux, Annie

Subjects
MESH: Protein Transport, Polymers, Recombinant Fusion Proteins, Green Fluorescent Proteins, MESH: Cricetinae, Kinesins, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, macromolecular substances, CHO Cells, Biology, Microtubules, 03 medical and health sciences, MESH: Protein Structure, Tertiary, MESH: Cell Compartmentation, 0302 clinical medicine, MESH: Green Fluorescent Proteins, Microtubule, MESH: CHO Cells, Report, Cricetinae, Cell polarity, MESH: Recombinant Fusion Proteins, Animals, Humans, MESH: Animals, Phosphorylation, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, Research Articles, 030304 developmental biology, 0303 health sciences, MESH: Humans, Microscopy, Video, MESH: Phosphorylation, Extramural, MESH: Microtubules, Cell Polarity, Cell Biology, Cell biology, MESH: Polymers, Cell Compartmentation, Protein Structure, Tertiary, MESH: Hela Cells, MESH: Microscopy, Video, Protein Transport, Kinesin, MESH: Cell Polarity, MESH: Kinesin, 030217 neurology & neurosurgery, HeLa Cells
Abstract

International audience; MCAK is a member of the kinesin-13 family of microtubule (MT)-depolymerizing kinesins. We show that the potent MT depolymerizer MCAK tracks (treadmills) with the tips of polymerizing MTs in living cells. Tip tracking of MCAK is inhibited by phosphorylation and is dependent on the extreme COOH-terminal tail of MCAK. Tip tracking is not essential for MCAK's MT-depolymerizing activity. We propose that tip tracking is a mechanism by which MCAK is preferentially localized to regions of the cell that modulate the plus ends of MTs.

Published
2005

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