1. Selection for reproduction under short photoperiods changes diapause-associated traits and induces widespread genomic divergence
- Author
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Johanna Kinnunen, Anna-Lotta Hiillos, Hannele Kauranen, Anneli Hoikkala, R. Axel W. Wiberg, Pekka Lankinen, Michael G. Ritchie, David Hopkins, NERC, University of St Andrews. School of Biology, University of St Andrews. Centre for Biological Diversity, and University of St Andrews. Institute of Behavioural and Neural Sciences
- Subjects
0106 biological sciences ,Physiology ,030310 physiology ,QH301 Biology ,Circadian clock ,Genome, Insect ,01 natural sciences ,talvehtiminen ,kylmänkestävyys ,circadian clock ,media_common ,vuorokausirytmi ,0303 health sciences ,luonnonvalinta ,genome analyses ,Reproduction ,Phenotype ,Adaptation, Physiological ,Circadian Rhythm ,Cold Temperature ,Drosophila melanogaster ,Female ,Locomotion ,endocrine system ,mahlakärpäset ,Period (gene) ,media_common.quotation_subject ,Photoperiod ,Zoology ,reproductive diapause ,fotobiologia ,Aquatic Science ,Diapause ,Biology ,010603 evolutionary biology ,03 medical and health sciences ,QH301 ,Quantitative Trait, Heritable ,photoperiodic timer ,Animals ,Circadian rhythm ,Molecular Biology ,Gene ,Ecology, Evolution, Behavior and Systematics ,Selection (genetic algorithm) ,Critical day lenght ,Genetic Variation ,DAS ,cold tolerance ,lisääntyminen ,Chromosomes, Insect ,critical day length ,Insect Science ,perimä ,hyönteiset ,Linear Models ,Animal Science and Zoology - Abstract
The work has been supported by the Academyof Finland to A.H. (project 267244) and Natural Environment Research Council (NERC) funding (NE/J020818/1 to M.G.R.; NE/L501852/1 to R.A.W.W.). The incidence of reproductive diapause is a critical aspect of life history in overwintering insects from temperate regions. Much has been learned about the timing, physiology and genetics of diapause in a range of insects, but how the multiple changes involved in this and other photoperiodically regulated traits are inter-related is not well understood. We performed quasinatural selection on reproduction under short photoperiods in a northern fly species, Drosophila montana, to trace the effects of photoperiodic selection on traits regulated by the photoperiodic timer and/or by a circadian clock system. Selection changed several traits associated with reproductive diapause, including the critical day length for diapause (CDL), the frequency of diapausing females under photoperiods that deviate from daily 24 h cycles and cold tolerance, towards the phenotypes typical of lower latitudes. However, selection had no effect on the period of free-running locomotor activity rhythm regulated by the circadian clock in fly brain. At a genomic level, selection induced extensive divergence from the control line in 16 gene clusters involved in signal transduction, membrane properties, immunologlobulins and development. These changes resembled those detected between latitudinally divergent D. montana populations in the wild and involved SNP divergence associated with several genes linked with diapause induction. Overall, our study shows that photoperiodic selection for reproduction under short photoperiods affects diapause-associated traits without disrupting the central clock network generating circadian rhythms in fly locomotor activity. Publisher PDF
- Published
- 2019