1. Pulmonary Th17 Antifungal Immunity Is Regulated by the Gut Microbiome
- Author
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McAleer, Jeremy P, Nguyen, Nikki LH, Chen, Kong, Kumar, Pawan, Ricks, David M, Binnie, Matthew, Armentrout, Rachel A, Pociask, Derek A, Hein, Aaron, Yu, Amy, Vikram, Amit, Bibby, Kyle, Umesaki, Yoshinori, Rivera, Amariliz, Sheppard, Dean, Ouyang, Wenjun, Hooper, Lora V, and Kolls, Jay K
- Subjects
Biological Sciences ,Biomedical and Clinical Sciences ,Microbiology ,Immunology ,Medical Microbiology ,Infectious Diseases ,Emerging Infectious Diseases ,Lung ,Microbiome ,2.2 Factors relating to the physical environment ,1.1 Normal biological development and functioning ,Oral and gastrointestinal ,Inflammatory and immune system ,Infection ,Animals ,Anti-Bacterial Agents ,Aspergillosis ,Aspergillus fumigatus ,Cells ,Cultured ,Gastrointestinal Microbiome ,Gram-Positive Bacteria ,Immunity ,Interleukins ,Mice ,Mice ,Inbred C57BL ,Mice ,Knockout ,Pancreatitis-Associated Proteins ,Proteins ,Th17 Cells ,Vancomycin ,Interleukin-22 ,Biochemistry and cell biology - Abstract
Commensal microbiota are critical for the development of local immune responses. In this article, we show that gut microbiota can regulate CD4 T cell polarization during pulmonary fungal infections. Vancomycin drinking water significantly decreased lung Th17 cell numbers during acute infection, demonstrating that Gram-positive commensals contribute to systemic inflammation. We next tested a role for RegIIIγ, an IL-22-inducible antimicrobial protein with specificity for Gram-positive bacteria. Following infection, increased accumulation of Th17 cells in the lungs of RegIIIγ(-/-) and Il22(-/-) mice was associated with intestinal segmented filamentous bacteria (SFB) colonization. Although gastrointestinal delivery of rRegIIIγ decreased lung inflammatory gene expression and protected Il22(-/-) mice from weight loss during infection, it had no direct effect on SFB colonization, fungal clearance, or lung Th17 immunity. We further show that vancomycin only decreased lung IL-17 production in mice colonized with SFB. To determine the link between gut microbiota and lung immunity, serum-transfer experiments revealed that IL-1R ligands increase the accumulation of lung Th17 cells. These data suggest that intestinal microbiota, including SFB, can regulate pulmonary adaptive immune responses.
- Published
- 2016