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Perturbations in B cells occur in both, chronological aging and in HIV infection. We investigated B cells in young (Y

HIV infected persons often exhibit poor responses to influenza vaccine despite effective HAART-mediated viral suppression. The goal of the present study was to identify gene expression profiles that characterize vaccine driven antibody responses in aviremic pediatric and adolescent HIV infected patients (4-25 yrs) given two doses of Novartis/H1N1 09 vaccine 21 days apart during the pandemic H1N1 influenza outbreak in 2009. Blood samples were collected in Paxgene tubes on days 0, 21, and 31 post-vaccination for microarray analysis. Supervised clustering analysis for contrasts between vaccine responders (R) and vaccine non-responders (NR) was performed and top pathways were ranked by Ingenuity Pathway analysis. Cutoffs for fold change (1.3) and p value (0.05) were applied to assess the top upregulated and downregulated genes in pathways. Patients were segregated by age into 2 groups for analysis: 1)